Detection of lipase activity in human serum based on a ratiometric fluorescent probe
Acute pancreatitis results in major mortality and morbidity. Lipase is a vital biomarker of acute pancreatitis in the early stage. Developing a simple and convenient methodology for monitoring the lipase activity is of significance to the early diagnosis of acute pancreatitis. In this work, we desig...
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| Veröffentlicht in: | New journal of chemistry 2021-06, Vol.45 (21), p.9561-9568 |
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| container_end_page | 9568 |
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| container_issue | 21 |
| container_start_page | 9561 |
| container_title | New journal of chemistry |
| container_volume | 45 |
| creator | Luo, Jiajie Zhang, Hongyi Guan, Jialiang An, Baoshuai Peng, Junli Zhu, Wei Wei, Ningning Zhang, Yanru |
| description | Acute pancreatitis results in major mortality and morbidity. Lipase is a vital biomarker of acute pancreatitis in the early stage. Developing a simple and convenient methodology for monitoring the lipase activity is of significance to the early diagnosis of acute pancreatitis. In this work, we designed a novel ratiometric fluorescent probe (CARA) based on FRET for detecting lipase activity in human serum. The energy donor group coumarin derivative (CA) and the energy acceptor group rhodamine derivative (RA) were linked for the construction of a coumarin-rhodamine platform through an ester bond that is easily cleaved by lipase, breaking the FRET process between CA and RA. CARA shows a high selectivity to lipase over other biological substances and a good linearity between the fluorescence ratio and lipase activity in serum samples. CARA has the potential to be applied in the clinical diagnosis of acute pancreatitis.
CARA can monitor lipase activity through hydrolyzing the ester bond to interrupt the FRET process. |
| doi_str_mv | 10.1039/d1nj01155c |
| format | Article |
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CARA can monitor lipase activity through hydrolyzing the ester bond to interrupt the FRET process.</description><subject>Biomarkers</subject><subject>Coumarin</subject><subject>Diagnosis</subject><subject>Fluorescent indicators</subject><subject>Lipase</subject><subject>Pancreatitis</subject><subject>Rhodamine</subject><subject>Selectivity</subject><issn>1144-0546</issn><issn>1369-9261</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpFkEtLAzEQgIMoWKsX70LAm7CaSTZp9yhtfVH0Us_LbHaCW7q7NckK_femVvQ0r29m4GPsEsQtCFXc1dCtBYDW9oiNQJkiK6SB45RDnmdC5-aUnYWwFgmaGBix1Zwi2dj0He8d3zRbDMQxNb6auONNxz-GFjseyA8tr9Kw5glF7jHttBR9Y7nbDL2nYKmLfOv7is7ZicNNoIvfOGbvD4vV7Clbvj0-z-6XmVVSxkxOhCkcyrzASkhtlS5chQhgkWxdJya3lTEESuLU1VrWuaoAplMCp0Uqxuz6cDd9_RwoxHLdD75LL0uplQaVlOypmwNlfR-CJ1dufdOi35Ugyr21cg6vLz_WZgm-OsA-2D_u36r6BvOQaZM</recordid><startdate>20210607</startdate><enddate>20210607</enddate><creator>Luo, Jiajie</creator><creator>Zhang, Hongyi</creator><creator>Guan, Jialiang</creator><creator>An, Baoshuai</creator><creator>Peng, Junli</creator><creator>Zhu, Wei</creator><creator>Wei, Ningning</creator><creator>Zhang, Yanru</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>H9R</scope><scope>JG9</scope><scope>KA0</scope><orcidid>https://orcid.org/0000-0002-5222-1919</orcidid></search><sort><creationdate>20210607</creationdate><title>Detection of lipase activity in human serum based on a ratiometric fluorescent probe</title><author>Luo, Jiajie ; Zhang, Hongyi ; Guan, Jialiang ; An, Baoshuai ; Peng, Junli ; Zhu, Wei ; Wei, Ningning ; Zhang, Yanru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c322t-27069fa249ab025c359fbaa11caecdd3224cb66e132a8fd52d43b1188e1f50d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomarkers</topic><topic>Coumarin</topic><topic>Diagnosis</topic><topic>Fluorescent indicators</topic><topic>Lipase</topic><topic>Pancreatitis</topic><topic>Rhodamine</topic><topic>Selectivity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Jiajie</creatorcontrib><creatorcontrib>Zhang, Hongyi</creatorcontrib><creatorcontrib>Guan, Jialiang</creatorcontrib><creatorcontrib>An, Baoshuai</creatorcontrib><creatorcontrib>Peng, Junli</creatorcontrib><creatorcontrib>Zhu, Wei</creatorcontrib><creatorcontrib>Wei, Ningning</creatorcontrib><creatorcontrib>Zhang, Yanru</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Illustrata: Natural Sciences</collection><collection>Materials Research Database</collection><collection>ProQuest Illustrata: Technology Collection</collection><jtitle>New journal of chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Jiajie</au><au>Zhang, Hongyi</au><au>Guan, Jialiang</au><au>An, Baoshuai</au><au>Peng, Junli</au><au>Zhu, Wei</au><au>Wei, Ningning</au><au>Zhang, Yanru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of lipase activity in human serum based on a ratiometric fluorescent probe</atitle><jtitle>New journal of chemistry</jtitle><date>2021-06-07</date><risdate>2021</risdate><volume>45</volume><issue>21</issue><spage>9561</spage><epage>9568</epage><pages>9561-9568</pages><issn>1144-0546</issn><eissn>1369-9261</eissn><abstract>Acute pancreatitis results in major mortality and morbidity. Lipase is a vital biomarker of acute pancreatitis in the early stage. Developing a simple and convenient methodology for monitoring the lipase activity is of significance to the early diagnosis of acute pancreatitis. In this work, we designed a novel ratiometric fluorescent probe (CARA) based on FRET for detecting lipase activity in human serum. The energy donor group coumarin derivative (CA) and the energy acceptor group rhodamine derivative (RA) were linked for the construction of a coumarin-rhodamine platform through an ester bond that is easily cleaved by lipase, breaking the FRET process between CA and RA. CARA shows a high selectivity to lipase over other biological substances and a good linearity between the fluorescence ratio and lipase activity in serum samples. CARA has the potential to be applied in the clinical diagnosis of acute pancreatitis.
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| ispartof | New journal of chemistry, 2021-06, Vol.45 (21), p.9561-9568 |
| issn | 1144-0546 1369-9261 |
| language | eng |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Biomarkers Coumarin Diagnosis Fluorescent indicators Lipase Pancreatitis Rhodamine Selectivity |
| title | Detection of lipase activity in human serum based on a ratiometric fluorescent probe |
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