Carnitine analysis in pterygium

Carnitine analysis in pterygium

ABSTRACT Purpose: The aim of the present study was to measure the free carnitine and acylcarnitine levels in pterygium tissue and normal conjunctival tissue at the metabolomics level using tandem mass spectrometry. Methods: In this prospective, clinical randomized study, pterygium tissues and normal...

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Veröffentlicht in:Arquivos brasileiros de oftalmologia 2020-02, Vol.83 (1), p.5-10
Hauptverfasser: Saglik, Ayhan, Koyuncu, Ismail, Yalcin, Hamza, Adibelli, Fatih Mehmet, Gonel, Ataman, Toptan, Muslum
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OPHTHALMOLOGY
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container_title Arquivos brasileiros de oftalmologia
container_volume 83
creator Saglik, Ayhan
Koyuncu, Ismail
Yalcin, Hamza
Adibelli, Fatih Mehmet
Gonel, Ataman
Toptan, Muslum
description ABSTRACT Purpose: The aim of the present study was to measure the free carnitine and acylcarnitine levels in pterygium tissue and normal conjunctival tissue at the metabolomics level using tandem mass spectrometry. Methods: In this prospective, clinical randomized study, pterygium tissues and normal conjunctival tissues taken during pterygium excision with autograft were compared regarding their free carnitine and acylcarnitine profiles. After tissue homogenization, carnitine levels were measured using tandem mass spectrometry. The data were statistically analyzed with the Wilcoxon signed-rank test. Results: Pterygium and normal conjunctival tissue samples from a single eye of 29 patients (16 females, 13 males; mean age, 54.75 ± 11.25 years [range, 21-78 years]) were evaluated. While the free carnitine (C0) level was significantly high in the pterygium tissue (p
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Methods: In this prospective, clinical randomized study, pterygium tissues and normal conjunctival tissues taken during pterygium excision with autograft were compared regarding their free carnitine and acylcarnitine profiles. After tissue homogenization, carnitine levels were measured using tandem mass spectrometry. The data were statistically analyzed with the Wilcoxon signed-rank test. Results: Pterygium and normal conjunctival tissue samples from a single eye of 29 patients (16 females, 13 males; mean age, 54.75 ± 11.25 years [range, 21-78 years]) were evaluated. While the free carnitine (C0) level was significantly high in the pterygium tissue (p&lt;0.001), acylcarnitine levels were significantly high in some esterized derivatives (C2, C5, C5:1, C5DC, C16:1, C18, methylglutarylcarnitine) (p&lt;0.05). No statistically significant difference was determined for the other esterized derivatives (p&gt;0.05). Conclusion: That the carnitine levels in pterygium tissue were higher suggests that acceleration of cell metabolism developed secondary to chronic inflammation and the premalignant characteristics of pterygium tissue. High carnitine levels may also effectively suppress the apoptosis process. The data reported in our study indicate that further, more extensive studies of the carnitine profile could help clarify the pathogenesis of pterygium.</description><identifier>ISSN: 1678-2925</identifier><identifier>DOI: 10.5935/0004-2749.20200001</identifier><language>por</language><publisher>Conselho Brasileiro de Oftalmologia</publisher><subject>OPHTHALMOLOGY</subject><ispartof>Arquivos brasileiros de oftalmologia, 2020-02, Vol.83 (1), p.5-10</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids></links><search><creatorcontrib>Saglik, Ayhan</creatorcontrib><creatorcontrib>Koyuncu, Ismail</creatorcontrib><creatorcontrib>Yalcin, Hamza</creatorcontrib><creatorcontrib>Adibelli, Fatih Mehmet</creatorcontrib><creatorcontrib>Gonel, Ataman</creatorcontrib><creatorcontrib>Toptan, Muslum</creatorcontrib><title>Carnitine analysis in pterygium</title><title>Arquivos brasileiros de oftalmologia</title><addtitle>Arq. 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While the free carnitine (C0) level was significantly high in the pterygium tissue (p&lt;0.001), acylcarnitine levels were significantly high in some esterized derivatives (C2, C5, C5:1, C5DC, C16:1, C18, methylglutarylcarnitine) (p&lt;0.05). No statistically significant difference was determined for the other esterized derivatives (p&gt;0.05). Conclusion: That the carnitine levels in pterygium tissue were higher suggests that acceleration of cell metabolism developed secondary to chronic inflammation and the premalignant characteristics of pterygium tissue. High carnitine levels may also effectively suppress the apoptosis process. 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title Carnitine analysis in pterygium
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