Enrichment of cancer-initiating cells from colon cancer cells through porous polymeric membranes by a membrane filtration method
Cancer-initiating cells (CICs) or cancer stem cells (CSCs) are primarily responsible for tumor initiation, growth, and metastasis and represent a few percent of the total tumor cell population. We designed a membrane filtration protocol to enrich CICs (CSCs) from the LoVo colon cancer cell line via...
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| Veröffentlicht in: | Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2020-12, Vol.8 (46), p.1577-1585 |
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| creator | Sung, Tzu-Cheng Huang, Wei-Lun Ban, Lee-Kiat Lee, Henry Hsin-Chung Wang, Jia-Hua Su, Her-Young Jen, Shih Hsi Chang, Yen-Hsiang Yang, Jen-Ming Higuchi, Akon Ye, Qingsong |
| description | Cancer-initiating cells (CICs) or cancer stem cells (CSCs) are primarily responsible for tumor initiation, growth, and metastasis and represent a few percent of the total tumor cell population. We designed a membrane filtration protocol to enrich CICs (CSCs) from the LoVo colon cancer cell line
via
nylon mesh filter membranes with 11 and 20 μm pore sizes and poly(lactide-
co
-glycolic acid)/silk screen (PLGA/silk screen) porous membranes (pore sizes of 20-30 μm). The colon cancer cell solution was filtered through the membranes to obtain a permeate solution. Subsequently, the cell culture medium was filtered through the membranes to collect the recovery solution where the cells attached to the membranes were rinsed off into the recovery solution. Then, the membranes were cultivated in the cultivation medium to collect the migrated cells from the membranes. The cells migrated from any membrane had higher expression of the CSC surface markers CD44 and CD133, had higher colony formation levels, and produced more carcinoembryonic antigen (CEA) than the colon cancer cells cultivated on conventional tissue culture plates (control). We established a method to enrich the CICs (CSCs) of colon cancer cells from migrated cells through porous polymeric membranes by the membrane filtration protocol developed in this study.
A method to enrich the cancer stem cells of colon cancer cells through porous polymeric membranes is developed. |
| doi_str_mv | 10.1039/d0tb02312d |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_rsc_p</sourceid><recordid>TN_cdi_rsc_primary_d0tb02312d</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2467871874</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-c559ecfd4897111e6cfae1d0a9d9f235fa70fb0fb10d2d52bef520bd587c41613</originalsourceid><addsrcrecordid>eNpdkctLAzEYxIMoKtWLdyXgRYTVPDe7R23rAwQvCt6WbB7tymZTk_TQm3-60dYKhsB8SX4MEwaAE4yuMKL1tUapRYRionfAIUEcFYLjanc7o7cDcBzjO8qrwmVF2T44oJlnJaOH4HM6hE7NnRkS9BYqOSgTim7oUidTN8ygMn0foQ3eQeV7P2yQzX2aB7-czeHCZ41Z-pUz2RA649ogBxNhu4Jye4S261PIztnImTT3-gjsWdlHc7zREXi9m76MH4qn5_vH8c1ToRiiqVCc10ZZzapaYIxNqaw0WCNZ69oSyq0UyLZ5Y6SJ5qQ1lhPUal4JxXCJ6QhcrH0XwX8sTUyN6-L3J3KqHL0hjJcMCyJ4Rs__oe9-GYacLlOlqASuBMvU5ZpSwccYjG0WoXMyrBqMmu9qmgl6uf2pZpLhs43lsnVGb9HfIjJwugZCVNvXv27pF-3qlQc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2467871874</pqid></control><display><type>article</type><title>Enrichment of cancer-initiating cells from colon cancer cells through porous polymeric membranes by a membrane filtration method</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals</source><creator>Sung, Tzu-Cheng ; Huang, Wei-Lun ; Ban, Lee-Kiat ; Lee, Henry Hsin-Chung ; Wang, Jia-Hua ; Su, Her-Young ; Jen, Shih Hsi ; Chang, Yen-Hsiang ; Yang, Jen-Ming ; Higuchi, Akon ; Ye, Qingsong</creator><creatorcontrib>Sung, Tzu-Cheng ; Huang, Wei-Lun ; Ban, Lee-Kiat ; Lee, Henry Hsin-Chung ; Wang, Jia-Hua ; Su, Her-Young ; Jen, Shih Hsi ; Chang, Yen-Hsiang ; Yang, Jen-Ming ; Higuchi, Akon ; Ye, Qingsong</creatorcontrib><description>Cancer-initiating cells (CICs) or cancer stem cells (CSCs) are primarily responsible for tumor initiation, growth, and metastasis and represent a few percent of the total tumor cell population. We designed a membrane filtration protocol to enrich CICs (CSCs) from the LoVo colon cancer cell line
via
nylon mesh filter membranes with 11 and 20 μm pore sizes and poly(lactide-
co
-glycolic acid)/silk screen (PLGA/silk screen) porous membranes (pore sizes of 20-30 μm). The colon cancer cell solution was filtered through the membranes to obtain a permeate solution. Subsequently, the cell culture medium was filtered through the membranes to collect the recovery solution where the cells attached to the membranes were rinsed off into the recovery solution. Then, the membranes were cultivated in the cultivation medium to collect the migrated cells from the membranes. The cells migrated from any membrane had higher expression of the CSC surface markers CD44 and CD133, had higher colony formation levels, and produced more carcinoembryonic antigen (CEA) than the colon cancer cells cultivated on conventional tissue culture plates (control). We established a method to enrich the CICs (CSCs) of colon cancer cells from migrated cells through porous polymeric membranes by the membrane filtration protocol developed in this study.
A method to enrich the cancer stem cells of colon cancer cells through porous polymeric membranes is developed.</description><identifier>ISSN: 2050-750X</identifier><identifier>EISSN: 2050-7518</identifier><identifier>DOI: 10.1039/d0tb02312d</identifier><identifier>PMID: 33124643</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>AC133 Antigen - analysis ; AC133 Antigen - metabolism ; Antigens ; Cancer ; Carcinoembryonic antigen ; Carcinoembryonic Antigen - analysis ; Carcinoembryonic Antigen - metabolism ; CD44 antigen ; Cell culture ; Cell Line, Tumor ; Cell Separation - instrumentation ; Cell Separation - methods ; Colon ; Colon cancer ; Colonic Neoplasms - pathology ; Colorectal cancer ; Cultivation ; Enrichment ; Filtration ; Filtration - instrumentation ; Filtration - methods ; Glycolic acid ; Humans ; Hyaluronan Receptors - analysis ; Hyaluronan Receptors - metabolism ; Membrane filtration ; Membranes ; Membranes, Artificial ; Metastases ; Neoplastic Stem Cells - cytology ; Nylons - chemistry ; Polylactic Acid-Polyglycolic Acid Copolymer - chemistry ; Polylactide-co-glycolide ; Porosity ; Recovery ; Silk ; Silk - chemistry ; Stem cells ; Surface markers ; Tissue culture ; Tumors</subject><ispartof>Journal of materials chemistry. B, Materials for biology and medicine, 2020-12, Vol.8 (46), p.1577-1585</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-c559ecfd4897111e6cfae1d0a9d9f235fa70fb0fb10d2d52bef520bd587c41613</citedby><cites>FETCH-LOGICAL-c403t-c559ecfd4897111e6cfae1d0a9d9f235fa70fb0fb10d2d52bef520bd587c41613</cites><orcidid>0000-0003-2970-8531 ; 0000-0002-2868-9247</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33124643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sung, Tzu-Cheng</creatorcontrib><creatorcontrib>Huang, Wei-Lun</creatorcontrib><creatorcontrib>Ban, Lee-Kiat</creatorcontrib><creatorcontrib>Lee, Henry Hsin-Chung</creatorcontrib><creatorcontrib>Wang, Jia-Hua</creatorcontrib><creatorcontrib>Su, Her-Young</creatorcontrib><creatorcontrib>Jen, Shih