Ochratoxin a determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography
Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficien...
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| Veröffentlicht in: | Ciência e tecnologia de alimentos 2003-12, Vol.23 (suppl), p.58-61 |
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| container_title | Ciência e tecnologia de alimentos |
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| creator | Prado, G Oliveira, M.S Carvalho, E.P Lima, L.C.O Veloso, T Souza, L.A.F Cardoso, A.C.F |
| description | Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficient of variation between 0% e 11.0%. Detection limit and quantification limit were 2.0pg/mL and 8.0pg/mL, respectively. Of the total of 26 samples produced in Brazil only 6 (23%), contained trace amounts of OTA. Of the 4 imported beers, in 2, Ireland and Germany, were detected OTA at levels of 25pg/mL and 82pg/mL, respectively.
Análises de ocratoxina A em cervejas nacionais e importadas foram executadas por coluna de imunoafinidade e cromatografia líquida de alta eficiência (CLAE) com detetor de fluorescência. Recuperações de ocratoxina A em amostras de cervejas contaminadas em níveis de 8.0 a 800pg/mL, foram de 81,2% a 95%, com coeficiente de variação entre 0% e 11%. O limite de detecção e o de quantificação foram 2.0pg/mL e 8.0pg/mL, respectivamente. De um total de 26 amostras fabricadas no Brasil, somente 6 (23%) apresentavam traços (pg/mL) de ocratoxina A. Das 4 amostras de cerveja importadas analisadas, em duas, Irlanda e Alemanha, foram detectadas ocratoxina A em níveis de 25pg/mL e 82pg/mL, respectivamente. |
| doi_str_mv | 10.1590/S0101-20612003000400011 |
| format | Article |
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Análises de ocratoxina A em cervejas nacionais e importadas foram executadas por coluna de imunoafinidade e cromatografia líquida de alta eficiência (CLAE) com detetor de fluorescência. Recuperações de ocratoxina A em amostras de cervejas contaminadas em níveis de 8.0 a 800pg/mL, foram de 81,2% a 95%, com coeficiente de variação entre 0% e 11%. O limite de detecção e o de quantificação foram 2.0pg/mL e 8.0pg/mL, respectivamente. De um total de 26 amostras fabricadas no Brasil, somente 6 (23%) apresentavam traços (pg/mL) de ocratoxina A. Das 4 amostras de cerveja importadas analisadas, em duas, Irlanda e Alemanha, foram detectadas ocratoxina A em níveis de 25pg/mL e 82pg/mL, respectivamente.</description><identifier>ISSN: 0101-2061</identifier><identifier>ISSN: 1678-457X</identifier><identifier>EISSN: 1678-457X</identifier><identifier>DOI: 10.1590/S0101-20612003000400011</identifier><language>eng</language><publisher>Sociedade Brasileira de Ciência e Tecnologia de Alimentos</publisher><subject>ANALISE QUIMICA ; beer ; BEERS ; BIÈRE ; CERVEJA ; CERVEZAS ; CHEMICAL COMPOSITION ; CHROMATOGRAPHIE ; CHROMATOGRAPHY ; CLAE ; COMPOSICIÓN QUIMICA ; COMPOSITION CHIMIQUE ; CROMATOGRAFIA ; CROMATOGRAFÍA ; FOOD SCIENCE & TECHNOLOGY ; HPLC ; immunoaffinity ; imunoafinidade ; MICOTOXINA ; MICOTOXINAS ; MYCOTOXINE ; MYCOTOXINS ; ochratoxin A ; OCHRATOXINE ; OCHRATOXINS ; OCRATOXINA ; ocratoxina A ; OCRATOXINAS</subject><ispartof>Ciência e tecnologia de alimentos, 2003-12, Vol.23 (suppl), p.58-61</ispartof><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2341-fe060194635701020462f8feae9fe18e6fb1d468b0440f1d05510387f79401573</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids></links><search><creatorcontrib>Prado, G</creatorcontrib><creatorcontrib>Oliveira, M.S</creatorcontrib><creatorcontrib>Carvalho, E.P</creatorcontrib><creatorcontrib>Lima, L.C.O</creatorcontrib><creatorcontrib>Veloso, T</creatorcontrib><creatorcontrib>Souza, L.A.F</creatorcontrib><creatorcontrib>Cardoso, A.C.F</creatorcontrib><title>Ochratoxin a determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography</title><title>Ciência e tecnologia de alimentos</title><addtitle>Food Sci. Technol</addtitle><description>Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficient of variation between 0% e 11.0%. Detection limit and quantification limit were 2.0pg/mL and 8.0pg/mL, respectively. Of the total of 26 samples produced in Brazil only 6 (23%), contained trace amounts of OTA. Of the 4 imported beers, in 2, Ireland and Germany, were detected OTA at levels of 25pg/mL and 82pg/mL, respectively.
