Hyperpolarization study on remdesivir with its biological reaction monitoring signal amplification by reversible exchange

Our experiments indicate hyperpolarized proton signals in the entire structure of remdesivir are obtained due to a long-distance polarization transfer by para -hydrogen. SABRE-based biological real-time reaction monitoring, by using a protein enzyme under mild conditions is carried out. It represents the first successful para -hydrogen based hyperpolarization application in biological reaction monitoring. Hyperpolarized proton signals in the entire structure of remdesivir are obtained due to a long-distance polarization transfer by para -hydrogen. Biological real-time reaction monitoring, by using a protein enzyme under mild conditions is carried out.