SHV-type extended-spectrum beta-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico
In this work we report the molecular characterization of beta-lactam antibiotics resistance conferred by genes contained in plasmids from enterobacteria producing extended-spectrum beta-lactamases (ESBL). Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven...
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| Veröffentlicht in: | Salud pública de México 2007-12, Vol.49 (6), p.415-421 |
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| creator | Garza-Ramos, Ulises Martínez-Romero, Esperanza Silva-Sánchez, Jesús |
| description | In this work we report the molecular characterization of beta-lactam antibiotics resistance conferred by genes contained in plasmids from enterobacteria producing extended-spectrum beta-lactamases (ESBL).
Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven different hospitals in Mexico during 1990-1992 and 1996-1998 were analyzed at the Bacterial Resistance Laboratory (National Institute Public Health, Cuernavaca). Molecular characterization included PFGE, IEF of beta-lactamases, bacterial conjugation, PCR amplification and DNA sequencing, plasmid extraction and restriction.
Isolates were genetically unrelated. ESBL identified were SHV-2 (5/14) and SHV-5 (9/14) type. Cephalosporin-resistance was transferable in 9 of 14 (64%) clinical isolates with only one conjugative plasmid, DNA finger printing showed a similar band pattern in plasmids.
The dissemination of cephalosporin resistance was due to related plasmids carrying the ESBL genes. |
| doi_str_mv | 10.1590/S0036-36342007000600008 |
| format | Article |
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Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven different hospitals in Mexico during 1990-1992 and 1996-1998 were analyzed at the Bacterial Resistance Laboratory (National Institute Public Health, Cuernavaca). Molecular characterization included PFGE, IEF of beta-lactamases, bacterial conjugation, PCR amplification and DNA sequencing, plasmid extraction and restriction.
Isolates were genetically unrelated. ESBL identified were SHV-2 (5/14) and SHV-5 (9/14) type. Cephalosporin-resistance was transferable in 9 of 14 (64%) clinical isolates with only one conjugative plasmid, DNA finger printing showed a similar band pattern in plasmids.
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Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven different hospitals in Mexico during 1990-1992 and 1996-1998 were analyzed at the Bacterial Resistance Laboratory (National Institute Public Health, Cuernavaca). Molecular characterization included PFGE, IEF of beta-lactamases, bacterial conjugation, PCR amplification and DNA sequencing, plasmid extraction and restriction.
Isolates were genetically unrelated. ESBL identified were SHV-2 (5/14) and SHV-5 (9/14) type. Cephalosporin-resistance was transferable in 9 of 14 (64%) clinical isolates with only one conjugative plasmid, DNA finger printing showed a similar band pattern in plasmids.
The dissemination of cephalosporin resistance was due to related plasmids carrying the ESBL genes.</description><subject>Bacterial Proteins - classification</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>beta-Lactam Resistance - genetics</subject><subject>beta-Lactamases - classification</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - isolation & purification</subject><subject>Cross Infection - epidemiology</subject><subject>Cross Infection - microbiology</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins - classification</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia coli Proteins - isolation & purification</subject><subject>Health Policy & Services</subject><subject>Humans</subject><subject>Klebsiella - drug effects</subject><subject>Klebsiella - enzymology</subject><subject>Klebsiella - genetics</subject><subject>Klebsiella Infections - epidemiology</subject><subject>Klebsiella Infections - microbiology</subject><subject>Mexico - epidemiology</subject><subject>R Factors - genetics</subject><issn>0036-3634</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFP3DAQhX2gAgr8BfCpag9ZJrFjJ8cWQbfSIg4LXKNZZyIZOXFqJxJc-eU1bNQeKnEYzUjzvXnSG8YucljlZQ2XWwChMqGELAA0AKhUUB2w47-LI_Y5xieAQhRCH7KjvMq10pAfs9ft-jGbXkbi9DzR0FKbxZHMFOae72jCzKGZsMdI_Ov19sfmG8eQ2MH4hHI78EAOpzSODmNv28i74PsETBT8LmkpWOTG2cEadNxG_4Yv1C09W-NP2acOXaSzpZ-wh5vr-6t1trn7-evq-yYzIpdVpjusSxBQCglyV6iiULoqOlMT1a0Q2pi2RlS67WQpUSpACYSqTFAltSRxwlb7u9FYcr558nMYkmHzHmDzX4BJ8GUvGIP_PVOcmt5GQ87hQH6OjaqhFroWCdR70AQfY6CuGYPtMbw0OTRvP_rA4nyxmHc9tf90y4PEH64HjE8</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Garza-Ramos, Ulises</creator><creator>Martínez-Romero, Esperanza</creator><creator>Silva-Sánchez, Jesús</creator><general>Instituto