Análises de ocratoxina A em cervejas nacionais e importadas foram executadas por coluna de imunoafinidade e cromatografia líquida de alta eficiência (CLAE) com detetor de fluorescência. Recuperações de ocratoxina A em amostras de cervejas contaminadas em níveis de 8.0 a 800pg/mL, foram de 81,2% a 95%, com coeficiente de variação entre 0% e 11%. O limite de detecção e o de quantificação foram 2.0pg/mL e 8.0pg/mL, respectivamente. De um total de 26 amostras fabricadas no Brasil, somente 6 (23%) apresentavam traços (pg/mL) de ocratoxina A. Das 4 amostras de cerveja importadas analisadas, em duas, Irlanda e Alemanha, foram detectadas ocratoxina A em níveis de 25pg/mL e 82pg/mL, respectivamente.</description><subject>ANALISE QUIMICA</subject><subject>beer</subject><subject>BEERS</subject><subject>BIÈRE</subject><subject>CERVEJA</subject><subject>CERVEZAS</subject><subject>CHEMICAL COMPOSITION</subject><subject>CHROMATOGRAPHIE</subject><subject>CHROMATOGRAPHY</subject><subject>CLAE</subject><subject>COMPOSICIÓN QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>CROMATOGRAFIA</subject><subject>CROMATOGRAFÍA</subject><subject>FOOD SCIENCE & TECHNOLOGY</subject><subject>HPLC</subject><subject>immunoaffinity</subject><subject>imunoafinidade</subject><subject>MICOTOXINA</subject><subject>MICOTOXINAS</subject><subject>MYCOTOXINE</subject><subject>MYCOTOXINS</subject><subject>ochratoxin A</subject><subject>OCHRATOXINE</subject><subject>OCHRATOXINS</subject><subject>OCRATOXINA</subject><subject>ocratoxina A</subject><subject>OCRATOXINAS</subject><issn>0101-2061</issn><issn>1678-457X</issn><issn>1678-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNp9kN1Kw0AQhRdRsFYfQdwXSJ3JbrLJpRb_oFCwCr0Lm2S32ZLsxk0D5u1NW_FG8WIYmDnfnOEQcoMwwyiF2xUgYBBCjCEAAwA-FuIJmWAskoBHYn1KJj-ic3LRdVuAUDDOJsQti8rLnfs0lkpaqp3yjbFyZ5yl4yhXytN8oKZpeuuk1saa3UALV_eNpUWtpA36lkpb0spsqqBVXjvfSFsoWpuP3pR0vO-a0WHjZVsNl-RMy7pTV999St4fH97mz8Fi-fQyv1sERcg4BlpBDJjymEVifD0EHoc60UqqVCtMVKxzLHmc5MA5aCwhihBYIrRIOWAk2JTMjne7wqjaZVvXezsaZoe4sl9xjYA4AoV3XeeVzlpvGumHDCHbB_0PeX0ktXSZ3HjTZevVXoII_O_9_Wt4wDERgn0BPTmACw</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Prado, G</creator><creator>Oliveira, M.S</creator><creator>Carvalho, E.P</creator><creator>Lima, L.C.O</creator><creator>Veloso, T</creator><creator>Souza, L.A.F</creator><creator>Cardoso, A.C.F</creator><general>Sociedade Brasileira de Ciência e Tecnologia de Alimentos</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>GPN</scope></search><sort><creationdate>20031201</creationdate><title>Ochratoxin a determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography</title><author>Prado, G ; Oliveira, M.S ; Carvalho, E.P ; Lima, L.C.O ; Veloso, T ; Souza, L.A.F ; Cardoso, A.C.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2341-fe060194635701020462f8feae9fe18e6fb1d468b0440f1d05510387f79401573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>ANALISE QUIMICA</topic><topic>beer</topic><topic>BEERS</topic><topic>BIÈRE</topic><topic>CERVEJA</topic><topic>CERVEZAS</topic><topic>CHEMICAL COMPOSITION</topic><topic>CHROMATOGRAPHIE</topic><topic>CHROMATOGRAPHY</topic><topic>CLAE</topic><topic>COMPOSICIÓN QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>CROMATOGRAFIA</topic><topic>CROMATOGRAFÍA</topic><topic>FOOD