Synthesis of the pentasaccharide repeating unit from and measurement of its inflammatory properties
The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases. For example, blooms of the Gram-positive bacterium Ruminococcus gnavus have been correlated with inflammatory bowel disease,...
Gespeichert in:
| Veröffentlicht in: | RSC advances 2021-04, Vol.11 (24), p.14357-14361 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 14361 |
|---|---|
| container_issue | 24 |
| container_start_page | 14357 |
| container_title | RSC advances |
| container_volume | 11 |
| creator | Haynie, Teron Gubler, Shawn Drees, Christoph Heaton, Tanner Mitton, Tanner Gleave, Quinn Bendelac, Albert Deng, Shenglou Savage, Paul B |
| description | The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases. For example, blooms of the Gram-positive bacterium
Ruminococcus gnavus
have been correlated with inflammatory bowel disease, and recently a polysaccharide produced by this organism was shown to stimulate release of inflammatory cytokines. This stimulation was proposed to signal through toll-like receptor 4 (TLR4). We have synthesized the pentasaccharide repeating unit of this polysaccharide and showed that it stimulates TNF-α and IL-6 release from bone marrow-derived dendritic cells (BMDCs) in a TLR4-dependent manner. A related glycan does not stimulate significant cytokine release, demonstrating TLR4 selectivity in glycan recognition.
The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases. |
| doi_str_mv | 10.1039/d1ra01918j |
| format | Article |
| fullrecord | <record><control><sourceid>rsc</sourceid><recordid>TN_cdi_rsc_primary_d1ra01918j</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>d1ra01918j</sourcerecordid><originalsourceid>FETCH-rsc_primary_d1ra01918j3</originalsourceid><addsrcrecordid>eNqFjrEKwkAQRA9BUDSNvbA_EL2LGkwtir32YUk2eiF3OXbPIn9vBMHSaWbgzcAotTJ6Y_Su2NaGUZvCHNuJmmd6n6eZzouZSkRaPSo_mCw3c1XdBh-fJFagb2BMEMhHFKyqJ7KtCZgCYbT-AS9vIzTcO0BfgyOUF5Mb65-pjQLWNx06h7HnAQL3gThakqWaNtgJJV9fqPXlfD9dU5aqDGwd8lD-Du_-8Tfr8Uf5</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Synthesis of the pentasaccharide repeating unit from and measurement of its inflammatory properties</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><source>PubMed Central Open Access</source><creator>Haynie, Teron ; Gubler, Shawn ; Drees, Christoph ; Heaton, Tanner ; Mitton, Tanner ; Gleave, Quinn ; Bendelac, Albert ; Deng, Shenglou ; Savage, Paul B</creator><creatorcontrib>Haynie, Teron ; Gubler, Shawn ; Drees, Christoph ; Heaton, Tanner ; Mitton, Tanner ; Gleave, Quinn ; Bendelac, Albert ; Deng, Shenglou ; Savage, Paul B</creatorcontrib><description>The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases. For example, blooms of the Gram-positive bacterium
Ruminococcus gnavus
have been correlated with inflammatory bowel disease, and recently a polysaccharide produced by this organism was shown to stimulate release of inflammatory cytokines. This stimulation was proposed to signal through toll-like receptor 4 (TLR4). We have synthesized the pentasaccharide repeating unit of this polysaccharide and showed that it stimulates TNF-α and IL-6 release from bone marrow-derived dendritic cells (BMDCs) in a TLR4-dependent manner. A related glycan does not stimulate significant cytokine release, demonstrating TLR4 selectivity in glycan recognition.
The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases.</description><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d1ra01918j</identifier><ispartof>RSC advances, 2021-04, Vol.11 (24), p.14357-14361</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Haynie, Teron</creatorcontrib><creatorcontrib>Gubler, Shawn</creatorcontrib><creatorcontrib>Drees, Christoph</creatorcontrib><creatorcontrib>Heaton, Tanner</creatorcontrib><creatorcontrib>Mitton, Tanner</creatorcontrib><creatorcontrib>Gleave, Quinn</creatorcontrib><creatorcontrib>Bendelac, Albert</creatorcontrib><creatorcontrib>Deng, Shenglou</creatorcontrib><creatorcontrib>Savage, Paul B</creatorcontrib><title>Synthesis of the pentasaccharide repeating unit from and measurement of its inflammatory properties</title><title>RSC advances</title><description>The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases. For example, blooms of the Gram-positive bacterium
Ruminococcus gnavus
have been correlated with inflammatory bowel disease, and recently a polysaccharide produced by this organism was shown to stimulate release of inflammatory cytokines. This stimulation was proposed to signal through toll-like receptor 4 (TLR4). We have synthesized the pentasaccharide repeating unit of this polysaccharide and showed that it stimulates TNF-α and IL-6 release from bone marrow-derived dendritic cells (BMDCs) in a TLR4-dependent manner. A related glycan does not stimulate significant cytokine release, demonstrating TLR4 selectivity in glycan recognition.
The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases.</description><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFjrEKwkAQRA9BUDSNvbA_EL2LGkwtir32YUk2eiF3OXbPIn9vBMHSaWbgzcAotTJ6Y_Su2NaGUZvCHNuJmmd6n6eZzouZSkRaPSo_mCw3c1XdBh-fJFagb2BMEMhHFKyqJ7KtCZgCYbT-AS9vIzTcO0BfgyOUF5Mb65-pjQLWNx06h7HnAQL3gThakqWaNtgJJV9fqPXlfD9dU5aqDGwd8lD-Du_-8Tfr8Uf5</recordid><startdate>20210416</startdate><enddate>20210416</enddate><creator>Haynie, Teron</creator><creator>Gubler, Shawn</creator><creator>Drees, Christoph</creator><creator>Heaton, Tanner</creator><creator>Mitton, Tanner</creator><creator>Gleave, Quinn</creator><creator>Bendelac, Albert</creator><creator>Deng, Shenglou</creator><creator>Savage, Paul B</creator><scope/></search><sort><creationdate>20210416</creationdate><title>Synthesis of the pentasaccharide repeating unit from and measurement of its inflammatory properties</title><author>Haynie, Teron ; Gubler, Shawn ; Drees, Christoph ; Heaton, Tanner ; Mitton, Tanner ; Gleave, Quinn ; Bendelac, Albert ; Deng, Shenglou ; Savage, Paul B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-rsc_primary_d1ra01918j3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haynie, Teron</creatorcontrib><creatorcontrib>Gubler, Shawn</creatorcontrib><creatorcontrib>Drees, Christoph</creatorcontrib><creatorcontrib>Heaton, Tanner</creatorcontrib><creatorcontrib>Mitton, Tanner</creatorcontrib><creatorcontrib>Gleave, Quinn</creatorcontrib><creatorcontrib>Bendelac, Albert</creatorcontrib><creatorcontrib>Deng, Shenglou</creatorcontrib><creatorcontrib>Savage, Paul B</creatorcontrib><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haynie, Teron</au><au>Gubler, Shawn</au><au>Drees, Christoph</au><au>Heaton, Tanner</au><au>Mitton, Tanner</au><au>Gleave, Quinn</au><au>Bendelac, Albert</au><au>Deng, Shenglou</au><au>Savage, Paul B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of the pentasaccharide repeating unit from and measurement of its inflammatory properties</atitle><jtitle>RSC advances</jtitle><date>2021-04-16</date><risdate>2021</risdate><volume>11</volume><issue>24</issue><spage>14357</spage><epage>14361</epage><pages>14357-14361</pages><eissn>2046-2069</eissn><abstract>The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases. For example, blooms of the Gram-positive bacterium
Ruminococcus gnavus
have been correlated with inflammatory bowel disease, and recently a polysaccharide produced by this organism was shown to stimulate release of inflammatory cytokines. This stimulation was proposed to signal through toll-like receptor 4 (TLR4). We have synthesized the pentasaccharide repeating unit of this polysaccharide and showed that it stimulates TNF-α and IL-6 release from bone marrow-derived dendritic cells (BMDCs) in a TLR4-dependent manner. A related glycan does not stimulate significant cytokine release, demonstrating TLR4 selectivity in glycan recognition.
The roles played by the gut microbiome in human health are increasingly recognized, and the prevalence of specific microorganisms has been correlated with different diseases.</abstract><doi>10.1039/d1ra01918j</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 2046-2069 |
| ispartof | RSC advances, 2021-04, Vol.11 (24), p.14357-14361 |
| issn | 2046-2069 |
| language | |
| recordid | cdi_rsc_primary_d1ra01918j |
| source | DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library; PubMed Central Open Access |
| title | Synthesis of the pentasaccharide repeating unit from and measurement of its inflammatory properties |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T21%3A46%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-rsc&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synthesis%20of%20the%20pentasaccharide%20repeating%20unit%20from%20and%20measurement%20of%20its%20inflammatory%20properties&rft.jtitle=RSC%20advances&rft.au=Haynie,%20Teron&rft.date=2021-04-16&rft.volume=11&rft.issue=24&rft.spage=14357&rft.epage=14361&rft.pages=14357-14361&rft.eissn=2046-2069&rft_id=info:doi/10.1039/d1ra01918j&rft_dat=%3Crsc%3Ed1ra01918j%3C/rsc%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |