Sterilization effects of UV laser irradiation on spore viability, structure, and proteins
Bacillus spores are highly resistant to toxic chemicals and extreme environments. Because some Bacillus species threaten public health, spore inactivation techniques have been intensively investigated. We exposed Bacillus atrophaeus spores to a 266 nm Nd:YVO 4 laser at a laser power of 1 W and vario...
Gespeichert in:
| Veröffentlicht in: | Analyst (London) 2021-12, Vol.146 (24), p.7682-7692 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7692 |
|---|---|
| container_issue | 24 |
| container_start_page | 7682 |
| container_title | Analyst (London) |
| container_volume | 146 |
| creator | Nguyen, My-Chi Thi Nguyen, Huu-Quang Jang, Hanbyeol Noh, Sojung Lee, Seong-Yeon Jang, Kyoung-Soon Lee, Jaebeom Sohn, Youngku Yee, Kiju Jung, Heesoo Kim, Jeongkwon |
| description | Bacillus
spores are highly resistant to toxic chemicals and extreme environments. Because some
Bacillus
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
Bacillus atrophaeus
spores to a 266 nm Nd:YVO
4
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
Bacillus atrophaeus
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
Bacillus
spores. Our findings suggest that a UV laser is capable of sterilizing
Bacillus atrophaeus
spores.
A UV-C laser is capable of sterilizing
Bacillus atrophaeus
spores. |
| doi_str_mv | 10.1039/d1an01717a |
| format | Article |
| fullrecord | <record><control><sourceid>rsc</sourceid><recordid>TN_cdi_rsc_primary_d1an01717a</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>d1an01717a</sourcerecordid><originalsourceid>FETCH-rsc_primary_d1an01717a3</originalsourceid><addsrcrecordid>eNqFjskKwkAQRAdRMC4X70J_gNGZrHoWxbsLeAptMoGRmITuiRC_3oCCR6HgUTwKSoiZkksl_c0qU1hKFasYe8JRfhS4Yeit-8KRUvquF4XBUIyY711VMpSOuB6tJlOYF1pTlaDzXKeWocrhfIECWRMYIszMx3fhuiINT4O3bmbbBbClJrUN6QVgmUFNldWm5IkY5Fiwnn45FvP97rQ9uMRpUpN5ILXJ76__z78BtnZEgQ</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Sterilization effects of UV laser irradiation on spore viability, structure, and proteins</title><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Nguyen, My-Chi Thi ; Nguyen, Huu-Quang ; Jang, Hanbyeol ; Noh, Sojung ; Lee, Seong-Yeon ; Jang, Kyoung-Soon ; Lee, Jaebeom ; Sohn, Youngku ; Yee, Kiju ; Jung, Heesoo ; Kim, Jeongkwon</creator><creatorcontrib>Nguyen, My-Chi Thi ; Nguyen, Huu-Quang ; Jang, Hanbyeol ; Noh, Sojung ; Lee, Seong-Yeon ; Jang, Kyoung-Soon ; Lee, Jaebeom ; Sohn, Youngku ; Yee, Kiju ; Jung, Heesoo ; Kim, Jeongkwon</creatorcontrib><description>Bacillus
spores are highly resistant to toxic chemicals and extreme environments. Because some
Bacillus
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
Bacillus atrophaeus
spores to a 266 nm Nd:YVO
4
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
Bacillus atrophaeus
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
Bacillus
spores. Our findings suggest that a UV laser is capable of sterilizing
Bacillus atrophaeus
spores.
A UV-C laser is capable of sterilizing
Bacillus atrophaeus
spores.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d1an01717a</identifier><ispartof>Analyst (London), 2021-12, Vol.146 (24), p.7682-7692</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2818,27903,27904</link.rule.ids></links><search><creatorcontrib>Nguyen, My-Chi Thi</creatorcontrib><creatorcontrib>Nguyen, Huu-Quang</creatorcontrib><creatorcontrib>Jang, Hanbyeol</creatorcontrib><creatorcontrib>Noh, Sojung</creatorcontrib><creatorcontrib>Lee, Seong-Yeon</creatorcontrib><creatorcontrib>Jang, Kyoung-Soon</creatorcontrib><creatorcontrib>Lee, Jaebeom</creatorcontrib><creatorcontrib>Sohn, Youngku</creatorcontrib><creatorcontrib>Yee, Kiju</creatorcontrib><creatorcontrib>Jung, Heesoo</creatorcontrib><creatorcontrib>Kim, Jeongkwon</creatorcontrib><title>Sterilization effects of UV laser irradiation on spore viability, structure, and proteins</title><title>Analyst (London)</title><description>Bacillus
spores are highly resistant to toxic chemicals and extreme environments. Because some
Bacillus
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
Bacillus atrophaeus
spores to a 266 nm Nd:YVO
4
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
Bacillus atrophaeus
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
Bacillus
spores. Our findings suggest that a UV laser is capable of sterilizing
Bacillus atrophaeus
spores.
