Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy
The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection. This need has generated a high number of new testing methods aimed at replacing RT-PCR, which is the golden standard for testing. Most of the testing techniques are based on biochemistry methods...
Gespeichert in:
| Veröffentlicht in: | RSC advances 2021-07, Vol.11 (41), p.25788-25794 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 25794 |
|---|---|
| container_issue | 41 |
| container_start_page | 25788 |
| container_title | RSC advances |
| container_volume | 11 |
| creator | Sanchez, John Eder Jaramillo, Sierra A Settles, Erik Velazquez Salazar, J. Jesus Lehr, Alexander Gonzalez, Javier Rodríguez Aranda, Carmen Navarro-Contreras, Hugo R Raniere, Marlee O Harvey, Maxx Wagner, David M Koppisch, Andrew Kellar, Robert Keim, Paul Jose Yacaman, Miguel |
| description | The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection. This need has generated a high number of new testing methods aimed at replacing RT-PCR, which is the golden standard for testing. Most of the testing techniques are based on biochemistry methods and require chemicals that are often expensive and the supply might become scarce in a large crisis. In the present paper we suggest the use of methods based on physics that leverage novel nanomaterials. We demonstrate that using Surface Enhanced Raman Spectroscopy (SERS) of virion particles a very distinct spectroscopic signature of the SARS-CoV-2 virus can be obtained. We demonstrate that the spectra are mainly composed by signals from the spike (S) and nucleocapsid (N) proteins. It is believed that a clinical test using SERS can be developed. The test will be fast, inexpensive, and reliable. It is also clear that SERS can be used for analysis of structural changes on the S and N proteins. This will be an example of application of nanotechnology and properties of nanoparticles for health and social related matters.
The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection. |
| doi_str_mv | 10.1039/d1ra03481b |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_rsc_p</sourceid><recordid>TN_cdi_rsc_primary_d1ra03481b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2656746548</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-873e7182fed5c89e457bb045f650f9cded094e4926f5ae896acc79d43b1aaabf3</originalsourceid><addsrcrecordid>eNpdkd9rFDEQx4MottS--K4EfBFhNb83eRHOq7aFonCnPvgSstlJu-UuWZNdof-9sVfP1nmZge-HLzPzReg5JW8p4eZdT7MjXGjaPUKHjAjVMKLM43vzATou5ZrUUpIyRZ-iAy5Fq7Xih-jHCUzgpyFFnAJeL1brZpm-Nwy72ONhKnh9O33GY04TDLHguQzxEpc5B-cBQ7xy0UOPV27rIi5jNcup-DTePENPgtsUOL7rR-jbp49fl2fNxZfT8-XiovFCqqnRLYeWahagl14bELLtOiJkUJIE43voiREgDFNBOtBGOe9b0wveUedcF_gRer_zHeduC72HOGW3sWMeti7f2OQG-1CJw5W9TL-sIbytP6wGr-8Mcvo5Q5nsdigeNhsXIc3FMiVVK5QUuqKv_kOv05xjPc8yWYtpyUml3uwoX19RMoT9MpTYP6nZE7pa3Kb2ocIv76-_R_9mVIEXOyAXv1f_xc5_AyGmm-o</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2555528530</pqid></control><display><type>article</type><title>Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Sanchez, John Eder ; Jaramillo, Sierra A ; Settles, Erik ; Velazquez Salazar, J. Jesus ; Lehr, Alexander ; Gonzalez, Javier ; Rodríguez Aranda, Carmen ; Navarro-Contreras, Hugo R ; Raniere, Marlee O ; Harvey, Maxx ; Wagner, David M ; Koppisch, Andrew ; Kellar, Robert ; Keim, Paul ; Jose Yacaman, Miguel</creator><creatorcontrib>Sanchez, John Eder ; Jaramillo, Sierra A ; Settles, Erik ; Velazquez Salazar, J. Jesus ; Lehr, Alexander ; Gonzalez, Javier ; Rodríguez Aranda, Carmen ; Navarro-Contreras, Hugo R ; Raniere, Marlee O ; Harvey, Maxx ; Wagner, David M ; Koppisch, Andrew ; Kellar, Robert ; Keim, Paul ; Jose Yacaman, Miguel</creatorcontrib><description>The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection. This need has generated a high number of new testing methods aimed at replacing RT-PCR, which is the golden standard for testing. Most of the testing techniques are based on biochemistry methods and require chemicals that are often expensive and the supply might become scarce in a large crisis. In the present paper we suggest the use of methods based on physics that leverage novel nanomaterials. We demonstrate that using Surface Enhanced Raman Spectroscopy (SERS) of virion particles a very distinct spectroscopic signature of the SARS-CoV-2 virus can be obtained. We demonstrate that the spectra are mainly composed by signals from the spike (S) and nucleocapsid (N) proteins. It is believed that a clinical test using SERS can be developed. The test will be fast, inexpensive, and reliable. It is also clear that SERS can be used for analysis of structural changes on the S and N proteins. This will be an example of application of nanotechnology and properties of nanoparticles for health and social related matters.
