Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden
Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into...
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| Veröffentlicht in: | Memórias do Instituto Oswaldo Cruz 2007-09, Vol.102 (6), p.725-731 |
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| creator | Gomes, Andréia Bartachini Soares, Killarney Ataíde Bueno, Ednéia Casagrande Espindola, Noeli Maria Iha, Alberto Hiroshi Maia, Antônio Augusto Mendes Peralta, Regina Helena Saramargo Vaz, Adelaide José |
| description | Seven swine were experimentally infected with Taenia solium eggs and
blood samples from each animal were periodically collected. At the end
of the experiment (t140) the animals did not show clinical aspects of
cysticercosis or parasites in tongue inspection. All animals were
slaughtered and cut into thin slices in searching for cysts. The number
of cysts found in each animal varied from 1 to 85. Enzyme-linked
immunosorbent assay (ELISA) tests for antibody (Ab) detection and for
antigen (Ag) detection were performed, which presented respectively 71
and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps
Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as
Ag, five (71%) and six (86%) animals were positive, respectively. The
association between Ag-ELISA with any IB (18/14 or LLGP) allowed the
detection of all animals at 140 days post-experimental infection (days
p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the
detection of all animals since 70 days p.e.i., and the association
between IB LLGP and Ag-ELISA allowed the detection of all animals since
112 days p.e.i. While all animals could be considered healthy by
conventional screening tests, the use of immunoassays for detecting Ab
and Ag showed better accuracy; therefore it would be more useful than
usual clinical examination for screening cysticercosis in slightly
infected pigs. |
| doi_str_mv | 10.1590/S0074-02762007005000085 |
| format | Article |
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blood samples from each animal were periodically collected. At the end
of the experiment (t140) the animals did not show clinical aspects of
cysticercosis or parasites in tongue inspection. All animals were
slaughtered and cut into thin slices in searching for cysts. The number
of cysts found in each animal varied from 1 to 85. Enzyme-linked
immunosorbent assay (ELISA) tests for antibody (Ab) detection and for
antigen (Ag) detection were performed, which presented respectively 71
and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps
Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as
Ag, five (71%) and six (86%) animals were positive, respectively. The
association between Ag-ELISA with any IB (18/14 or LLGP) allowed the
detection of all animals at 140 days post-experimental infection (days
p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the
detection of all animals since 70 days p.e.i., and the association
between IB LLGP and Ag-ELISA allowed the detection of all animals since
112 days p.e.i. While all animals could be considered healthy by
conventional screening tests, the use of immunoassays for detecting Ab
and Ag showed better accuracy; therefore it would be more useful than
usual clinical examination for screening cysticercosis in slightly
infected pigs.</description><identifier>ISSN: 1678-8060</identifier><identifier>ISSN: 0074-0276</identifier><identifier>EISSN: 1678-8060</identifier><identifier>DOI: 10.1590/S0074-02762007005000085</identifier><identifier>PMID: 17924002</identifier><language>eng</language><publisher>Brazil: Fundação Oswaldo Cruz, Fiocruz</publisher><subject>Animals ; Antibodies, Helminth - blood ; Antigens, Helminth - blood ; Cysticercosis - diagnosis ; Cysticercosis - immunology ; Cysticercosis - parasitology ; Cysticercosis - veterinary ; Enzyme-Linked Immunosorbent Assay ; Immunoblotting ; PARASITOLOGY ; Reproducibility of Results ; Sensitivity and Specificity ; Swine ; swine cysticercosis - larval antigens - antibodies - enzyme-linked immunosorbent assay - immunoblot ; Swine Diseases - diagnosis ; Swine Diseases - immunology ; Swine Diseases - parasitology ; Taenia solium - immunology ; Taenia solium - isolation & purification ; TROPICAL MEDICINE</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2007-09, Vol.102 (6), p.725-731</ispartof><rights>Copyright 2007 Instituto Oswaldo Cruz - Fiocruz</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b438t-8caf8fbbfebefc18ee937ee87ad3d459f1ac55955da1260fcea153600490b72e3</citedby><cites>FETCH-LOGICAL-b438t-8caf8fbbfebefc18ee937ee87ad3d459f1ac55955da1260fcea153600490b72e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906,79175</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17924002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gomes, Andréia Bartachini</creatorcontrib><creatorcontrib>Soares, Killarney Ataíde</creatorcontrib><creatorcontrib>Bueno, Ednéia Casagrande</creatorcontrib><creatorcontrib>Espindola, Noeli Maria</creatorcontrib><creatorcontrib>Iha, Alberto Hiroshi</creatorcontrib><creatorcontrib>Maia, Antônio Augusto Mendes</creatorcontrib><creatorcontrib>Peralta, Regina Helena Saramargo</creatorcontrib><creatorcontrib>Vaz, Adelaide José</creatorcontrib><title>Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden</title><title>Memórias do Instituto Oswaldo Cruz</title><addtitle>Mem Inst Oswaldo Cruz</addtitle><description>Seven swine were experimentally infected with Taenia solium eggs and
blood samples from each animal were periodically collected. At the end
of the experiment (t140) the animals did not show clinical aspects of
cysticercosis or parasites in tongue inspection. All animals were
slaughtered and cut into thin slices in searching for cysts. The number
of cysts found in each animal varied from 1 to 85. Enzyme-linked
immunosorbent assay (ELISA) tests for antibody (Ab) detection and for
antigen (Ag) detection were performed, which presented respectively 71
and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps
Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as
Ag, five (71%) and six (86%) animals were positive, respectively. The
association between Ag-ELISA with any IB (18/14 or LLGP) allowed the
detection of all animals at 140 days post-experimental infection (days
p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the
detection of all animals since 70 days p.e.i., and the association
between IB LLGP and Ag-ELISA allowed the detection of all animals since
112 days p.e.i. While all animals could be considered healthy by
conventional screening tests, the use of immunoassays for detecting Ab
and Ag showed better accuracy; therefore it would be more useful than
usual clinical examination for screening cysticercosis in slightly
infected pigs.</description><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antigens, Helminth - blood</subject><subject>Cysticercosis - diagnosis</subject><subject>Cysticercosis - immunology</subject><subject>Cysticercosis - parasitology</subject><subject>Cysticercosis - veterinary</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Immunoblotting</subject><subject>PARASITOLOGY</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Swine</subject><subject>swine cysticercosis - larval antigens - antibodies - enzyme-linked immunosorbent assay - immunoblot</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - parasitology</subject><subject>Taenia solium - immunology</subject><subject>Taenia solium - isolation & purification</subject><subject>TROPICAL MEDICINE</subject><issn>1678-8060</issn><issn>0074-0276</issn><issn>1678-8060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><sourceid>EIF</sourceid><recordid>eNplUU1v3CAURFWr5qP9Cy2n3pw-jDH4WK36ESlSD03PCOOHQmTDFnBWe84fL-luupWKkBjQzBs0Q8h7BldMDPDxB4DsGmhl31YEIKAuJV6Qc9ZL1Sjo4eU_-Ixc5HwPlc_77jU5Y3Jou3o9J4-buGxNMsU_IMUHM68VxkCjo5N3DhOGQv2yrCGanM0-UxcTLXdIJyxon7m3BoM3NMfZrwu1-1y8xWRj9pn6QPPOB6Q7X-7oHHf0yTD7gnRc04ThDXnlzJzx7fG8JD-_fL7dfGtuvn-93ny6acaOq9Ioa5xy4-hwRGeZQhy4RFTSTHzqxOCYsUIMQkyGtT04i4YJ3gN0A4yyRX5Jrg5zs_U4R30f1xSqof6Tpj6lWRMDYKwKPhwE2xR_rZiLXny2OM8mYFyz7hXvFQNeifJAtCnmnNDpbfKLSXvNQD8V9p_FsbCqfHe0WMcFp5Pu2NDp06Ov4Qb8y7DJG_38GG3dIBnr-W9X76NX</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Gomes, Andréia Bartachini</creator><creator>Soares, Killarney Ataíde</creator><creator>Bueno, Ednéia Casagrande</creator><creator>Espindola, Noeli Maria</creator><creator>Iha, Alberto Hiroshi</creator><creator>Maia, Antônio Augusto Mendes</creator><creator>Peralta, Regina Helena Saramargo</creator><creator>Vaz, Adelaide José</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><general>Instituto Oswaldo Cruz, Ministério da Saúde</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>20070901</creationdate><title>Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden</title><author>Gomes, Andréia Bartachini ; Soares, Killarney Ataíde ; Bueno, Ednéia Casagrande ; Espindola, Noeli Maria ; Iha, Alberto Hiroshi ; Maia, Antônio Augusto Mendes ; Peralta, Regina Helena Saramargo ; Vaz, Adelaide