Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance
The present study investigated if hepatitis B virus (HBV) mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers...
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| Veröffentlicht in: | Revista do Instituto de Medicina Tropical de São Paulo 2010-01, Vol.52 (1), p.25-30 |
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| creator | Bertolini, Dennis Armando Ribeiro, Patrícia Cristiane Lemos, Marcílio Figueiredo Saraceni, Cláudia Patara Pinho, João Renato Rebello |
| description | The present study investigated if hepatitis B virus (HBV) mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed. |
| doi_str_mv | 10.1590/s0036-46652010000100004 |
| format | Article |
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Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.</description><identifier>ISSN: 0036-4665</identifier><identifier>ISSN: 1678-9946</identifier><identifier>EISSN: 1678-9946</identifier><identifier>EISSN: 0036-4665</identifier><identifier>DOI: 10.1590/s0036-46652010000100004</identifier><identifier>PMID: 20305951</identifier><identifier>CODEN: RMTSAE</identifier><language>eng</language><publisher>Brazil: Instituto de Medicina Tropical de Sao Paulo</publisher><subject>Base Sequence ; Brazil ; Child ; DNA, Viral - genetics ; Genotype ; Hepatitis B - diagnosis ; Hepatitis B - immunology ; Hepatitis B - virology ; Hepatitis B Antibodies - blood ; Hepatitis B Core Antigens - blood ; Hepatitis B Surface Antigens - blood ; Hepatitis B Vaccines - immunology ; Hepatitis B virus - genetics ; Hepatitis B virus - immunology ; Humans ; Molecular Sequence Data ; Mutation - genetics ; Polymerase Chain Reaction ; TROPICAL MEDICINE</subject><ispartof>Revista do Instituto de Medicina Tropical de São Paulo, 2010-01, Vol.52 (1), p.25-30</ispartof><rights>Copyright Instituto de Medicina Tropical de Sao Paulo Jan/Feb 2010</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-feae9cc838bffa1435c8ac3abdde101752ce71199b95ad2bbcf2ef471c3099a3</citedby><cites>FETCH-LOGICAL-c427t-feae9cc838bffa1435c8ac3abdde101752ce71199b95ad2bbcf2ef471c3099a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,860,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20305951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bertolini, Dennis Armando</creatorcontrib><creatorcontrib>Ribeiro, Patrícia Cristiane</creatorcontrib><creatorcontrib>Lemos, Marcílio Figueiredo</creatorcontrib><creatorcontrib>Saraceni, Cláudia Patara</creatorcontrib><creatorcontrib>Pinho, João Renato Rebello</creatorcontrib><title>Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance</title><title>Revista do Instituto de Medicina Tropical de São Paulo</title><addtitle>Rev Inst Med Trop Sao Paulo</addtitle><description>The present study investigated if hepatitis B virus (HBV) mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.</description><subject>Base Sequence</subject><subject>Brazil</subject><subject>Child</subject><subject>DNA, Viral - genetics</subject><subject>Genotype</subject><subject>Hepatitis B - diagnosis</subject><subject>Hepatitis B - immunology</subject><subject>Hepatitis B - virology</subject><subject>Hepatitis B Antibodies - blood</subject><subject>Hepatitis B Core Antigens - blood</subject><subject>Hepatitis B Surface Antigens - blood</subject><subject>Hepatitis B Vaccines - immunology</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - immunology</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Mutation - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>TROPICAL MEDICINE</subject><issn>0036-4665</issn><issn>1678-9946</issn><issn>1678-9946</issn><issn>0036-4665</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kdFu0zAUhi0EYt3gFcDiZjfLsOM4ri9pBRRpEgh2H504J9RT6hQfZ9P2GjwBz7IXw11HL0DCsnxk6_t_2_9h7LUU51Jb8ZaEUHVR1bUuhRR5PCzVEzaTtZkX1lb1UzY7QEfsmOgqE1bY-jk7KoUS2mo5Yz-Xa4jgEkZ_B8mPgY89B77Cbd4lT3zBr32ciFOK4APPk8YprW-QsibwL1kd7n-d8UWEOz-c8W1EwpB8-M43U3qwpN3htR8nGm45EI3OQ8KO3_i05pDZYrUg_hXJU4Lg8AV71sNA-PKxnrDLD-8vl6vi4vPHT8t3F4WrSpOKHgGtc3M1b_seZKW0m4NT0HYdSiGNLh0aKa1trYaubFvXl9hXRjolrAV1ws73tuQ8DmNzNU4x5Puab7vcmn_CzYLTvWAbxx9TDqDZeHI4DBAwf64xShlV1aXJ5Ju_yIO5tLWWVtdlhswecnEkitg32-g3EG8bKZpdl__zkFeP9lO7we6g-9NW9Rs9cKQ1</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Bertolini, Dennis Armando</creator><creator>Ribeiro, Patrícia Cristiane</creator><creator>Lemos, Marcílio Figueiredo</creator><creator>Saraceni, Cláudia Patara</creator><creator>Pinho, João Renato Rebello</creator><general>Instituto de Medicina Tropical de Sao Paulo</general><general>Instituto de Medicina Tropical</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CLZPN</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>20100101</creationdate><title>Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance</title><author>Bertolini, Dennis Armando ; Ribeiro, Patrícia Cristiane ; Lemos, Marcílio Figueiredo ; Saraceni, Cláudia Patara ; Pinho, João Renato Rebello</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-feae9cc838bffa1435c8ac3abdde101752ce71199b95ad2bbcf2ef471c3099a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Base Sequence</topic><topic>Brazil</topic><topic>Child</topic><topic>DNA, Viral - 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Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.</abstract><cop>Brazil</cop><pub>Instituto de Medicina Tropical de Sao Paulo</pub><pmid>20305951</pmid><doi>10.1590/s0036-46652010000100004</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Revista do Instituto de Medicina Tropical de São Paulo, 2010-01, Vol.52 (1), p.25-30 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Base Sequence Brazil Child DNA, Viral - genetics Genotype Hepatitis B - diagnosis Hepatitis B - immunology Hepatitis B - virology Hepatitis B Antibodies - blood Hepatitis B Core Antigens - blood Hepatitis B Surface Antigens - blood Hepatitis B Vaccines - immunology Hepatitis B virus - genetics Hepatitis B virus - immunology Humans Molecular Sequence Data Mutation - genetics Polymerase Chain Reaction TROPICAL MEDICINE |
| title | Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance |
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