SCIENCE & TECHNOLOGY</topic><topic>HPLC</topic><topic>immunoaffinity</topic><topic>imunoafinidade</topic><topic>MICOTOXINA</topic><topic>MICOTOXINAS</topic><topic>MYCOTOXINE</topic><topic>MYCOTOXINS</topic><topic>ochratoxin A</topic><topic>OCHRATOXINE</topic><topic>OCHRATOXINS</topic><topic>OCRATOXINA</topic><topic>ocratoxina A</topic><topic>OCRATOXINAS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prado, G</creatorcontrib><creatorcontrib>Oliveira, M.S</creatorcontrib><creatorcontrib>Carvalho, E.P</creatorcontrib><creatorcontrib>Lima, L.C.O</creatorcontrib><creatorcontrib>Veloso, T</creatorcontrib><creatorcontrib>Souza, L.A.F</creatorcontrib><creatorcontrib>Cardoso, A.C.F</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>SciELO</collection><jtitle>Ciência e tecnologia de alimentos</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prado, G</au><au>Oliveira, M.S</au><au>Carvalho, E.P</au><au>Lima, L.C.O</au><au>Veloso, T</au><au>Souza, L.A.F</au><au>Cardoso, A.C.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ochratoxin a determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography</atitle><jtitle>Ciência e tecnologia de alimentos</jtitle><addtitle>Food Sci. Technol</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>23</volume><issue>suppl</issue><spage>58</spage><epage>61</epage><pages>58-61</pages><issn>0101-2061</issn><issn>1678-457X</issn><eissn>1678-457X</eissn><abstract>Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficient of variation between 0% e 11.0%. Detection limit and quantification limit were 2.0pg/mL and 8.0pg/mL, respectively. Of the total of 26 samples produced in Brazil only 6 (23%), contained trace amounts of OTA. Of the 4 imported beers, in 2, Ireland and Germany, were detected OTA at levels of 25pg/mL and 82pg/mL, respectively.
Análises de ocratoxina A em cervejas nacionais e importadas foram executadas por coluna de imunoafinidade e cromatografia líquida de alta eficiência (CLAE) com detetor de fluorescência. Recuperações de ocratoxina A em amostras de cervejas contaminadas em níveis de 8.0 a 800pg/mL, foram de 81,2% a 95%, com coeficiente de variação entre 0% e 11%. O limite de detecção e o de quantificação foram 2.0pg/mL e 8.0pg/mL, respectivamente. De um total de 26 amostras fabricadas no Brasil, somente 6 (23%) apresentavam traços (pg/mL) de ocratoxina A. Das 4 amostras de cerveja importadas analisadas, em duas, Irlanda e Alemanha, foram detectadas ocratoxina A em níveis de 25pg/mL e 82pg/mL, respectivamente.</abstract><pub>Sociedade Brasileira de Ciência e Tecnologia de Alimentos</pub><doi>10.1590/S0101-20612003000400011</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | ANALISE QUIMICA beer BEERS BIÈRE CERVEJA CERVEZAS CHEMICAL COMPOSITION CHROMATOGRAPHIE CHROMATOGRAPHY CLAE COMPOSICIÓN QUIMICA COMPOSITION CHIMIQUE CROMATOGRAFIA CROMATOGRAFÍA FOOD SCIENCE & TECHNOLOGY HPLC immunoaffinity imunoafinidade MICOTOXINA MICOTOXINAS MYCOTOXINE MYCOTOXINS ochratoxin A OCHRATOXINE OCHRATOXINS OCRATOXINA ocratoxina A OCRATOXINAS |
| title | Ochratoxin a determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography |
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