Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluatio...
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| Veröffentlicht in: | Memórias do Instituto Oswaldo Cruz 2017-11, Vol.112 (11), p.785-789 |
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| creator | Villela, Anne Drumond Rodrigues-Junior, Valnês S Pinto, Antônio Frederico Michel Falcão, Virgínia Carla de Almeida Sánchez-Quitian, Zilpa Adriana Eichler, Paula Bizarro, Cristiano Valim Basso, Luiz Augusto Santos, Diógenes Santiago |
| description | Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence. |
| doi_str_mv | 10.1590/0074-02760170105 |
| format | Article |
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An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.</description><identifier>ISSN: 0074-0276</identifier><identifier>ISSN: 1678-8060</identifier><identifier>EISSN: 1678-8060</identifier><identifier>DOI: 10.1590/0074-02760170105</identifier><identifier>PMID: 29091140</identifier><language>eng</language><publisher>Brazil: Instituto Oswaldo Cruz, Ministério da Saúde</publisher><subject>cdd gene ; cytidine deaminase ; Cytidine Deaminase - biosynthesis ; Cytidine Deaminase - genetics ; Deoxycytidine - biosynthesis ; Deoxycytidine - genetics ; Gene Knockout Techniques ; Humans ; Macrophages - microbiology ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - enzymology ; Mycobacterium tuberculosis - growth & development ; Mycobacterium tuberculosis - pathogenicity ; PARASITOLOGY ; Short Communication ; Time Factors ; TROPICAL MEDICINE</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2017-11, Vol.112 (11), p.785-789</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-5bbf9c64ca01ae759314885d5fa65a11b66ea8d6af2d8f204dee4f236daa4add3</citedby><cites>FETCH-LOGICAL-c501t-5bbf9c64ca01ae759314885d5fa65a11b66ea8d6af2d8f204dee4f236daa4add3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661903/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661903/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29091140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Villela, Anne Drumond</creatorcontrib><creatorcontrib>Rodrigues-Junior, Valnês S</creatorcontrib><creatorcontrib>Pinto, Antônio Frederico Michel</creatorcontrib><creatorcontrib>Falcão, Virgínia Carla de Almeida</creatorcontrib><creatorcontrib>Sánchez-Quitian, Zilpa Adriana</creatorcontrib><creatorcontrib>Eichler, Paula</creatorcontrib><creatorcontrib>Bizarro, Cristiano Valim</creatorcontrib><creatorcontrib>Basso, Luiz Augusto</creatorcontrib><creatorcontrib>Santos, Diógenes Santiago</creatorcontrib><title>Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages</title><title>Memórias do Instituto Oswaldo Cruz</title><addtitle>Mem Inst Oswaldo Cruz</addtitle><description>Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.</description><subject>cdd gene</subject><subject>cytidine deaminase</subject><subject>Cytidine Deaminase - biosynthesis</subject><subject>Cytidine Deaminase - genetics</subject><subject>Deoxycytidine - biosynthesis</subject><subject>Deoxycytidine - genetics</subject><subject>Gene Knockout Techniques</subject><subject>Humans</subject><subject>Macrophages - microbiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - enzymology</subject><subject>Mycobacterium tuberculosis - growth & development</subject><subject>Mycobacterium tuberculosis - pathogenicity</subject><subject>PARASITOLOGY</subject><subject>Short Communication</subject><subject>Time Factors</subject><subject>TROPICAL MEDICINE</subject><issn>0074-0276</issn><issn>1678-8060</issn><issn>1678-8060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNpVUsGO0zAQjRCILQt3TihHLl1mEttJLkioWtiVFnEAztbEHrfppnGxk6L9e5xtt2Llg0dv5r15epose49whbKBTwCVWEJRKcAKEOSLbIGqqpc1KHiZLc7ti-xNjFtIZanE6-yiaKBBFLDI-pUf4hgmM3Z-yL3Lvz8Y35IZOXTTLh-nloOZeh-7mBtr8_vBm3s_jTkNNucD9RM9MbvhQHGu59Y6-L_jJmH5jkzw-w2tOb7NXjnqI787_ZfZ76_Xv1Y3y7sf325XX-6WRgKOS9m2rjFKGAIkrmRToqhraaUjJQmxVYqptopcYWtXgLDMwhWlskSCrC0vs9ujrvW01fvQ7Sg8aE-dfgR8WGsKY2d61qRq5xo0wI4F1VVLhRQtKWRRCmSVtK6OWtF03Hu99VMYknn9c05Xz-kWc_qAmIBaJsLnI2E_tTu2hocxUP_MxfPO0G302h-0VAobKJPAx5NA8H8mjqPeddFw39PAfooaG1lLkR6mUTiOpohjDOzOaxD0fCH6bPJ0IYny4X97Z8LTSZT_AGeZt4A</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Villela, Anne