Hsi</creatorcontrib><creatorcontrib>Chang, Yen-Hsiang</creatorcontrib><creatorcontrib>Yang, Jen-Ming</creatorcontrib><creatorcontrib>Higuchi, Akon</creatorcontrib><creatorcontrib>Ye, Qingsong</creatorcontrib><title>Enrichment of cancer-initiating cells from colon cancer cells through porous polymeric membranes by a membrane filtration method</title><title>Journal of materials chemistry. B, Materials for biology and medicine</title><addtitle>J Mater Chem B</addtitle><description>Cancer-initiating cells (CICs) or cancer stem cells (CSCs) are primarily responsible for tumor initiation, growth, and metastasis and represent a few percent of the total tumor cell population. We designed a membrane filtration protocol to enrich CICs (CSCs) from the LoVo colon cancer cell line
via
nylon mesh filter membranes with 11 and 20 μm pore sizes and poly(lactide-
co
-glycolic acid)/silk screen (PLGA/silk screen) porous membranes (pore sizes of 20-30 μm). The colon cancer cell solution was filtered through the membranes to obtain a permeate solution. Subsequently, the cell culture medium was filtered through the membranes to collect the recovery solution where the cells attached to the membranes were rinsed off into the recovery solution. Then, the membranes were cultivated in the cultivation medium to collect the migrated cells from the membranes. The cells migrated from any membrane had higher expression of the CSC surface markers CD44 and CD133, had higher colony formation levels, and produced more carcinoembryonic antigen (CEA) than the colon cancer cells cultivated on conventional tissue culture plates (control). We established a method to enrich the CICs (CSCs) of colon cancer cells from migrated cells through porous polymeric membranes by the membrane filtration protocol developed in this study.
A method to enrich the cancer stem cells of colon cancer cells through porous polymeric membranes is developed.</description><subject>AC133 Antigen - analysis</subject><subject>AC133 Antigen - metabolism</subject><subject>Antigens</subject><subject>Cancer</subject><subject>Carcinoembryonic antigen</subject><subject>Carcinoembryonic Antigen - analysis</subject><subject>Carcinoembryonic Antigen - metabolism</subject><subject>CD44 antigen</subject><subject>Cell culture</subject><subject>Cell Line, Tumor</subject><subject>Cell Separation - instrumentation</subject><subject>Cell Separation - methods</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colorectal cancer</subject><subject>Cultivation</subject><subject>Enrichment</subject><subject>Filtration</subject><subject>Filtration - instrumentation</subject><subject>Filtration - methods</subject><subject>Glycolic acid</subject><subject>Humans</subject><subject>Hyaluronan Receptors - analysis</subject><subject>Hyaluronan Receptors - metabolism</subject><subject>Membrane filtration</subject><subject>Membranes</subject><subject>Membranes, Artificial</subject><subject>Metastases</subject><subject>Neoplastic Stem Cells - cytology</subject><subject>Nylons - chemistry</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer - chemistry</subject><subject>Polylactide-co-glycolide</subject><subject>Porosity</subject><subject>Recovery</subject><subject>Silk</subject><subject>Silk - chemistry</subject><subject>Stem cells</subject><subject>Surface markers</subject><subject>Tissue