Monitoring of phosphatase and kinase activity using P NMR spectroscopy

Monitoring of phosphatase and kinase activity using P NMR spectroscopy

Traditionally, enzymatic assays require the addition of labels or exogenous supplementation sources. Phosphorus-31 nuclear magnetic resonance ( 31 P NMR) spectroscopy has proven to be a powerful and reliable tool, but it is scarcely employed to evaluate enzymatic activity. Herein, we provide a versa...

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Veröffentlicht in:New journal of chemistry 2023-09, Vol.47 (35), p.16631-16635
Hauptverfasser: Guo, Xiaofan, Han, Bowen, Qiu, Wenhan, Deng, Peiran, Fu, Songsen, Ying, Jianxi, Zhao, Yufen
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container_end_page 16635
container_issue 35
container_start_page 16631
container_title New journal of chemistry
container_volume 47
creator Guo, Xiaofan
Han, Bowen
Qiu, Wenhan
Deng, Peiran
Fu, Songsen
Ying, Jianxi
Zhao, Yufen
description Traditionally, enzymatic assays require the addition of labels or exogenous supplementation sources. Phosphorus-31 nuclear magnetic resonance ( 31 P NMR) spectroscopy has proven to be a powerful and reliable tool, but it is scarcely employed to evaluate enzymatic activity. Herein, we provide a versatile method to directly characterize phosphatase and kinase activities by exploiting the apparently differential 31 P NMR shifts between substrates and products, without requiring tags or additional reagents. The utility of this method for inhibitor identification is also presented. We provide a new method for the direct identification of enzyme activity based on signal changes of phosphorus-containing small molecules in 31 P NMR spectroscopy.
doi_str_mv 10.1039/d3nj00642e
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title Monitoring of phosphatase and kinase activity using P NMR spectroscopy
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