Simultaneous determination of four fertility-related hormones in saliva using disposable multiplexed immunoplatforms coupled to a custom-designed and field-portable potentiostat
This work reports the first amperometric immunosensor for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL). The immune platform involves direct competitive (P4 and E2), and sandwich (LH and P...
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| Veröffentlicht in: | Analytical methods 2021-08, Vol.13 (31), p.3471-3478 |
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| creator | Arévalo, Beatriz Serafín, Verónica Beltrán-Sánchez, José Francisco Aznar-Poveda, Juan López-Pastor, José Antonio García-Sánchez, Antonio Javier García-Haro, Joan Campuzano, Susana Yañez-Sedeño, Paloma Pingarrón, José M |
| description | This work reports the first amperometric immunosensor for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL). The immune platform involves direct competitive (P4 and E2), and sandwich (LH and PRL) assays implemented onto functionalized magnetic microbeads (MBs). The amperometric transduction was performed upon placing the MBs-immunoconjugates onto each of the four working electrodes of the SPCE array (SP
4
CEs) and applying a detection potential of −0.20 V (
vs.
Ag pseudo-reference electrode) using the H
2
O
2
/hydroquinone (HQ) system. The achieved analytical and operational characteristics of the developed multiplexed immunoplatform showed a sensitivity that allows the determination of these hormones in saliva, and an adequate selectivity to analyse complex clinical samples. The bioplatform was employed for the determination of the set of four hormones in human saliva samples collected from individuals with different hormonal profiles. The results obtained using a conventional potentiostat were compared with those provided employing a novel low-cost custom-designed and field-portable quadruple potentiostat. Similar results were found which also agreed with those obtained by applying ELISA methods for the determination of single hormones.
This work reports the first amperometric immunoplatform for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL) using a custom-designed and field-portable quadruple potentiostat. |
| doi_str_mv | 10.1039/d1ay01074c |
| format | Article |
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4
CEs) and applying a detection potential of −0.20 V (
vs.
Ag pseudo-reference electrode) using the H
2
O
2
/hydroquinone (HQ) system. The achieved analytical and operational characteristics of the developed multiplexed immunoplatform showed a sensitivity that allows the determination of these hormones in saliva, and an adequate selectivity to analyse complex clinical samples. The bioplatform was employed for the determination of the set of four hormones in human saliva samples collected from individuals with different hormonal profiles. The results obtained using a conventional potentiostat were compared with those provided employing a novel low-cost custom-designed and field-portable quadruple potentiostat. Similar results were found which also agreed with those obtained by applying ELISA methods for the determination of single hormones.
This work reports the first amperometric immunoplatform for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL) using a custom-designed and field-portable quadruple potentiostat.</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/d1ay01074c</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>17β-Estradiol ; Customization ; Electrical measurement ; Electrodes ; Enzyme-linked immunosorbent assay ; Fertility ; Hormones ; Hydrogen peroxide ; Hydroquinone ; Immunoconjugates ; Immunosensors ; Luteinizing hormone ; Microspheres ; Multiplexing ; Nanoparticles ; Progesterone ; Prolactin ; Saliva ; Selectivity ; Sex hormones</subject><ispartof>Analytical methods, 2021-08, Vol.13 (31), p.3471-3478</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-99a5a37b6fdbecde3c4e0baf19b6c843ebc9276b27cb638ff9da2f7408742c7f3</citedby><cites>FETCH-LOGICAL-c314t-99a5a37b6fdbecde3c4e0baf19b6c843ebc9276b27cb638ff9da2f7408742c7f3</cites><orcidid>0000-0002-2644-8925 ; 0000-0001-8266-8669 ; 0000-0002-9928-6613 ; 0000-0003-0034-8456 ; 0000-0001-5095-3035 ; 0000-0002-1232-2723 ; 0000-0003-0741-7530 ; 0000-0002-0879-6651 ; 0000-0003-2271-1383</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Arévalo, Beatriz</creatorcontrib><creatorcontrib>Serafín, Verónica</creatorcontrib><creatorcontrib>Beltrán-Sánchez, José Francisco</creatorcontrib><creatorcontrib>Aznar-Poveda, Juan</creatorcontrib><creatorcontrib>López-Pastor, José Antonio</creatorcontrib><creatorcontrib>García-Sánchez, Antonio Javier</creatorcontrib><creatorcontrib>García-Haro, Joan</creatorcontrib><creatorcontrib>Campuzano, Susana</creatorcontrib><creatorcontrib>Yañez-Sedeño, Paloma</creatorcontrib><creatorcontrib>Pingarrón, José M</creatorcontrib><title>Simultaneous determination of four fertility-related hormones in saliva using disposable multiplexed immunoplatforms coupled to a custom-designed and field-portable potentiostat</title><title>Analytical methods</title><description>This work reports the first amperometric immunosensor for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL). The immune platform involves direct competitive (P4 and E2), and sandwich (LH and PRL) assays implemented onto functionalized magnetic microbeads (MBs). The amperometric transduction was performed upon placing the MBs-immunoconjugates onto each of the four working electrodes of the SPCE array (SP
4
CEs) and applying a detection potential of −0.20 V (
vs.