culture</subject><subject>Tumors</subject><issn>2050-750X</issn><issn>2050-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkctLAzEYxIMoKtWLdyXgRYTVPDe7R23rAwQvCt6WbB7tymZTk_TQm3-60dYKhsB8SX4MEwaAE4yuMKL1tUapRYRionfAIUEcFYLjanc7o7cDcBzjO8qrwmVF2T44oJlnJaOH4HM6hE7NnRkS9BYqOSgTim7oUidTN8ygMn0foQ3eQeV7P2yQzX2aB7-czeHCZ41Z-pUz2RA649ogBxNhu4Jye4S261PIztnImTT3-gjsWdlHc7zREXi9m76MH4qn5_vH8c1ToRiiqVCc10ZZzapaYIxNqaw0WCNZ69oSyq0UyLZ5Y6SJ5qQ1lhPUal4JxXCJ6QhcrH0XwX8sTUyN6-L3J3KqHL0hjJcMCyJ4Rs__oe9-GYacLlOlqASuBMvU5ZpSwccYjG0WoXMyrBqMmu9qmgl6uf2pZpLhs43lsnVGb9HfIjJwugZCVNvXv27pF-3qlQc</recordid><startdate>20201208</startdate><enddate>20201208</enddate><creator>Sung, Tzu-Cheng</creator><creator>Huang, Wei-Lun</creator><creator>Ban, Lee-Kiat</creator><creator>Lee, Henry Hsin-Chung</creator><creator>Wang, Jia-Hua</creator><creator>Su, Her-Young</creator><creator>Jen, Shih Hsi</creator><creator>Chang, Yen-Hsiang</creator><creator>Yang, Jen-Ming</creator><creator>Higuchi, Akon</creator><creator>Ye, Qingsong</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2970-8531</orcidid><orcidid>https://orcid.org/0000-0002-2868-9247</orcidid></search><sort><creationdate>20201208</creationdate><title>Enrichment of cancer-initiating cells from colon cancer cells through porous polymeric membranes by a membrane filtration method</title><author>Sung, Tzu-Cheng ; Huang, Wei-Lun ; Ban, Lee-Kiat ; Lee, Henry Hsin-Chung ; Wang, Jia-Hua ; Su, Her-Young ; Jen, Shih Hsi ; Chang, Yen-Hsiang ; Yang, Jen-Ming ; Higuchi, Akon ; Ye, Qingsong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-c559ecfd4897111e6cfae1d0a9d9f235fa70fb0fb10d2d52bef520bd587c41613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>AC133 Antigen - analysis</topic><topic>AC133 Antigen - metabolism</topic><topic>Antigens</topic><topic>Cancer</topic><topic>Carcinoembryonic antigen</topic><topic>Carcinoembryonic Antigen - analysis</topic><topic>Carcinoembryonic Antigen - metabolism</topic><topic>CD44 antigen</topic><topic>Cell culture</topic><topic>Cell Line, Tumor</topic><topic>Cell Separation - instrumentation</topic><topic>Cell Separation - methods</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colorectal cancer</topic><topic>Cultivation</topic><topic>Enrichment</topic><topic>Filtration</topic><topic>Filtration - instrumentation</topic><topic>Filtration - methods</topic><topic>Glycolic acid</topic><topic>Humans</topic><topic>Hyaluronan Receptors - analysis</topic><topic>Hyaluronan Receptors - metabolism</topic><topic>Membrane filtration</topic><topic>Membranes</topic><topic>Membranes, Artificial</topic><topic>Metastases</topic><topic>Neoplastic Stem Cells - cytology</topic><topic>Nylons - chemistry</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer - chemistry</topic><topic>Polylactide-co-glycolide</topic><topic>Porosity</topic><topic>Recovery</topic><topic>Silk</topic><topic>Silk - chemistry</topic><topic>Stem cells</topic><topic>Surface markers</topic><topic>Tissue culture</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sung, Tzu-Cheng</creatorcontrib><creatorcontrib>Huang, Wei-Lun</creatorcontrib><creatorcontrib>Ban, Lee-Kiat</creatorcontrib><creatorcontrib>Lee, Henry Hsin-Chung</creatorcontrib><creatorcontrib>Wang, Jia-Hua</creatorcontrib><creatorcontrib>Su, Her-Young</creatorcontrib><creatorcontrib>Jen, Shih Hsi</creatorcontrib><creatorcontrib>Chang, Yen-Hsiang</creatorcontrib><creatorcontrib>Yang, Jen-Ming</creatorcontrib><creatorcontrib>Higuchi, Akon</creatorcontrib><creatorcontrib>Ye, Qingsong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sung, Tzu-Cheng</au><au>Huang, Wei-Lun</au><au>Ban, Lee-Kiat</au><au>Lee, Henry Hsin-Chung</au><au>Wang, Jia-Hua</au><au>Su, Her-Young</au><au>Jen, Shih