Nano-optical method for transforming a single yeast cell using exogenous genes

We report a highly efficient nano-optical method for transforming a single yeast cell using exogenous genes. It used laser tweezers or micromanipulators to immobilize the cell immersed in a DNA solution and created a transient nano-sized hole on its cell wall concurrently with laser scissors to deli...

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Veröffentlicht in:	RSC advances 2022-11, Vol.12 (49), p.31846-31854
Hauptverfasser:	Huang, Yao-Xiong, Yang, Ji-Wang, Wang, Zhuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemistry Deoxyribonucleic acid DNA Fluorescence Gene sequencing Genes Micromanipulation Nucleic acids Transformations Yeast
Online-Zugang:	Volltext
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