Nacional de Salud Pública</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>20071201</creationdate><title>SHV-type extended-spectrum beta-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico</title><author>Garza-Ramos, Ulises ; Martínez-Romero, Esperanza ; Silva-Sánchez, Jesús</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3148-7fa9503053404b26226782fc9ee9d337ccd9aa67df454a460a40ea657828474e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Bacterial Proteins - classification</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>beta-Lactam Resistance - genetics</topic><topic>beta-Lactamases - classification</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - isolation & purification</topic><topic>Cross Infection - epidemiology</topic><topic>Cross Infection - microbiology</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Proteins - classification</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Escherichia coli Proteins - isolation & purification</topic><topic>Health Policy & Services</topic><topic>Humans</topic><topic>Klebsiella - drug effects</topic><topic>Klebsiella - enzymology</topic><topic>Klebsiella - genetics</topic><topic>Klebsiella Infections - epidemiology</topic><topic>Klebsiella Infections - microbiology</topic><topic>Mexico - epidemiology</topic><topic>R Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garza-Ramos, Ulises</creatorcontrib><creatorcontrib>Martínez-Romero, Esperanza</creatorcontrib><creatorcontrib>Silva-Sánchez, Jesús</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Salud pública de México</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garza-Ramos, Ulises</au><au>Martínez-Romero, Esperanza</au><au>Silva-Sánchez, Jesús</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SHV-type extended-spectrum beta-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico</atitle><jtitle>Salud pública de México</jtitle><addtitle>Salud Publica Mex</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>49</volume><issue>6</issue><spage>415</spage><epage>421</epage><pages>415-421</pages><issn>0036-3634</issn><abstract>In this work we report the molecular characterization of beta-lactam antibiotics resistance conferred by genes contained in plasmids from enterobacteria producing extended-spectrum beta-lactamases (ESBL).
Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven different hospitals in Mexico during 1990-1992 and 1996-1998 were analyzed at the Bacterial Resistance Laboratory (National Institute Public Health, Cuernavaca). Molecular characterization included PFGE, IEF of beta-lactamases, bacterial conjugation, PCR amplification and DNA sequencing, plasmid extraction and restriction.
Isolates were genetically unrelated. ESBL identified were SHV-2 (5/14) and SHV-5 (9/14) type. Cephalosporin-resistance was transferable in 9 of 14 (64%) clinical isolates with only one conjugative plasmid, DNA finger printing showed a similar band pattern in plasmids.
The dissemination of cephalosporin resistance was due to related plasmids carrying the ESBL genes.</abstract><cop>Mexico</cop><pub>Instituto Nacional de Salud Pública</pub><pmid>18176701</pmid><doi>10.1590/S0036-36342007000600008</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Business Source Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Bacterial Proteins - classification Bacterial Proteins - genetics Bacterial Proteins - isolation & purification beta-Lactam Resistance - genetics beta-Lactamases - classification beta-Lactamases - genetics beta-Lactamases - isolation & purification Cross Infection - epidemiology Cross Infection - microbiology DNA Fingerprinting DNA, Bacterial - genetics Drug Resistance, Multiple, Bacterial - genetics Escherichia coli - drug effects Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Escherichia coli Proteins - classification Escherichia coli Proteins - genetics Escherichia coli Proteins - isolation & purification Health Policy & Services Humans Klebsiella - drug effects Klebsiella - enzymology Klebsiella - genetics Klebsiella Infections - epidemiology Klebsiella Infections - microbiology Mexico - epidemiology R Factors - genetics |
| title | SHV-type extended-spectrum beta-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico |
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