Investigating the effect of intracameral cefuroxime on oxidative stress and apoptosis in the rat cornea
We examined the effect of intracameral administration of cefuroxime on oxidative stress and endothelial apoptosis in rat corneal tissue. In total, 30 rats were divided into 3 groups of 10 rats each (intracameral administration of cefuroxime 0.1 mg/0.01 mL (cefuroxime group); intracameral administrat...
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| Veröffentlicht in: | Arquivos brasileiros de oftalmologia 2019-07, Vol.82 (4), p.322-328 |
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| creator | Ozkan, Ufuk Akal, Ali Ozkan, Kudret Yilmaz, Omer Faruk Adıbelli, Fatih Mehmet |
| description | We examined the effect of intracameral administration of cefuroxime on oxidative stress and endothelial apoptosis in rat corneal tissue.
In total, 30 rats were divided into 3 groups of 10 rats each (intracameral administration of cefuroxime 0.1 mg/0.01 mL (cefuroxime group); intracameral administration of balanced salt solution 0.01 mL (control group); or absence of intracameral injection (sham group). Corneal endothelial apoptosis was assessed by immunohistochemical analysis using caspase-3 and caspase-8. Total oxidant status, total antioxidant status, oxidative stress index, and paraoxonase and arylesterase levels were examined in corneal endothelial tissue and serum.
Paraoxonase levels in the serum were significantly different between the sham and cefuroxime groups (p=0.027). A significant difference was also observed in total oxidant status levels between the cefuroxime and balanced salt solution groups (p=0.023). In addition, there were significant differences in total antioxidant status levels in corneal tissue between the cefuroxime and sham groups (p |
| doi_str_mv | 10.5935/0004-2749.20190067 |
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In total, 30 rats were divided into 3 groups of 10 rats each (intracameral administration of cefuroxime 0.1 mg/0.01 mL (cefuroxime group); intracameral administration of balanced salt solution 0.01 mL (control group); or absence of intracameral injection (sham group). Corneal endothelial apoptosis was assessed by immunohistochemical analysis using caspase-3 and caspase-8. Total oxidant status, total antioxidant status, oxidative stress index, and paraoxonase and arylesterase levels were examined in corneal endothelial tissue and serum.
Paraoxonase levels in the serum were significantly different between the sham and cefuroxime groups (p=0.027). A significant difference was also observed in total oxidant status levels between the cefuroxime and balanced salt solution groups (p=0.023). In addition, there were significant differences in total antioxidant status levels in corneal tissue between the cefuroxime and sham groups (p<0.001) and between the cefuroxime and balanced salt solution groups (p<0.001). Furthermore, significant differences were also observed in oxidative stress index levels between the cefuroxime and balanced salt solution groups (p=0.001) and between the cefuroxime and sham groups (p=0.026). According to the immunohistochemical staining results, a significant association with caspase-3 activity existed between the cefuroxime and balanced salt solution groups (p=0.007), while no significant difference was found with caspase-8 activity (p=0.541). Caspase-3 activity exhibited a significant relationship between the sham and balanced salt solution groups (p=0.018), but no relationship was found with caspase-8 activity (p=0.623).
Immunohistochemical examination revealed that intracameral cefuroxime increased apoptosis when compared to the sham and balanced salt solution groups. Moreover, intracameral cefuroxime increased oxidative stress in the cornea and simultaneously induced apoptosis.</description><identifier>ISSN: 0004-2749</identifier><identifier>ISSN: 1678-2925</identifier><identifier>EISSN: 1678-2925</identifier><identifier>DOI: 10.5935/0004-2749.20190067</identifier><identifier>PMID: 31116319</identifier><language>eng</language><publisher>Brazil: Conselho Brasileiro de Oftalmologia</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Apoptosis - drug effects ; Aryldialkylphosphatase - analysis ; Carboxylic Ester Hydrolases - analysis ; Caspase 3 - analysis ; Caspase 8 - analysis ; Cefuroxime - pharmacology ; Cornea - drug effects ; Cornea - metabolism ; Cornea - pathology ; Endothelium, Corneal - drug effects ; Endothelium, Corneal - metabolism ; Endothelium, Corneal - pathology ; Immunohistochemistry ; Injections, Intraocular ; Male ; OPHTHALMOLOGY ; Oxidants - blood ; Oxidative Stress - drug effects ; Rats, Wistar ; Reproducibility of Results</subject><ispartof>Arquivos brasileiros de oftalmologia, 2019-07, Vol.82 (4), p.322-328</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31116319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozkan, Ufuk</creatorcontrib><creatorcontrib>Akal, Ali</creatorcontrib><creatorcontrib>Ozkan, Kudret</creatorcontrib><creatorcontrib>Yilmaz, Omer Faruk</creatorcontrib><creatorcontrib>Adıbelli, Fatih Mehmet</creatorcontrib><title>Investigating the effect of intracameral cefuroxime on oxidative stress and apoptosis in the rat cornea</title><title>Arquivos brasileiros de oftalmologia</title><addtitle>Arq Bras Oftalmol</addtitle><description>We examined the effect of intracameral administration of cefuroxime on oxidative stress and endothelial apoptosis in rat corneal tissue.