A UV-C laser is capable of sterilizing
Bacillus atrophaeus
spores.</description><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFjskKwkAQRAdRMC4X70J_gNGZrHoWxbsLeAptMoGRmITuiRC_3oCCR6HgUTwKSoiZkksl_c0qU1hKFasYe8JRfhS4Yeit-8KRUvquF4XBUIyY711VMpSOuB6tJlOYF1pTlaDzXKeWocrhfIECWRMYIszMx3fhuiINT4O3bmbbBbClJrUN6QVgmUFNldWm5IkY5Fiwnn45FvP97rQ9uMRpUpN5ILXJ76__z78BtnZEgQ</recordid><startdate>20211206</startdate><enddate>20211206</enddate><creator>Nguyen, My-Chi Thi</creator><creator>Nguyen, Huu-Quang</creator><creator>Jang, Hanbyeol</creator><creator>Noh, Sojung</creator><creator>Lee, Seong-Yeon</creator><creator>Jang, Kyoung-Soon</creator><creator>Lee, Jaebeom</creator><creator>Sohn, Youngku</creator><creator>Yee, Kiju</creator><creator>Jung, Heesoo</creator><creator>Kim, Jeongkwon</creator><scope/></search><sort><creationdate>20211206</creationdate><title>Sterilization effects of UV laser irradiation on spore viability, structure, and proteins</title><author>Nguyen, My-Chi Thi ; Nguyen, Huu-Quang ; Jang, Hanbyeol ; Noh, Sojung ; Lee, Seong-Yeon ; Jang, Kyoung-Soon ; Lee, Jaebeom ; Sohn, Youngku ; Yee, Kiju ; Jung, Heesoo ; Kim, Jeongkwon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-rsc_primary_d1an01717a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><creationdate>2021</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, My-Chi Thi</creatorcontrib><creatorcontrib>Nguyen, Huu-Quang</creatorcontrib><creatorcontrib>Jang, Hanbyeol</creatorcontrib><creatorcontrib>Noh, Sojung</creatorcontrib><creatorcontrib>Lee, Seong-Yeon</creatorcontrib><creatorcontrib>Jang, Kyoung-Soon</creatorcontrib><creatorcontrib>Lee, Jaebeom</creatorcontrib><creatorcontrib>Sohn, Youngku</creatorcontrib><creatorcontrib>Yee, Kiju</creatorcontrib><creatorcontrib>Jung, Heesoo</creatorcontrib><creatorcontrib>Kim, Jeongkwon</creatorcontrib><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, My-Chi Thi</au><au>Nguyen, Huu-Quang</au><au>Jang, Hanbyeol</au><au>Noh, Sojung</au><au>Lee, Seong-Yeon</au><au>Jang, Kyoung-Soon</au><au>Lee, Jaebeom</au><au>Sohn, Youngku</au><au>Yee, Kiju</au><au>Jung, Heesoo</au><au>Kim, Jeongkwon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sterilization effects of UV laser irradiation on spore viability, structure, and proteins</atitle><jtitle>Analyst (London)</jtitle><date>2021-12-06</date><risdate>2021</risdate><volume>146</volume><issue>24</issue><spage>7682</spage><epage>7692</epage><pages>7682-7692</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Bacillus
spores are highly resistant to toxic chemicals and extreme environments. Because some
Bacillus
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
Bacillus atrophaeus
spores to a 266 nm Nd:YVO
4
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
Bacillus atrophaeus
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
Bacillus
spores. Our findings suggest that a UV laser is capable of sterilizing
Bacillus atrophaeus
spores.
A UV-C laser is capable of sterilizing
Bacillus atrophaeus
spores.</abstract><doi>10.1039/d1an01717a</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2021-12, Vol.146 (24), p.7682-7692 |
| issn | 0003-2654 1364-5528 |
| language | |
| recordid | cdi_rsc_primary_d1an01717a |
| source | Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| title | Sterilization effects of UV laser irradiation on spore viability, structure, and proteins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T03%3A40%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-rsc&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sterilization%20effects%20of%20UV%20laser%20irradiation%20on%20spore%20viability,%20structure,%20and%20proteins&rft.jtitle=Analyst%20(London)&rft.au=Nguyen,%20My-Chi%20Thi&rft.date=2021-12-06&rft.volume=146&rft.issue=24&rft.spage=7682&rft.epage=7692&rft.pages=7682-7692&rft.issn=0003-2654&rft.eissn=1364-5528&rft_id=info:doi/10.1039/d1an01717a&rft_dat=%3Crsc%3Ed1an01717a%3C/rsc%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |