Methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy
Rhodamine fluorophores are setting benchmarks in fluorescence microscopy. Herein, we report the deuterium (d12) congeners of tetramethyl(silicon)rhodamine, obtained by isotopic labelling of the four methyl groups, show improved photophysical parameters ( i.e. brightness, lifetimes) and reduced chemi...
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| Veröffentlicht in: | Chemical science (Cambridge) 2022-07, Vol.13 (29), p.865-8617 |
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| creator | Roßmann, Kilian Akkaya, Kerem C Poc, Pascal Charbonnier, Corentin Eichhorst, Jenny Gonschior, Hannes Valavalkar, Abha Wendler, Nicolas Cordes, Thorben Dietzek-Ivanši, Benjamin Jones, Ben Lehmann, Martin Broichhagen, Johannes |
| description | Rhodamine fluorophores are setting benchmarks in fluorescence microscopy. Herein, we report the deuterium (d12) congeners of tetramethyl(silicon)rhodamine, obtained by isotopic labelling of the four methyl groups, show improved photophysical parameters (
i.e.
brightness, lifetimes) and reduced chemical bleaching. We explore this finding for SNAP- and Halo-tag labelling in live cells, and highlight enhanced properties in several applications, such as fluorescence activated cell sorting, fluorescence lifetime microscopy, stimulated emission depletion nanoscopy and single-molecule Förster-resonance energy transfer. We finally extend this idea to other dye families and envision deuteration as a generalizable concept to improve existing and to develop new chemical biology probes.
Deuteration enhances photophysical and chemical properties of fluorescent rhodamine dyes for higher brightness in sensitive microscopy. |
| doi_str_mv | 10.1039/d1sc06466e |
| format | Article |
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i.e.
brightness, lifetimes) and reduced chemical bleaching. We explore this finding for SNAP- and Halo-tag labelling in live cells, and highlight enhanced properties in several applications, such as fluorescence activated cell sorting, fluorescence lifetime microscopy, stimulated emission depletion nanoscopy and single-molecule Förster-resonance energy transfer. We finally extend this idea to other dye families and envision deuteration as a generalizable concept to improve existing and to develop new chemical biology probes.
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i.e.
brightness, lifetimes) and reduced chemical bleaching. We explore this finding for SNAP- and Halo-tag labelling in live cells, and highlight enhanced properties in several applications, such as fluorescence activated cell sorting, fluorescence lifetime microscopy, stimulated emission depletion nanoscopy and single-molecule Förster-resonance energy transfer. We finally extend this idea to other dye families and envision deuteration as a generalizable concept to improve existing and to develop new chemical biology probes.
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i.e.
brightness, lifetimes) and reduced chemical bleaching. We explore this finding for SNAP- and Halo-tag labelling in live cells, and highlight enhanced properties in several applications, such as fluorescence activated cell sorting, fluorescence lifetime microscopy, stimulated emission depletion nanoscopy and single-molecule Förster-resonance energy transfer. We finally extend this idea to other dye families and envision deuteration as a generalizable concept to improve existing and to develop new chemical biology probes.
Deuteration enhances photophysical and chemical properties of fluorescent rhodamine dyes for higher brightness in sensitive microscopy.</abstract><doi>10.1039/d1sc06466e</doi><tpages>13</tpages></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| title | Methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy |
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