In total, 30 rats were divided into 3 groups of 10 rats each (intracameral administration of cefuroxime 0.1 mg/0.01 mL (cefuroxime group); intracameral administration of balanced salt solution 0.01 mL (control group); or absence of intracameral injection (sham group). Corneal endothelial apoptosis was assessed by immunohistochemical analysis using caspase-3 and caspase-8. Total oxidant status, total antioxidant status, oxidative stress index, and paraoxonase and arylesterase levels were examined in corneal endothelial tissue and serum.
Paraoxonase levels in the serum were significantly different between the sham and cefuroxime groups (p=0.027). A significant difference was also observed in total oxidant status levels between the cefuroxime and balanced salt solution groups (p=0.023). In addition, there were significant differences in total antioxidant status levels in corneal tissue between the cefuroxime and sham groups (p<0.001) and between the cefuroxime and balanced salt solution groups (p<0.001). Furthermore, significant differences were also observed in oxidative stress index levels between the cefuroxime and balanced salt solution groups (p=0.001) and between the cefuroxime and sham groups (p=0.026). According to the immunohistochemical staining results, a significant association with caspase-3 activity existed between the cefuroxime and balanced salt solution groups (p=0.007), while no significant difference was found with caspase-8 activity (p=0.541). Caspase-3 activity exhibited a significant relationship between the sham and balanced salt solution groups (p=0.018), but no relationship was found with caspase-8 activity (p=0.623).
Immunohistochemical examination revealed that intracameral cefuroxime increased apoptosis when compared to the sham and balanced salt solution groups. Moreover, intracameral cefuroxime increased oxidative stress in the cornea and simultaneously induced apoptosis.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Aryldialkylphosphatase - analysis</subject><subject>Carboxylic Ester Hydrolases - analysis</subject><subject>Caspase 3 - analysis</subject><subject>Caspase 8 - analysis</subject><subject>Cefuroxime - pharmacology</subject><subject>Cornea - drug effects</subject><subject>Cornea - metabolism</subject><subject>Cornea - pathology</subject><subject>Endothelium, Corneal - drug effects</subject><subject>Endothelium, Corneal - metabolism</subject><subject>Endothelium, Corneal - pathology</subject><subject>Immunohistochemistry</subject><subject>Injections, Intraocular</subject><subject>Male</subject><subject>OPHTHALMOLOGY</subject><subject>Oxidants - blood</subject><subject>Oxidative Stress - drug effects</subject><subject>Rats, Wistar</subject><subject>Reproducibility of Results</subject><issn>0004-2749</issn><issn>1678-2925</issn><issn>1678-2925</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kTFv2zAQhYmiRe2k_QMZAo5d5BxJURTHwmgbAwEyJJ0Jij66CiTRJSmj-fel49jT3fC-d7j3CLlhsJJayDsAqCuuar3iwDRAoz6QJWtUW3HN5UeyvAgW5CqlFwBeay0_k4VgjDWC6SXZbaYDptzvbO6nHc1_kKL36DINnvZTjtbZEaMdqEM_x_CvH5GGiZZlW5AD0pQjpkTttKV2H_Y5pD4V8s0q2kxdiBPaL-STt0PCr-_zmvz--eN5fV89PP7arL8_VE4IlSvV6FZKzWsQHWtko113fMK3dSc9KPSsAyZQOIQWhPBeKQkaHffci0YLcU1WJ9_kehyCeQlznMpB83T0MccwTmFBDSA4L8C3E7CP4e9cojBjnxwOg50wzMkUCYeWqVoVKT9JXQwpRfRmH_vRxlfDwBwbMZcb5txIgW7f_eduxO0FOVcg_gMZf4OK</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Ozkan, Ufuk</creator><creator>Akal, Ali</creator><creator>Ozkan, Kudret</creator><creator>Yilmaz, Omer Faruk</creator><creator>Adıbelli, Fatih Mehmet</creator><general>Conselho Brasileiro de Oftalmologia</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>20190701</creationdate><title>Investigating the effect of intracameral cefuroxime on oxidative stress and apoptosis in the rat cornea</title><author>Ozkan, Ufuk ; Akal, Ali ; Ozkan, Kudret ; Yilmaz, Omer Faruk ; Adıbelli, Fatih Mehmet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-76985592403b16569cb0004f84b5f07ef1b013e3ce08033ff77509ec2f2f36933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Aryldialkylphosphatase - analysis</topic><topic>Carboxylic Ester Hydrolases - analysis</topic><topic>Caspase 3 - analysis</topic><topic>Caspase 8 - analysis</topic><topic>Cefuroxime - pharmacology</topic><topic>Cornea - drug effects</topic><topic>Cornea - metabolism</topic><topic>Cornea - pathology</topic><topic>Endothelium, Corneal - drug effects</topic><topic>Endothelium, Corneal - metabolism</topic><topic>Endothelium, Corneal - pathology</topic><topic>Immunohistochemistry</topic><topic>Injections, Intraocular</topic><topic>Male</topic><topic>OPHTHALMOLOGY</topic><topic>Oxidants - blood</topic><topic>Oxidative Stress - drug effects</topic><topic>Rats, Wistar</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozkan, Ufuk</creatorcontrib><creatorcontrib>Akal, Ali</creatorcontrib><creatorcontrib>Ozkan, Kudret</creatorcontrib><creatorcontrib>Yilmaz, Omer Faruk</creatorcontrib><creatorcontrib>Adıbelli, Fatih Mehmet</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Arquivos brasileiros de oftalmologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozkan, Ufuk</au><au>Akal, Ali</au><au>Ozkan, Kudret</au><au>Yilmaz, Omer Faruk</au><au>Adıbelli, Fatih Mehmet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigating the effect of intracameral cefuroxime on oxidative stress and apoptosis in the rat cornea</atitle><jtitle>Arquivos brasileiros de oftalmologia</jtitle><addtitle>Arq Bras Oftalmol</addtitle><date>2019-07-01</date><risdate>2019</risdate><volume>82</volume><issue>4</issue><spage>322</spage><epage>328</epage><pages>322-328</pages><issn>0004-2749</issn><issn>1678-2925</issn><eissn>1678-2925</eissn><abstract>We examined the effect of intracameral administration of cefuroxime on oxidative stress and endothelial apoptosis in rat corneal tissue.
In total, 30 rats were divided into 3 groups of 10 rats each (intracameral administration of cefuroxime 0.1 mg/0.01 mL (cefuroxime group); intracameral administration of balanced salt solution 0.01 mL (control group); or absence of intracameral injection (sham group). Corneal endothelial apoptosis was assessed by immunohistochemical analysis using caspase-3 and caspase-8. Total oxidant status, total antioxidant status, oxidative stress index, and paraoxonase and arylesterase levels were examined in corneal endothelial tissue and serum.
Paraoxonase levels in the serum were significantly different between the sham and cefuroxime groups (p=0.027). A significant difference was also observed in total oxidant status levels between the cefuroxime and balanced salt solution groups (p=0.023). In addition, there were significant differences in total antioxidant status levels in corneal tissue between the cefuroxime and sham groups (p<0.001) and between the cefuroxime and balanced salt solution groups (p<0.001). Furthermore, significant differences were also observed in oxidative stress index levels between the cefuroxime and balanced salt solution groups (p=0.001) and between the cefuroxime and sham groups (p=0.026). According to the immunohistochemical staining results, a significant association with caspase-3 activity existed between the cefuroxime and balanced salt solution groups (p=0.007), while no significant difference was found with caspase-8 activity (p=0.541). Caspase-3 activity exhibited a significant relationship between the sham and balanced salt solution groups (p=0.018), but no relationship was found with caspase-8 activity (p=0.623).
Immunohistochemical examination revealed that intracameral cefuroxime increased apoptosis when compared to the sham and balanced salt solution groups. Moreover, intracameral cefuroxime increased oxidative stress in the cornea and simultaneously induced apoptosis.</abstract><cop>Brazil</cop><pub>Conselho Brasileiro de Oftalmologia</pub><pmid>31116319</pmid><doi>10.5935/0004-2749.20190067</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Anti-Bacterial Agents - pharmacology Apoptosis - drug effects Aryldialkylphosphatase - analysis Carboxylic Ester Hydrolases - analysis Caspase 3 - analysis Caspase 8 - analysis Cefuroxime - pharmacology Cornea - drug effects Cornea - metabolism Cornea - pathology Endothelium, Corneal - drug effects Endothelium, Corneal - metabolism Endothelium, Corneal - pathology Immunohistochemistry Injections, Intraocular Male OPHTHALMOLOGY Oxidants - blood Oxidative Stress - drug effects Rats, Wistar Reproducibility of Results |
| title | Investigating the effect of intracameral cefuroxime on oxidative stress and apoptosis in the rat cornea |
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