Preparation of hydroxyapatite coated porous carbon nanofibres for DEX loading and enhancing differentiation of BMSCs
The proliferation and differentiation of bone mesenchymal stem cells (BMSCs) in vitro are the key properties of bone tissue engineering for biomaterials. In this study, hydroxyapatite (HA) coated porous carbon nanofibres (PCNFs) were prepared to load dexamethasone (DEX) and further improve the diffe...
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| Veröffentlicht in: | RSC advances 2023-10, Vol.13 (44), p.3898-394 |
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| creator | Lan, Liujia Zhang, Qian Zhang, Huiyun Yang, Xiaochuan Li, Suying Li, Guang Luo, Yi Nie, Du Zhang, Guangyu Dai, Jiamu |
| description | The proliferation and differentiation of bone mesenchymal stem cells (BMSCs)
in vitro
are the key properties of bone tissue engineering for biomaterials. In this study, hydroxyapatite (HA) coated porous carbon nanofibres (PCNFs) were prepared to load dexamethasone (DEX) and further improve the differentiation ability of the BMSCs. Various characterisations were applied to reveal the DEX loading efficacy and biocompatibility, especially the differentiation strength. The results showed that HA could be successfully coated on the PCNFs by pretreating the surface using PEG conjugation. With an increase of HA, the particle diameter increased and the DEX loading decreased.
In vitro
experiments proved higher cell viability, alkaline phosphatase (ALP) activity, calcium nodule secretion ability and the RUNX2 protein expression, indicating that the as-prepared was of great biocompatibility and optimised osteoconductivity, which was attributed to the componential imitation to natural bone and the accelerated BMSCs differentiation. Consequently, the novel DEX loaded and HA coated PCNFs can provide potential applications in bone tissue regeneration.
This study aims to prepare DEX loaded and HA coated carbon porous carbon nanofibers. The released DEX and osteoconductivity of HA are proved to accelerate osteo differentiation of BMSCs. |
| doi_str_mv | 10.1039/d3ra02107f |
| format | Article |
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in vitro
are the key properties of bone tissue engineering for biomaterials. In this study, hydroxyapatite (HA) coated porous carbon nanofibres (PCNFs) were prepared to load dexamethasone (DEX) and further improve the differentiation ability of the BMSCs. Various characterisations were applied to reveal the DEX loading efficacy and biocompatibility, especially the differentiation strength. The results showed that HA could be successfully coated on the PCNFs by pretreating the surface using PEG conjugation. With an increase of HA, the particle diameter increased and the DEX loading decreased.
In vitro
experiments proved higher cell viability, alkaline phosphatase (ALP) activity, calcium nodule secretion ability and the RUNX2 protein expression, indicating that the as-prepared was of great biocompatibility and optimised osteoconductivity, which was attributed to the componential imitation to natural bone and the accelerated BMSCs differentiation. Consequently, the novel DEX loaded and HA coated PCNFs can provide potential applications in bone tissue regeneration.
This study aims to prepare DEX loaded and HA coated carbon porous carbon nanofibers. The released DEX and osteoconductivity of HA are proved to accelerate osteo differentiation of BMSCs.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d3ra02107f</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Alkaline phosphatase ; Biocompatibility ; Biomedical materials ; Bones ; Carbon fibers ; Chemistry ; Conjugation ; Dexamethasone ; Differentiation ; Hydroxyapatite ; Nanofibers ; Particle size ; Regeneration (physiology) ; Stem cells ; Tissue engineering</subject><ispartof>RSC advances, 2023-10, Vol.13 (44), p.3898-394</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><rights>This journal is © The Royal Society of Chemistry 2023 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-ef1d43bd85e86a559506996f7742cc48ce6c48126fb9567f9f4b229163cbfde43</citedby><cites>FETCH-LOGICAL-c406t-ef1d43bd85e86a559506996f7742cc48ce6c48126fb9567f9f4b229163cbfde43</cites><orcidid>0000-0002-7456-826X ; 0000-0002-1364-3350 ; 0000-0002-0627-4999</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588370/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588370/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,53796,53798</link.rule.ids></links><search><creatorcontrib>Lan, Liujia</creatorcontrib><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Zhang, Huiyun</creatorcontrib><creatorcontrib>Yang, Xiaochuan</creatorcontrib><creatorcontrib>Li, Suying</creatorcontrib><creatorcontrib>Li, Guang</creatorcontrib><creatorcontrib>Luo, Yi</creatorcontrib><creatorcontrib>Nie, Du</creatorcontrib><creatorcontrib>Zhang, Guangyu</creatorcontrib><creatorcontrib>Dai, Jiamu</creatorcontrib><title>Preparation of hydroxyapatite coated porous carbon nanofibres for DEX loading and enhancing differentiation of BMSCs</title><title>RSC advances</title><description>The proliferation and differentiation of bone mesenchymal stem cells (BMSCs)
in vitro
are the key properties of bone tissue engineering for biomaterials. In this study, hydroxyapatite (HA) coated porous carbon nanofibres (PCNFs) were prepared to load dexamethasone (DEX) and further improve the differentiation ability of the BMSCs. Various characterisations were applied to reveal the DEX loading efficacy and biocompatibility, especially the differentiation strength. The results showed that HA could be successfully coated on the PCNFs by pretreating the surface using PEG conjugation. With an increase of HA, the particle diameter increased and the DEX loading decreased.
In vitro
experiments proved higher cell viability, alkaline phosphatase (ALP) activity, calcium nodule secretion ability and the RUNX2 protein expression, indicating that the as-prepared was of great biocompatibility and optimised osteoconductivity, which was attributed to the componential imitation to natural bone and the accelerated BMSCs differentiation. Consequently, the novel DEX loaded and HA coated PCNFs can provide potential applications in bone tissue regeneration.
This study aims to prepare DEX loaded and HA coated carbon porous carbon nanofibers. The released DEX and osteoconductivity of HA are proved to accelerate osteo differentiation of BMSCs.</description><subject>Alkaline phosphatase</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Bones</subject><subject>Carbon fibers</subject><subject>Chemistry</subject><subject>Conjugation</subject><subject>Dexamethasone</subject><subject>Differentiation</subject><subject>Hydroxyapatite</subject><subject>Nanofibers</subject><subject>Particle size</subject><subject>Regeneration (physiology)</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpdkc9LHDEcxQdpQbFeehcCXkphbZLJZDInsau2BUtLq-AtZJJv3MhsMv1mtrj_vdmubFtzyM8Pj_fyquoto6eM1t0HV6OhnNHW71UHnAo541R2r_7Z71dHOT_QMmTDuGQH1fQdYTRoppAiSZ4s1g7T49qM5WYCYpOZwJExYVplYg32BYsmJh96hEx8QnJxeUeGZFyI98RERyAuTLSbkwveA0Kcwk7_49ef8_ymeu3NkOHoeT2sbq8ub-afZ9ffPn2Zn1_PrKBymoFnTtS9Uw0oaZqma0qETvq2FdxaoSzIMpccvu8a2frOi57zjsna9t6BqA-rs63uuOqX4GxxgmbQI4alwbVOJuj_X2JY6Pv0WzPaKFW3tCi8e1bA9GsFedLLkC0Mg4lQfkRzpajigoumoCcv0Ie0wljybSghhWRqY-n9lrKYckbwOzeM6k2L-qL-cf6nxasCH29hzHbH_W25fgJ8c5sN</recordid><startdate>20231020</startdate><enddate>20231020</enddate><creator>Lan, Liujia</creator><creator>Zhang, Qian</creator><creator>Zhang, Huiyun</creator><creator>Yang, Xiaochuan</creator><creator>Li, Suying</creator><creator>Li, Guang</creator><creator>Luo, Yi</creator><creator>Nie, Du</creator><creator>Zhang, Guangyu</creator><creator>Dai, Jiamu</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7456-826X</orcidid><orcidid>https://orcid.org/0000-0002-1364-3350</orcidid><orcidid>https://orcid.org/0000-0002-0627-4999</orcidid></search><sort><creationdate>20231020</creationdate><title>Preparation of hydroxyapatite coated porous carbon nanofibres for DEX loading and enhancing differentiation of BMSCs</title><author>Lan, Liujia ; Zhang, Qian ; Zhang, Huiyun ; Yang, Xiaochuan ; Li, Suying ; Li, Guang ; Luo, Yi ; Nie, Du ; Zhang, Guangyu ; Dai, Jiamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-ef1d43bd85e86a559506996f7742cc48ce6c48126fb9567f9f4b229163cbfde43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alkaline phosphatase</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Bones</topic><topic>Carbon fibers</topic><topic>Chemistry</topic><topic>Conjugation</topic><topic>Dexamethasone</topic><topic>Differentiation</topic><topic>Hydroxyapatite</topic><topic>Nanofibers</topic><topic>Particle size</topic><topic>Regeneration (physiology)</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lan, Liujia</creatorcontrib><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Zhang, Huiyun</creatorcontrib><creatorcontrib>Yang, Xiaochuan</creatorcontrib><creatorcontrib>Li, Suying</creatorcontrib><creatorcontrib>Li, Guang</creatorcontrib><creatorcontrib>Luo, Yi</creatorcontrib><creatorcontrib>Nie, Du</creatorcontrib><creatorcontrib>Zhang, Guangyu</creatorcontrib><creatorcontrib>Dai, Jiamu</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lan, Liujia</au><au>Zhang, Qian</au><au>Zhang, Huiyun</au><au>Yang, Xiaochuan</au><au>Li, Suying</au><au>Li, Guang</au><au>Luo, Yi</au><au>Nie, Du</au><au>Zhang, Guangyu</au><au>Dai, Jiamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of hydroxyapatite coated porous carbon nanofibres for DEX loading and enhancing differentiation of BMSCs</atitle><jtitle>RSC advances</jtitle><date>2023-10-20</date><risdate>2023</risdate><volume>13</volume><issue>44</issue><spage>3898</spage><epage>394</epage><pages>3898-394</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>The proliferation and differentiation of bone mesenchymal stem cells (BMSCs)
in vitro
are the key properties of bone tissue engineering for biomaterials. In this study, hydroxyapatite (HA) coated porous carbon nanofibres (PCNFs) were prepared to load dexamethasone (DEX) and further improve the differentiation ability of the BMSCs. Various characterisations were applied to reveal the DEX loading efficacy and biocompatibility, especially the differentiation strength. The results showed that HA could be successfully coated on the PCNFs by pretreating the surface using PEG conjugation. With an increase of HA, the particle diameter increased and the DEX loading decreased.
In vitro
experiments proved higher cell viability, alkaline phosphatase (ALP) activity, calcium nodule secretion ability and the RUNX2 protein expression, indicating that the as-prepared was of great biocompatibility and optimised osteoconductivity, which was attributed to the componential imitation to natural bone and the accelerated BMSCs differentiation. Consequently, the novel DEX loaded and HA coated PCNFs can provide potential applications in bone tissue regeneration.
This study aims to prepare DEX loaded and HA coated carbon porous carbon nanofibers. The released DEX and osteoconductivity of HA are proved to accelerate osteo differentiation of BMSCs.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d3ra02107f</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7456-826X</orcidid><orcidid>https://orcid.org/0000-0002-1364-3350</orcidid><orcidid>https://orcid.org/0000-0002-0627-4999</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Alkaline phosphatase Biocompatibility Biomedical materials Bones Carbon fibers Chemistry Conjugation Dexamethasone Differentiation Hydroxyapatite Nanofibers Particle size Regeneration (physiology) Stem cells Tissue engineering |
| title | Preparation of hydroxyapatite coated porous carbon nanofibres for DEX loading and enhancing differentiation of BMSCs |
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