Polyacrylamide Gel Electrophoresis: Hormonal and Species Specificity Of Antibody Binding of Bovine I131 Thyrotropin.

Polyacrylamide Gel Electrophoresis: Hormonal and Species Specificity Of Antibody Binding of Bovine I131 Thyrotropin.

Summary In polyacrylamide gel electro-phoresis of bovine I131-TSH the radioactivity and biological activity migrate similarly but with slight dissociation. Increasing quantities of antiserum shift increasing quantities of radioactivity to slower moving regions. Bovine TSH preparations interfere with...

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Veröffentlicht in:Experimental biology and medicine (Maywood, N.J.) N.J.), 1966-07, Vol.122 (3), p.795-799
Hauptverfasser: Heideman, M. Lawrence, McGuire, William L., Levy, Richard P., Shipley, Reginald A.
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container_issue 3
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container_title Experimental biology and medicine (Maywood, N.J.)
container_volume 122
creator Heideman, M. Lawrence
McGuire, William L.
Levy, Richard P.
Shipley, Reginald A.
description Summary In polyacrylamide gel electro-phoresis of bovine I131-TSH the radioactivity and biological activity migrate similarly but with slight dissociation. Increasing quantities of antiserum shift increasing quantities of radioactivity to slower moving regions. Bovine TSH preparations interfere with I131-TSH binding to antibody in proportion to biological activity. Human TSH is much less active. We thank Messrs. Frank Zodnik and Donald Eros for technical assistance; Drs. Robert W. Bates, Mary E. Carsten and John G. Pierce, and Peter G. Cond-liffe for supplying purified bovine TSH preparations.
doi_str_mv 10.3181/00379727-122-31254
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title Polyacrylamide Gel Electrophoresis: Hormonal and Species Specificity Of Antibody Binding of Bovine I131 Thyrotropin.
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