Small molecule-induced trinucleotide repeat contractions during DNA synthesis

Small molecule-induced trinucleotide repeat contractions during DNA synthesis

We demonstrated that a synthetic ligand NA, which selectively binds to a 5′-CAG-3′/5′-CAG-3′ triad, induced repeat contractions during DNA polymerase-mediated primer extension through the CAG repeat template. A thorough capillary electrophoresis and sequencing analysis revealed that the d(CAG) 20 te...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2021-03, Vol.57 (26), p.3235-3238
Hauptverfasser: Dohno, Chikara, Hagihara, Masaki, Binti Mohd Zaifuddin, Nursakinah, Nihei, Mizuki, Saito, Kaoru, Nakatani, Kazuhiko
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container_issue 26
container_start_page 3235
container_title Chemical communications (Cambridge, England)
container_volume 57
creator Dohno, Chikara
Hagihara, Masaki
Binti Mohd Zaifuddin, Nursakinah
Nihei, Mizuki
Saito, Kaoru
Nakatani, Kazuhiko
description We demonstrated that a synthetic ligand NA, which selectively binds to a 5′-CAG-3′/5′-CAG-3′ triad, induced repeat contractions during DNA polymerase-mediated primer extension through the CAG repeat template. A thorough capillary electrophoresis and sequencing analysis revealed that the d(CAG) 20 template gave shortened nascent strands mainly containing 3-6 CTG units in the presence of NA. A CAG repeat binding molecule, NA, induced efficient repeat contractions during DNA polymerase-mediated primer extension through a CAG repeat template.
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title Small molecule-induced trinucleotide repeat contractions during DNA synthesis
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