The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d1ra03481b</identifier><identifier>PMID: 35478863</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Chemistry ; COVID-19 ; Nanomaterials ; Nanoparticles ; Nanotechnology ; Proteins ; Raman spectroscopy ; Severe acute respiratory syndrome coronavirus 2 ; Spectrum analysis ; Viral diseases ; Viruses</subject><ispartof>RSC advances, 2021-07, Vol.11 (41), p.25788-25794</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2021</rights><rights>This journal is © The Royal Society of Chemistry 2021 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-873e7182fed5c89e457bb045f650f9cded094e4926f5ae896acc79d43b1aaabf3</citedby><cites>FETCH-LOGICAL-c456t-873e7182fed5c89e457bb045f650f9cded094e4926f5ae896acc79d43b1aaabf3</cites><orcidid>0000-0003-0632-2265 ; 0000-0003-0333-0024 ; 0000-0003-2684-6007 ; 0000-0002-2945-6509</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037103/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037103/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35478863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanchez, John Eder</creatorcontrib><creatorcontrib>Jaramillo, Sierra A</creatorcontrib><creatorcontrib>Settles, Erik</creatorcontrib><creatorcontrib>Velazquez Salazar, J. Jesus</creatorcontrib><creatorcontrib>Lehr, Alexander</creatorcontrib><creatorcontrib>Gonzalez, Javier</creatorcontrib><creatorcontrib>Rodríguez Aranda, Carmen</creatorcontrib><creatorcontrib>Navarro-Contreras, Hugo R</creatorcontrib><creatorcontrib>Raniere, Marlee O</creatorcontrib><creatorcontrib>Harvey, Maxx</creatorcontrib><creatorcontrib>Wagner, David M</creatorcontrib><creatorcontrib>Koppisch, Andrew</creatorcontrib><creatorcontrib>Kellar, Robert</creatorcontrib><creatorcontrib>Keim, Paul</creatorcontrib><creatorcontrib>Jose Yacaman, Miguel</creatorcontrib><title>Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection. This need has generated a high number of new testing methods aimed at replacing RT-PCR, which is the golden standard for testing. Most of the testing techniques are based on biochemistry methods and require chemicals that are often expensive and the supply might become scarce in a large crisis. In the present paper we suggest the use of methods based on physics that leverage novel nanomaterials. We demonstrate that using Surface Enhanced Raman Spectroscopy (SERS) of virion particles a very distinct spectroscopic signature of the SARS-CoV-2 virus can be obtained. We demonstrate that the spectra are mainly composed by signals from the spike (S) and nucleocapsid (N) proteins. It is believed that a clinical test using SERS can be developed. The test will be fast, inexpensive, and reliable. It is also clear that SERS can be used for analysis of structural changes on the S and N proteins. This will be an example of application of nanotechnology and properties of nanoparticles for health and social related matters.
The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection.</description><subject>Chemistry</subject><subject>COVID-19</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Proteins</subject><subject>Raman spectroscopy</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Spectrum analysis</subject><subject>Viral diseases</subject><subject>Viruses</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkd9rFDEQx4MottS--K4EfBFhNb83eRHOq7aFonCnPvgSstlJu-UuWZNdof-9sVfP1nmZge-HLzPzReg5JW8p4eZdT7MjXGjaPUKHjAjVMKLM43vzATou5ZrUUpIyRZ-iAy5Fq7Xih-jHCUzgpyFFnAJeL1brZpm-Nwy72ONhKnh9O33GY04TDLHguQzxEpc5B-cBQ7xy0UOPV27rIi5jNcup-DTePENPgtsUOL7rR-jbp49fl2fNxZfT8-XiovFCqqnRLYeWahagl14bELLtOiJkUJIE43voiREgDFNBOtBGOe9b0wveUedcF_gRer_zHeduC72HOGW3sWMeti7f2OQG-1CJw5W9TL-sIbytP6wGr-8Mcvo5Q5nsdigeNhsXIc3FMiVVK5QUuqKv_kOv05xjPc8yWYtpyUml3uwoX19RMoT9MpTYP6nZE7pa3Kb2ocIv76-_R_9mVIEXOyAXv1f_xc5_AyGmm-o</recordid><startdate>20210719</startdate><enddate>20210719</enddate><creator>Sanchez, John Eder</creator><creator>Jaramillo, Sierra A</creator><creator>Settles, Erik</creator><creator>Velazquez Salazar, J. Jesus</creator><creator>Lehr, Alexander</creator><creator>Gonzalez, Javier</creator><creator>Rodríguez Aranda, Carmen</creator><creator>Navarro-Contreras, Hugo R</creator><creator>Raniere, Marlee O</creator><creator>Harvey, Maxx</creator><creator>Wagner, David M</creator><creator>Koppisch, Andrew</creator><creator>Kellar, Robert</creator><creator>Keim, Paul</creator><creator>Jose Yacaman, Miguel</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0632-2265</orcidid><orcidid>https://orcid.org/0000-0003-0333-0024</orcidid><orcidid>https://orcid.org/0000-0003-2684-6007</orcidid><orcidid>https://orcid.org/0000-0002-2945-6509</orcidid></search><sort><creationdate>20210719</creationdate><title>Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy</title><author>Sanchez, John