Drumond</creator><creator>Rodrigues-Junior, Valnês S</creator><creator>Pinto, Antônio Frederico Michel</creator><creator>Falcão, Virgínia Carla de Almeida</creator><creator>Sánchez-Quitian, Zilpa Adriana</creator><creator>Eichler, Paula</creator><creator>Bizarro, Cristiano Valim</creator><creator>Basso, Luiz Augusto</creator><creator>Santos, Diógenes Santiago</creator><general>Instituto Oswaldo Cruz, Ministério da Saúde</general><general>Fundação Oswaldo Cruz (FIOCRUZ)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>201711</creationdate><title>Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages</title><author>Villela, Anne Drumond ; Rodrigues-Junior, Valnês S ; Pinto, Antônio Frederico Michel ; Falcão, Virgínia Carla de Almeida ; Sánchez-Quitian, Zilpa Adriana ; Eichler, Paula ; Bizarro, Cristiano Valim ; Basso, Luiz Augusto ; Santos, Diógenes Santiago</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-5bbf9c64ca01ae759314885d5fa65a11b66ea8d6af2d8f204dee4f236daa4add3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>cdd gene</topic><topic>cytidine deaminase</topic><topic>Cytidine Deaminase - biosynthesis</topic><topic>Cytidine Deaminase - genetics</topic><topic>Deoxycytidine - biosynthesis</topic><topic>Deoxycytidine - genetics</topic><topic>Gene Knockout Techniques</topic><topic>Humans</topic><topic>Macrophages - microbiology</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - enzymology</topic><topic>Mycobacterium tuberculosis - growth & development</topic><topic>Mycobacterium tuberculosis - pathogenicity</topic><topic>PARASITOLOGY</topic><topic>Short Communication</topic><topic>Time Factors</topic><topic>TROPICAL MEDICINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Villela, Anne Drumond</creatorcontrib><creatorcontrib>Rodrigues-Junior, Valnês S</creatorcontrib><creatorcontrib>Pinto, Antônio Frederico Michel</creatorcontrib><creatorcontrib>Falcão, Virgínia Carla de Almeida</creatorcontrib><creatorcontrib>Sánchez-Quitian, Zilpa Adriana</creatorcontrib><creatorcontrib>Eichler, Paula</creatorcontrib><creatorcontrib>Bizarro, Cristiano Valim</creatorcontrib><creatorcontrib>Basso, Luiz Augusto</creatorcontrib><creatorcontrib>Santos, Diógenes Santiago</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Villela, Anne Drumond</au><au>Rodrigues-Junior, Valnês S</au><au>Pinto, Antônio Frederico Michel</au><au>Falcão, Virgínia Carla de Almeida</au><au>Sánchez-Quitian, Zilpa Adriana</au><au>Eichler, Paula</au><au>Bizarro, Cristiano Valim</au><au>Basso, Luiz Augusto</au><au>Santos, Diógenes Santiago</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages</atitle><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle><addtitle>Mem Inst Oswaldo Cruz</addtitle><date>2017-11</date><risdate>2017</risdate><volume>112</volume><issue>11</issue><spage>785</spage><epage>789</epage><pages>785-789</pages><issn>0074-0276</issn><issn>1678-8060</issn><eissn>1678-8060</eissn><abstract>Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.</abstract><cop>Brazil</cop><pub>Instituto Oswaldo Cruz, Ministério da Saúde</pub><pmid>29091140</pmid><doi>10.1590/0074-02760170105</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | cdd gene cytidine deaminase Cytidine Deaminase - biosynthesis Cytidine Deaminase - genetics Deoxycytidine - biosynthesis Deoxycytidine - genetics Gene Knockout Techniques Humans Macrophages - microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - enzymology Mycobacterium tuberculosis - growth & development Mycobacterium tuberculosis - pathogenicity PARASITOLOGY Short Communication Time Factors TROPICAL MEDICINE |
| title | Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
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