Ag pseudo-reference electrode) using the H
2
O
2
/hydroquinone (HQ) system. The achieved analytical and operational characteristics of the developed multiplexed immunoplatform showed a sensitivity that allows the determination of these hormones in saliva, and an adequate selectivity to analyse complex clinical samples. The bioplatform was employed for the determination of the set of four hormones in human saliva samples collected from individuals with different hormonal profiles. The results obtained using a conventional potentiostat were compared with those provided employing a novel low-cost custom-designed and field-portable quadruple potentiostat. Similar results were found which also agreed with those obtained by applying ELISA methods for the determination of single hormones.
This work reports the first amperometric immunoplatform for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL) using a custom-designed and field-portable quadruple potentiostat.</description><subject>17β-Estradiol</subject><subject>Customization</subject><subject>Electrical measurement</subject><subject>Electrodes</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Fertility</subject><subject>Hormones</subject><subject>Hydrogen peroxide</subject><subject>Hydroquinone</subject><subject>Immunoconjugates</subject><subject>Immunosensors</subject><subject>Luteinizing hormone</subject><subject>Microspheres</subject><subject>Multiplexing</subject><subject>Nanoparticles</subject><subject>Progesterone</subject><subject>Prolactin</subject><subject>Saliva</subject><subject>Selectivity</subject><subject>Sex hormones</subject><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc1q3TAQhU1ooGmSTfcBQTel4ESyfCVrGW5_IZBF2kVWRpZGqYIsuRo59D5W3rBKbkmhqxlmvjkc5jTNW0bPGeXqwjK9o4zK3hw0R0xuVKuEVK9eekFfN28Q7ykVigt21Dze-HkNRUdIKxILBfLsoy4-RZIccWnNxEEuPviyazMEXcCSnynPKQISHwnq4B80WdHHO2I9Lgn1FIA8yfolwO_K-3leY1rqsauXSExa68aSkogmZsWS5tYC-rtYhzpa4jwE2y4pl2etJRWI1RMWXU6aQ6cDwunfetz8-Pzp-_Zre3X95dv28qo1nPWlVUpvNJeTcHYCY4GbHuikHVOTMEPPYTKqk2LqpJkEH5xTVndO9nSQfWek48fN-73uktOvFbCMs0cDIex_NXabDR-YomKo6Lv_0Pv6t1jdVUrQTnFJZaU-7CmTE2IGNy7ZzzrvRkbHp_TGj-zy9jm9bYXP9nBG88L9S5f_AX1sncU</recordid><startdate>20210812</startdate><enddate>20210812</enddate><creator>Arévalo, Beatriz</creator><creator>Serafín, Verónica</creator><creator>Beltrán-Sánchez, José Francisco</creator><creator>Aznar-Poveda, Juan</creator><creator>López-Pastor, José Antonio</creator><creator>García-Sánchez, Antonio Javier</creator><creator>García-Haro, Joan</creator><creator>Campuzano, Susana</creator><creator>Yañez-Sedeño, Paloma</creator><creator>Pingarrón, José M</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2644-8925</orcidid><orcidid>https://orcid.org/0000-0001-8266-8669</orcidid><orcidid>https://orcid.org/0000-0002-9928-6613</orcidid><orcidid>https://orcid.org/0000-0003-0034-8456</orcidid><orcidid>https://orcid.org/0000-0001-5095-3035</orcidid><orcidid>https://orcid.org/0000-0002-1232-2723</orcidid><orcidid>https://orcid.org/0000-0003-0741-7530</orcidid><orcidid>https://orcid.org/0000-0002-0879-6651</orcidid><orcidid>https://orcid.org/0000-0003-2271-1383</orcidid></search><sort><creationdate>20210812</creationdate><title>Simultaneous determination of four fertility-related hormones in saliva using disposable multiplexed immunoplatforms coupled to a custom-designed and field-portable potentiostat</title><author>Arévalo, Beatriz ; Serafín, Verónica ; Beltrán-Sánchez, José Francisco ; Aznar-Poveda, Juan ; López-Pastor, José Antonio ; García-Sánchez, Antonio Javier ; García-Haro, Joan ; Campuzano, Susana ; Yañez-Sedeño, Paloma ; Pingarrón, José M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-99a5a37b6fdbecde3c4e0baf19b6c843ebc9276b27cb638ff9da2f7408742c7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>17β-Estradiol</topic><topic>Customization</topic><topic>Electrical measurement</topic><topic>Electrodes</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Fertility</topic><topic>Hormones</topic><topic>Hydrogen peroxide</topic><topic>Hydroquinone</topic><topic>Immunoconjugates</topic><topic>Immunosensors</topic><topic>Luteinizing hormone</topic><topic>Microspheres</topic><topic>Multiplexing</topic><topic>Nanoparticles</topic><topic>Progesterone</topic><topic>Prolactin</topic><topic>Saliva</topic><topic>Selectivity</topic><topic>Sex