A signal on-off strategy based on the digestion of DNA cubes assisted by the CRISPR-Cas12a system for ultrasensitive HBV detection in solid-state nanopores

Solid-state nanopores have been proven as a powerful platform for label-free single-molecule analysis. However, due to its relatively low resolution and selectivity, developing biosensors with good translocation signals faces two significant challenges: (1) small-sized chemical or biological targets...

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Veröffentlicht in:	Analyst (London) 2022-12, Vol.147 (24), p.5623-5632
Hauptverfasser:	Wang, Jiahai, Luo, Le, Li, Yunhui, Chen, Lanfang, Gui, Cenlin, Zhu, Jianji, Li, Huizhen, Wang, Wenlong, Chen, Daqi
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological properties Biosensors Buffers CRISPR Cubes Hepatitis B Noise levels Optimization Polymerase chain reaction Proteins Selectivity Signal to noise ratio Solid state Transducers
Online-Zugang:	Volltext
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