Eder ; Jaramillo, Sierra A ; Settles, Erik ; Velazquez Salazar, J. Jesus ; Lehr, Alexander ; Gonzalez, Javier ; Rodríguez Aranda, Carmen ; Navarro-Contreras, Hugo R ; Raniere, Marlee O ; Harvey, Maxx ; Wagner, David M ; Koppisch, Andrew ; Kellar, Robert ; Keim, Paul ; Jose Yacaman, Miguel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-873e7182fed5c89e457bb045f650f9cded094e4926f5ae896acc79d43b1aaabf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Chemistry</topic><topic>COVID-19</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Proteins</topic><topic>Raman spectroscopy</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Spectrum analysis</topic><topic>Viral diseases</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanchez, John Eder</creatorcontrib><creatorcontrib>Jaramillo, Sierra A</creatorcontrib><creatorcontrib>Settles, Erik</creatorcontrib><creatorcontrib>Velazquez Salazar, J. Jesus</creatorcontrib><creatorcontrib>Lehr, Alexander</creatorcontrib><creatorcontrib>Gonzalez, Javier</creatorcontrib><creatorcontrib>Rodríguez Aranda, Carmen</creatorcontrib><creatorcontrib>Navarro-Contreras, Hugo R</creatorcontrib><creatorcontrib>Raniere, Marlee O</creatorcontrib><creatorcontrib>Harvey, Maxx</creatorcontrib><creatorcontrib>Wagner, David M</creatorcontrib><creatorcontrib>Koppisch, Andrew</creatorcontrib><creatorcontrib>Kellar, Robert</creatorcontrib><creatorcontrib>Keim, Paul</creatorcontrib><creatorcontrib>Jose Yacaman, Miguel</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanchez, John Eder</au><au>Jaramillo, Sierra A</au><au>Settles, Erik</au><au>Velazquez Salazar, J. Jesus</au><au>Lehr, Alexander</au><au>Gonzalez, Javier</au><au>Rodríguez Aranda, Carmen</au><au>Navarro-Contreras, Hugo R</au><au>Raniere, Marlee O</au><au>Harvey, Maxx</au><au>Wagner, David M</au><au>Koppisch, Andrew</au><au>Kellar, Robert</au><au>Keim, Paul</au><au>Jose Yacaman, Miguel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2021-07-19</date><risdate>2021</risdate><volume>11</volume><issue>41</issue><spage>25788</spage><epage>25794</epage><pages>25788-25794</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection. This need has generated a high number of new testing methods aimed at replacing RT-PCR, which is the golden standard for testing. Most of the testing techniques are based on biochemistry methods and require chemicals that are often expensive and the supply might become scarce in a large crisis. In the present paper we suggest the use of methods based on physics that leverage novel nanomaterials. We demonstrate that using Surface Enhanced Raman Spectroscopy (SERS) of virion particles a very distinct spectroscopic signature of the SARS-CoV-2 virus can be obtained. We demonstrate that the spectra are mainly composed by signals from the spike (S) and nucleocapsid (N) proteins. It is believed that a clinical test using SERS can be developed. The test will be fast, inexpensive, and reliable. It is also clear that SERS can be used for analysis of structural changes on the S and N proteins. This will be an example of application of nanotechnology and properties of nanoparticles for health and social related matters.
The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35478863</pmid><doi>10.1039/d1ra03481b</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0632-2265</orcidid><orcidid>https://orcid.org/0000-0003-0333-0024</orcidid><orcidid>https://orcid.org/0000-0003-2684-6007</orcidid><orcidid>https://orcid.org/0000-0002-2945-6509</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2046-2069 |
| ispartof | RSC advances, 2021-07, Vol.11 (41), p.25788-25794 |
| issn | 2046-2069 2046-2069 |
| language | eng |
| recordid | cdi_rsc_primary_d1ra03481b |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Chemistry COVID-19 Nanomaterials Nanoparticles Nanotechnology Proteins Raman spectroscopy Severe acute respiratory syndrome coronavirus 2 Spectrum analysis Viral diseases Viruses |
| title | Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T03%3A20%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_rsc_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20SARS-CoV-2%20and%20its%20S%20and%20N%20proteins%20using%20surface%20enhanced%20Raman%20spectroscopy&rft.jtitle=RSC%20advances&rft.au=Sanchez,%20John%20Eder&rft.date=2021-07-19&rft.volume=11&rft.issue=41&rft.spage=25788&rft.epage=25794&rft.pages=25788-25794&rft.issn=2046-2069&rft.eissn=2046-2069&rft_id=info:doi/10.1039/d1ra03481b&rft_dat=%3Cproquest_rsc_p%3E2656746548%3C/proquest_rsc_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2555528530&rft_id=info:pmid/35478863&rfr_iscdi=true |