Hsi</au><au>Chang, Yen-Hsiang</au><au>Yang, Jen-Ming</au><au>Higuchi, Akon</au><au>Ye, Qingsong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enrichment of cancer-initiating cells from colon cancer cells through porous polymeric membranes by a membrane filtration method</atitle><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle><addtitle>J Mater Chem B</addtitle><date>2020-12-08</date><risdate>2020</risdate><volume>8</volume><issue>46</issue><spage>1577</spage><epage>1585</epage><pages>1577-1585</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><abstract>Cancer-initiating cells (CICs) or cancer stem cells (CSCs) are primarily responsible for tumor initiation, growth, and metastasis and represent a few percent of the total tumor cell population. We designed a membrane filtration protocol to enrich CICs (CSCs) from the LoVo colon cancer cell line
via
nylon mesh filter membranes with 11 and 20 μm pore sizes and poly(lactide-
co
-glycolic acid)/silk screen (PLGA/silk screen) porous membranes (pore sizes of 20-30 μm). The colon cancer cell solution was filtered through the membranes to obtain a permeate solution. Subsequently, the cell culture medium was filtered through the membranes to collect the recovery solution where the cells attached to the membranes were rinsed off into the recovery solution. Then, the membranes were cultivated in the cultivation medium to collect the migrated cells from the membranes. The cells migrated from any membrane had higher expression of the CSC surface markers CD44 and CD133, had higher colony formation levels, and produced more carcinoembryonic antigen (CEA) than the colon cancer cells cultivated on conventional tissue culture plates (control). We established a method to enrich the CICs (CSCs) of colon cancer cells from migrated cells through porous polymeric membranes by the membrane filtration protocol developed in this study.
A method to enrich the cancer stem cells of colon cancer cells through porous polymeric membranes is developed.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>33124643</pmid><doi>10.1039/d0tb02312d</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2970-8531</orcidid><orcidid>https://orcid.org/0000-0002-2868-9247</orcidid></addata></record> |
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| ispartof | Journal of materials chemistry. B, Materials for biology and medicine, 2020-12, Vol.8 (46), p.1577-1585 |
| issn | 2050-750X 2050-7518 |
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| source | MEDLINE; Royal Society Of Chemistry Journals |
| subjects | AC133 Antigen - analysis AC133 Antigen - metabolism Antigens Cancer Carcinoembryonic antigen Carcinoembryonic Antigen - analysis Carcinoembryonic Antigen - metabolism CD44 antigen Cell culture Cell Line, Tumor Cell Separation - instrumentation Cell Separation - methods Colon Colon cancer Colonic Neoplasms - pathology Colorectal cancer Cultivation Enrichment Filtration Filtration - instrumentation Filtration - methods Glycolic acid Humans Hyaluronan Receptors - analysis Hyaluronan Receptors - metabolism Membrane filtration Membranes Membranes, Artificial Metastases Neoplastic Stem Cells - cytology Nylons - chemistry Polylactic Acid-Polyglycolic Acid Copolymer - chemistry Polylactide-co-glycolide Porosity Recovery Silk Silk - chemistry Stem cells Surface markers Tissue culture Tumors |
| title | Enrichment of cancer-initiating cells from colon cancer cells through porous polymeric membranes by a membrane filtration method |
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