Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells and in a critical-size femoral defect model in rats
The borosilicate 0106-B1-bioactive glass (BG) composition (in wt%: 37.5 SiO 2 , 22.6 CaO, 5.9 Na 2 O, 4.0P 2 O 5 , 12.0 K 2 O, 5.5 MgO, 12.5 B 2 O 3 ) has shown favorable processing characteristics and bone regeneration ability. This study investigated the addition of zinc (Zn) to 0106-B1-BG as an a...
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| Veröffentlicht in: | Biomaterials science 2024-09, Vol.12 (18), p.477-4789 |
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| creator | Saur, M Kunisch, E Fiehn, L. A Arango-Ospina, M Merle, C Hagmann, S Moghaddam, A Stiller, A Hupa, L Renkawitz, T Ka ková, H Galusková, D Boccaccini, A. R Westhauser, F |
| description | The borosilicate 0106-B1-bioactive glass (BG) composition (in wt%: 37.5 SiO
2
, 22.6 CaO, 5.9 Na
2
O, 4.0P
2
O
5
, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
) has shown favorable processing characteristics and bone regeneration ability. This study investigated the addition of zinc (Zn) to 0106-B1-BG as an approach to improve this BG's biological properties. Different proportions of ZnO were substituted for CaO in 0106-B1-BG, resulting in three new BG-compositions: 1-Zn-BG, 2-Zn-BG, 3-Zn-BG (in wt%: 37.5 SiO
2
, 21.6/20.1/17.6 CaO, 4.0 P
2
O
5
, 5.9 Na
2
O, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
and 1.0/2.5/5.0 ZnO). Effects of the BG compositions on cytocompatibility, osteogenic differentiation, extracellular matrix deposition, and angiogenic response of human bone marrow-derived mesenchymal stromal cells (BMSCs) were evaluated
in vitro
. Angiogenic effects were assessed using a tube formation assay containing human umbilical vein endothelial cells. The
in vivo
osteogenic and angiogenic potentials of 3-Zn-BG were investigated in comparison to the Zn-free 0106-B1-BG in a rodent critical-size femoral defect model. The osteogenic differentiation of BMSCs improved in the presence of Zn. 3-Zn-BG showed enhanced angiogenic potential, as confirmed by the tube formation assay. While Zn-doped BGs showed clearly superior biological properties
in vitro
, 3-Zn-BG and 0106-B1-BG equally promoted the formation of new bone
in vivo
; however, 3-Zn-BG reduced osteoclastic cells and vascular structures
in vivo
. The acquired data suggests that the differences regarding the
in vivo
and
in vitro
results may be due to modulation of inflammatory responses by Zn, as described in the literature. The inflammatory effect should be investigated further to promote clinical applications of Zn-doped BGs.
Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells
in vitro
and in a critical-size femoral defect model in rats
in vivo
. |
| doi_str_mv | 10.1039/d4bm00529e |
| format | Article |
| fullrecord | <record><control><sourceid>rsc</sourceid><recordid>TN_cdi_rsc_primary_d4bm00529e</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>d4bm00529e</sourcerecordid><originalsourceid>FETCH-rsc_primary_d4bm00529e3</originalsourceid><addsrcrecordid>eNqFj8FKBDEMQIsouLh78S7kB0Y7O6PjXBXFD_C-dNp0jbSNNB3F_R4_1A6IHs0lgbzkJUqdt_qy1d145fopan29HfFIrba6H5r-th-Pf-tOn6qNyKuuMQyjvmlX6uuOOPCerAmA3qMtAuzBwIGSbWSepFCZCzqYOLNQqGRBmIiNLfSOsA9G6kiClzmaVKmEEE3O_AEOcyUcSMkc636LIQiY5IBSNdhMZfE2QgcEj5FzhRwuR0Bkh2HhsimyVifeBMHNTz5TF48Pz_dPTRa7e8tUfZ-7v--7__rfn_BhDg</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells and in a critical-size femoral defect model in rats</title><source>Royal Society Of Chemistry Journals 2008-</source><creator>Saur, M ; Kunisch, E ; Fiehn, L. A ; Arango-Ospina, M ; Merle, C ; Hagmann, S ; Moghaddam, A ; Stiller, A ; Hupa, L ; Renkawitz, T ; Ka ková, H ; Galusková, D ; Boccaccini, A. R ; Westhauser, F</creator><creatorcontrib>Saur, M ; Kunisch, E ; Fiehn, L. A ; Arango-Ospina, M ; Merle, C ; Hagmann, S ; Moghaddam, A ; Stiller, A ; Hupa, L ; Renkawitz, T ; Ka ková, H ; Galusková, D ; Boccaccini, A. R ; Westhauser, F</creatorcontrib><description>The borosilicate 0106-B1-bioactive glass (BG) composition (in wt%: 37.5 SiO
2
, 22.6 CaO, 5.9 Na
2
O, 4.0P
2
O
5
, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
) has shown favorable processing characteristics and bone regeneration ability. This study investigated the addition of zinc (Zn) to 0106-B1-BG as an approach to improve this BG's biological properties. Different proportions of ZnO were substituted for CaO in 0106-B1-BG, resulting in three new BG-compositions: 1-Zn-BG, 2-Zn-BG, 3-Zn-BG (in wt%: 37.5 SiO
2
, 21.6/20.1/17.6 CaO, 4.0 P
2
O
5
, 5.9 Na
2
O, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
and 1.0/2.5/5.0 ZnO). Effects of the BG compositions on cytocompatibility, osteogenic differentiation, extracellular matrix deposition, and angiogenic response of human bone marrow-derived mesenchymal stromal cells (BMSCs) were evaluated
in vitro
. Angiogenic effects were assessed using a tube formation assay containing human umbilical vein endothelial cells. The
in vivo
osteogenic and angiogenic potentials of 3-Zn-BG were investigated in comparison to the Zn-free 0106-B1-BG in a rodent critical-size femoral defect model. The osteogenic differentiation of BMSCs improved in the presence of Zn. 3-Zn-BG showed enhanced angiogenic potential, as confirmed by the tube formation assay. While Zn-doped BGs showed clearly superior biological properties
in vitro
, 3-Zn-BG and 0106-B1-BG equally promoted the formation of new bone
in vivo
; however, 3-Zn-BG reduced osteoclastic cells and vascular structures
in vivo
. The acquired data suggests that the differences regarding the
in vivo
and
in vitro
results may be due to modulation of inflammatory responses by Zn, as described in the literature. The inflammatory effect should be investigated further to promote clinical applications of Zn-doped BGs.
Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells
in vitro
and in a critical-size femoral defect model in rats
in vivo