José</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b438t-8caf8fbbfebefc18ee937ee87ad3d459f1ac55955da1260fcea153600490b72e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antigens, Helminth - blood</topic><topic>Cysticercosis - diagnosis</topic><topic>Cysticercosis - immunology</topic><topic>Cysticercosis - parasitology</topic><topic>Cysticercosis - veterinary</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Immunoblotting</topic><topic>PARASITOLOGY</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Swine</topic><topic>swine cysticercosis - larval antigens - antibodies - enzyme-linked immunosorbent assay - immunoblot</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - parasitology</topic><topic>Taenia solium - immunology</topic><topic>Taenia solium - isolation & purification</topic><topic>TROPICAL MEDICINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gomes, Andréia Bartachini</creatorcontrib><creatorcontrib>Soares, Killarney Ataíde</creatorcontrib><creatorcontrib>Bueno, Ednéia Casagrande</creatorcontrib><creatorcontrib>Espindola, Noeli Maria</creatorcontrib><creatorcontrib>Iha, Alberto Hiroshi</creatorcontrib><creatorcontrib>Maia, Antônio Augusto Mendes</creatorcontrib><creatorcontrib>Peralta, Regina Helena Saramargo</creatorcontrib><creatorcontrib>Vaz, Adelaide José</creatorcontrib><collection>Bioline International</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gomes, Andréia Bartachini</au><au>Soares, Killarney Ataíde</au><au>Bueno, Ednéia Casagrande</au><au>Espindola, Noeli Maria</au><au>Iha, Alberto Hiroshi</au><au>Maia, Antônio Augusto Mendes</au><au>Peralta, Regina Helena Saramargo</au><au>Vaz, Adelaide José</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden</atitle><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle><addtitle>Mem Inst Oswaldo Cruz</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>102</volume><issue>6</issue><spage>725</spage><epage>731</epage><pages>725-731</pages><issn>1678-8060</issn><issn>0074-0276</issn><eissn>1678-8060</eissn><abstract>Seven swine were experimentally infected with Taenia solium eggs and
blood samples from each animal were periodically collected. At the end
of the experiment (t140) the animals did not show clinical aspects of
cysticercosis or parasites in tongue inspection. All animals were
slaughtered and cut into thin slices in searching for cysts. The number
of cysts found in each animal varied from 1 to 85. Enzyme-linked
immunosorbent assay (ELISA) tests for antibody (Ab) detection and for
antigen (Ag) detection were performed, which presented respectively 71
and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps
Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as
Ag, five (71%) and six (86%) animals were positive, respectively. The
association between Ag-ELISA with any IB (18/14 or LLGP) allowed the
detection of all animals at 140 days post-experimental infection (days
p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the
detection of all animals since 70 days p.e.i., and the association
between IB LLGP and Ag-ELISA allowed the detection of all animals since
112 days p.e.i. While all animals could be considered healthy by
conventional screening tests, the use of immunoassays for detecting Ab
and Ag showed better accuracy; therefore it would be more useful than
usual clinical examination for screening cysticercosis in slightly
infected pigs.</abstract><cop>Brazil</cop><pub>Fundação Oswaldo Cruz, Fiocruz</pub><pmid>17924002</pmid><doi>10.1590/S0074-02762007005000085</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1678-8060 |
| ispartof | Memórias do Instituto Oswaldo Cruz, 2007-09, Vol.102 (6), p.725-731 |
| issn | 1678-8060 0074-0276 1678-8060 |
| language | eng |
| recordid | cdi_scielo_journals_S0074_02762007000600011 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Bioline International |
| subjects | Animals Antibodies, Helminth - blood Antigens, Helminth - blood Cysticercosis - diagnosis Cysticercosis - immunology Cysticercosis - parasitology Cysticercosis - veterinary Enzyme-Linked Immunosorbent Assay Immunoblotting PARASITOLOGY Reproducibility of Results Sensitivity and Specificity Swine swine cysticercosis - larval antigens - antibodies - enzyme-linked immunosorbent assay - immunoblot Swine Diseases - diagnosis Swine Diseases - immunology Swine Diseases - parasitology Taenia solium - immunology Taenia solium - isolation & purification TROPICAL MEDICINE |
| title | Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden |
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