hormones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arévalo, Beatriz</creatorcontrib><creatorcontrib>Serafín, Verónica</creatorcontrib><creatorcontrib>Beltrán-Sánchez, José Francisco</creatorcontrib><creatorcontrib>Aznar-Poveda, Juan</creatorcontrib><creatorcontrib>López-Pastor, José Antonio</creatorcontrib><creatorcontrib>García-Sánchez, Antonio Javier</creatorcontrib><creatorcontrib>García-Haro, Joan</creatorcontrib><creatorcontrib>Campuzano, Susana</creatorcontrib><creatorcontrib>Yañez-Sedeño, Paloma</creatorcontrib><creatorcontrib>Pingarrón, José M</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arévalo, Beatriz</au><au>Serafín, Verónica</au><au>Beltrán-Sánchez, José Francisco</au><au>Aznar-Poveda, Juan</au><au>López-Pastor, José Antonio</au><au>García-Sánchez, Antonio Javier</au><au>García-Haro, Joan</au><au>Campuzano, Susana</au><au>Yañez-Sedeño, Paloma</au><au>Pingarrón, José M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of four fertility-related hormones in saliva using disposable multiplexed immunoplatforms coupled to a custom-designed and field-portable potentiostat</atitle><jtitle>Analytical methods</jtitle><date>2021-08-12</date><risdate>2021</risdate><volume>13</volume><issue>31</issue><spage>3471</spage><epage>3478</epage><pages>3471-3478</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><abstract>This work reports the first amperometric immunosensor for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL). The immune platform involves direct competitive (P4 and E2), and sandwich (LH and PRL) assays implemented onto functionalized magnetic microbeads (MBs). The amperometric transduction was performed upon placing the MBs-immunoconjugates onto each of the four working electrodes of the SPCE array (SP
4
CEs) and applying a detection potential of −0.20 V (
vs.
Ag pseudo-reference electrode) using the H
2
O
2
/hydroquinone (HQ) system. The achieved analytical and operational characteristics of the developed multiplexed immunoplatform showed a sensitivity that allows the determination of these hormones in saliva, and an adequate selectivity to analyse complex clinical samples. The bioplatform was employed for the determination of the set of four hormones in human saliva samples collected from individuals with different hormonal profiles. The results obtained using a conventional potentiostat were compared with those provided employing a novel low-cost custom-designed and field-portable quadruple potentiostat. Similar results were found which also agreed with those obtained by applying ELISA methods for the determination of single hormones.
This work reports the first amperometric immunoplatform for the simultaneous determination of four fertility-related hormones in saliva: progesterone (P4), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL) using a custom-designed and field-portable quadruple potentiostat.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d1ay01074c</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2644-8925</orcidid><orcidid>https://orcid.org/0000-0001-8266-8669</orcidid><orcidid>https://orcid.org/0000-0002-9928-6613</orcidid><orcidid>https://orcid.org/0000-0003-0034-8456</orcidid><orcidid>https://orcid.org/0000-0001-5095-3035</orcidid><orcidid>https://orcid.org/0000-0002-1232-2723</orcidid><orcidid>https://orcid.org/0000-0003-0741-7530</orcidid><orcidid>https://orcid.org/0000-0002-0879-6651</orcidid><orcidid>https://orcid.org/0000-0003-2271-1383</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1759-9660 |
| ispartof | Analytical methods, 2021-08, Vol.13 (31), p.3471-3478 |
| issn | 1759-9660 1759-9679 |
| language | eng |
| recordid | cdi_rsc_primary_d1ay01074c |
| source | Royal Society Of Chemistry Journals 2008- |
| subjects | 17β-Estradiol Customization Electrical measurement Electrodes Enzyme-linked immunosorbent assay Fertility Hormones Hydrogen peroxide Hydroquinone Immunoconjugates Immunosensors Luteinizing hormone Microspheres Multiplexing Nanoparticles Progesterone Prolactin Saliva Selectivity Sex hormones |
| title | Simultaneous determination of four fertility-related hormones in saliva using disposable multiplexed immunoplatforms coupled to a custom-designed and field-portable potentiostat |
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