.</description><identifier>ISSN: 2047-4830</identifier><identifier>EISSN: 2047-4849</identifier><identifier>DOI: 10.1039/d4bm00529e</identifier><ispartof>Biomaterials science, 2024-09, Vol.12 (18), p.477-4789</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Saur, M</creatorcontrib><creatorcontrib>Kunisch, E</creatorcontrib><creatorcontrib>Fiehn, L. A</creatorcontrib><creatorcontrib>Arango-Ospina, M</creatorcontrib><creatorcontrib>Merle, C</creatorcontrib><creatorcontrib>Hagmann, S</creatorcontrib><creatorcontrib>Moghaddam, A</creatorcontrib><creatorcontrib>Stiller, A</creatorcontrib><creatorcontrib>Hupa, L</creatorcontrib><creatorcontrib>Renkawitz, T</creatorcontrib><creatorcontrib>Ka ková, H</creatorcontrib><creatorcontrib>Galusková, D</creatorcontrib><creatorcontrib>Boccaccini, A. R</creatorcontrib><creatorcontrib>Westhauser, F</creatorcontrib><title>Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells and in a critical-size femoral defect model in rats</title><title>Biomaterials science</title><description>The borosilicate 0106-B1-bioactive glass (BG) composition (in wt%: 37.5 SiO
2
, 22.6 CaO, 5.9 Na
2
O, 4.0P
2
O
5
, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
) has shown favorable processing characteristics and bone regeneration ability. This study investigated the addition of zinc (Zn) to 0106-B1-BG as an approach to improve this BG's biological properties. Different proportions of ZnO were substituted for CaO in 0106-B1-BG, resulting in three new BG-compositions: 1-Zn-BG, 2-Zn-BG, 3-Zn-BG (in wt%: 37.5 SiO
2
, 21.6/20.1/17.6 CaO, 4.0 P
2
O
5
, 5.9 Na
2
O, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
and 1.0/2.5/5.0 ZnO). Effects of the BG compositions on cytocompatibility, osteogenic differentiation, extracellular matrix deposition, and angiogenic response of human bone marrow-derived mesenchymal stromal cells (BMSCs) were evaluated
in vitro
. Angiogenic effects were assessed using a tube formation assay containing human umbilical vein endothelial cells. The
in vivo
osteogenic and angiogenic potentials of 3-Zn-BG were investigated in comparison to the Zn-free 0106-B1-BG in a rodent critical-size femoral defect model. The osteogenic differentiation of BMSCs improved in the presence of Zn. 3-Zn-BG showed enhanced angiogenic potential, as confirmed by the tube formation assay. While Zn-doped BGs showed clearly superior biological properties
in vitro
, 3-Zn-BG and 0106-B1-BG equally promoted the formation of new bone
in vivo
; however, 3-Zn-BG reduced osteoclastic cells and vascular structures
in vivo
. The acquired data suggests that the differences regarding the
in vivo
and
in vitro
results may be due to modulation of inflammatory responses by Zn, as described in the literature. The inflammatory effect should be investigated further to promote clinical applications of Zn-doped BGs.
Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells
in vitro
and in a critical-size femoral defect model in rats
in vivo
.</description><issn>2047-4830</issn><issn>2047-4849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFj8FKBDEMQIsouLh78S7kB0Y7O6PjXBXFD_C-dNp0jbSNNB3F_R4_1A6IHs0lgbzkJUqdt_qy1d145fopan29HfFIrba6H5r-th-Pf-tOn6qNyKuuMQyjvmlX6uuOOPCerAmA3qMtAuzBwIGSbWSepFCZCzqYOLNQqGRBmIiNLfSOsA9G6kiClzmaVKmEEE3O_AEOcyUcSMkc636LIQiY5IBSNdhMZfE2QgcEj5FzhRwuR0Bkh2HhsimyVifeBMHNTz5TF48Pz_dPTRa7e8tUfZ-7v--7__rfn_BhDg</recordid><startdate>20240910</startdate><enddate>20240910</enddate><creator>Saur, M</creator><creator>Kunisch, E</creator><creator>Fiehn, L. A</creator><creator>Arango-Ospina, M</creator><creator>Merle, C</creator><creator>Hagmann, S</creator><creator>Moghaddam, A</creator><creator>Stiller, A</creator><creator>Hupa, L</creator><creator>Renkawitz, T</creator><creator>Ka ková, H</creator><creator>Galusková, D</creator><creator>Boccaccini, A. R</creator><creator>Westhauser, F</creator><scope/></search><sort><creationdate>20240910</creationdate><title>Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells and in a critical-size femoral defect model in rats</title><author>Saur, M ; Kunisch, E ; Fiehn, L. A ; Arango-Ospina, M ; Merle, C ; Hagmann, S ; Moghaddam, A ; Stiller, A ; Hupa, L ; Renkawitz, T ; Ka ková, H ; Galusková, D ; Boccaccini, A. R ; Westhauser, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-rsc_primary_d4bm00529e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saur, M</creatorcontrib><creatorcontrib>Kunisch, E</creatorcontrib><creatorcontrib>Fiehn, L. A</creatorcontrib><creatorcontrib>Arango-Ospina, M</creatorcontrib><creatorcontrib>Merle, C</creatorcontrib><creatorcontrib>Hagmann, S</creatorcontrib><creatorcontrib>Moghaddam, A</creatorcontrib><creatorcontrib>Stiller, A</creatorcontrib><creatorcontrib>Hupa, L</creatorcontrib><creatorcontrib>Renkawitz, T</creatorcontrib><creatorcontrib>Ka ková, H</creatorcontrib><creatorcontrib>Galusková, D</creatorcontrib><creatorcontrib>Boccaccini, A. R</creatorcontrib><creatorcontrib>Westhauser, F</creatorcontrib><jtitle>Biomaterials science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saur, M</au><au>Kunisch, E</au><au>Fiehn, L. A</au><au>Arango-Ospina, M</au><au>Merle, C</au><au>Hagmann, S</au><au>Moghaddam, A</au><au>Stiller, A</au><au>Hupa, L</au><au>Renkawitz, T</au><au>Ka ková, H</au><au>Galusková, D</au><au>Boccaccini, A. R</au><au>Westhauser, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells and in a critical-size femoral defect model in rats</atitle><jtitle>Biomaterials science</jtitle><date>2024-09-10</date><risdate>2024</risdate><volume>12</volume><issue>18</issue><spage>477</spage><epage>4789</epage><pages>477-4789</pages><issn>2047-4830</issn><eissn>2047-4849</eissn><abstract>The borosilicate 0106-B1-bioactive glass (BG) composition (in wt%: 37.5 SiO
2
, 22.6 CaO, 5.9 Na
2
O, 4.0P
2
O
5
, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
) has shown favorable processing characteristics and bone regeneration ability. This study investigated the addition of zinc (Zn) to 0106-B1-BG as an approach to improve this BG's biological properties. Different proportions of ZnO were substituted for CaO in 0106-B1-BG, resulting in three new BG-compositions: 1-Zn-BG, 2-Zn-BG, 3-Zn-BG (in wt%: 37.5 SiO
2
, 21.6/20.1/17.6 CaO, 4.0 P
2
O
5
, 5.9 Na
2
O, 12.0 K
2
O, 5.5 MgO, 12.5 B
2
O
3
and 1.0/2.5/5.0 ZnO). Effects of the BG compositions on cytocompatibility, osteogenic differentiation, extracellular matrix deposition, and angiogenic response of human bone marrow-derived mesenchymal stromal cells (BMSCs) were evaluated
in vitro
. Angiogenic effects were assessed using a tube formation assay containing human umbilical vein endothelial cells. The
in vivo
osteogenic and angiogenic potentials of 3-Zn-BG were investigated in comparison to the Zn-free 0106-B1-BG in a rodent critical-size femoral defect model. The osteogenic differentiation of BMSCs improved in the presence of Zn. 3-Zn-BG showed enhanced angiogenic potential, as confirmed by the tube formation assay. While Zn-doped BGs showed clearly superior biological properties
in vitro
, 3-Zn-BG and 0106-B1-BG equally promoted the formation of new bone
in vivo
; however, 3-Zn-BG reduced osteoclastic cells and vascular structures
in vivo
. The acquired data suggests that the differences regarding the
in vivo
and
in vitro
results may be due to modulation of inflammatory responses by Zn, as described in the literature. The inflammatory effect should be investigated further to promote clinical applications of Zn-doped BGs.
Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells
in vitro
and in a critical-size femoral defect model in rats
in vivo
.</abstract><doi>10.1039/d4bm00529e</doi><tpages>2</tpages></addata></record> |
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| source | Royal Society Of Chemistry Journals 2008- |
| title | Biological effects of a zinc-substituted borosilicate bioactive glass on human bone marrow derived stromal cells and in a critical-size femoral defect model in rats |
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