Amperometric galectin-3 immunosensor-based gold nanoparticle-functionalized graphitic carbon nitride nanosheets and core-shell Ti-MOF@COFs composites
Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk. In this study, a novel sensitive amperometric method for antigen GL-3 detection is developed based on gold nanoparticle-functional...
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| Veröffentlicht in: | Nanoscale 2020-10, Vol.12 (38), p.19824-19832 |
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| creator | Yola, Mehmet Lütfi Atar, Necip |
| description | Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk. In this study, a novel sensitive amperometric method for antigen GL-3 detection is developed based on gold nanoparticle-functionalized graphitic carbon nitride nanosheets (g-C
3
N
4
@Au NPs) as the sensor platform and Ti-based metal organic framework (Ti-MOF, NH
2
-MIL-125)@covalent organic frameworks (COFs) composite for the signal amplification. The Ti-MOF@COF composite not only facilitates the penetration of antibody proteins into pore channels, but also the highly stable antigen-antibody interactions. The prepared sensor platform and signal amplification material are characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) method, X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The amperometric technique is utilized to achieve antigen GL-3 detection in plasma samples. The immunosensor demonstrates a wide linearity range (0.0001-20.0 ng mL
−1
) and a low detection limit (0.025 pg mL
−1
). Finally, the prepared immunosensor shows high stability and selectivity under optimum conditions.
Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk. |
| doi_str_mv | 10.1039/d0nr05614f |
| format | Article |
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3
N
4
@Au NPs) as the sensor platform and Ti-based metal organic framework (Ti-MOF, NH
2
-MIL-125)@covalent organic frameworks (COFs) composite for the signal amplification. The Ti-MOF@COF composite not only facilitates the penetration of antibody proteins into pore channels, but also the highly stable antigen-antibody interactions. The prepared sensor platform and signal amplification material are characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) method, X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The amperometric technique is utilized to achieve antigen GL-3 detection in plasma samples. The immunosensor demonstrates a wide linearity range (0.0001-20.0 ng mL
−1
) and a low detection limit (0.025 pg mL
−1
). Finally, the prepared immunosensor shows high stability and selectivity under optimum conditions.
Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/d0nr05614f</identifier><identifier>PMID: 32966539</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Amplification ; Antibodies ; Antigens ; Biomarkers ; Biosensing Techniques ; Carbon nitride ; Electrical measurement ; Electrochemical impedance spectroscopy ; Electrochemical Techniques ; Electron microscopy ; Fibrosis ; Fourier transforms ; Galactosides ; Galectin 3 ; Galectins ; Gold ; Graphite ; Immunoassay ; Immunosensors ; Limit of Detection ; Linearity ; Metal Nanoparticles ; Metal-Organic Frameworks ; Microscopy ; Nanoparticles ; Nanosheets ; Nitrogen Compounds ; Photoelectrons ; Proteins ; Selectivity ; Spectrum analysis ; Titanium ; X ray photoelectron spectroscopy</subject><ispartof>Nanoscale, 2020-10, Vol.12 (38), p.19824-19832</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-b9748ccfc0bc93ec770db53aba5caca6800ea9be7615183e7177d0f4c6643d3a3</citedby><cites>FETCH-LOGICAL-c429t-b9748ccfc0bc93ec770db53aba5caca6800ea9be7615183e7177d0f4c6643d3a3</cites><orcidid>0000-0001-7424-3425</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32966539$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yola, Mehmet Lütfi</creatorcontrib><creatorcontrib>Atar, Necip</creatorcontrib><title>Amperometric galectin-3 immunosensor-based gold nanoparticle-functionalized graphitic carbon nitride nanosheets and core-shell Ti-MOF@COFs composites</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk. In this study, a novel sensitive amperometric method for antigen GL-3 detection is developed based on gold nanoparticle-functionalized graphitic carbon nitride nanosheets (g-C
3
N
4
@Au NPs) as the sensor platform and Ti-based metal organic framework (Ti-MOF, NH
2
-MIL-125)@covalent organic frameworks (COFs) composite for the signal amplification. The Ti-MOF@COF composite not only facilitates the penetration of antibody proteins into pore channels, but also the highly stable antigen-antibody interactions. The prepared sensor platform and signal amplification material are characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) method, X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The amperometric technique is utilized to achieve antigen GL-3 detection in plasma samples. The immunosensor demonstrates a wide linearity range (0.0001-20.0 ng mL
−1
) and a low detection limit (0.025 pg mL
−1
). Finally, the prepared immunosensor shows high stability and selectivity under optimum conditions.
Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk.</description><subject>Amplification</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Biomarkers</subject><subject>Biosensing Techniques</subject><subject>Carbon nitride</subject><subject>Electrical measurement</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Electrochemical Techniques</subject><subject>Electron microscopy</subject><subject>Fibrosis</subject><subject>Fourier transforms</subject><subject>Galactosides</subject><subject>Galectin 3</subject><subject>Galectins</subject><subject>Gold</subject><subject>Graphite</subject><subject>Immunoassay</subject><subject>Immunosensors</subject><subject>Limit of Detection</subject><subject>Linearity</subject><subject>Metal Nanoparticles</subject><subject>Metal-Organic Frameworks</subject><subject>Microscopy</subject><subject>Nanoparticles</subject><subject>Nanosheets</subject><subject>Nitrogen Compounds</subject><subject>Photoelectrons</subject><subject>Proteins</subject><subject>Selectivity</subject><subject>Spectrum analysis</subject><subject>Titanium</subject><subject>X ray photoelectron spectroscopy</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0Eov-4cAcZ9YKQ3Dqx46xvVAsLldquVJVz5NiT1lVip57kAN-D74u7WxaJQyVLtuf95nmsR8jbgp8UXOhTx0PilSpk94Lsl1xyJkRdvtydldwjB4j3nCstlHhN9kSplaqE3ie_z4YRUhxgSt7SW9ODnXxggvphmENECBgTaw2Co7exdzSYEEeTJm97YN0cMh6D6f2vRyCZ8c5niVqT2hho8NnWwaYJ7wAmpCY4amMClu99T288u1yvPi_XK8zlYYzoJ8Aj8qozPcKbp_2Q_Fh9vVl-Zxfrb-fLswtmZakn1upaLqztLG-tFmDrmru2EqY1lTXWqAXnYHQLtSqqYiGgLura8U5apaRwwohD8nHrO6b4MANOzeDR5rlMgDhjU0pZ5Tfyyujxf-h9nFP--YbSpVIFrzL1aUvZFBETdM2Y_GDSz6bgzWNYzRd-db0Ja5Xh90-WczuA26F_08nAuy2Q0O7Uf2ln_cNzejO6TvwBOPun4Q</recordid><startdate>20201014</startdate><enddate>20201014</enddate><creator>Yola, Mehmet Lütfi</creator><creator>Atar, Necip</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7424-3425</orcidid></search><sort><creationdate>20201014</creationdate><title>Amperometric galectin-3 immunosensor-based gold nanoparticle-functionalized graphitic carbon nitride nanosheets and core-shell Ti-MOF@COFs composites</title><author>Yola, Mehmet Lütfi ; Atar, Necip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-b9748ccfc0bc93ec770db53aba5caca6800ea9be7615183e7177d0f4c6643d3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amplification</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Biomarkers</topic><topic>Biosensing Techniques</topic><topic>Carbon nitride</topic><topic>Electrical measurement</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Electrochemical Techniques</topic><topic>Electron microscopy</topic><topic>Fibrosis</topic><topic>Fourier transforms</topic><topic>Galactosides</topic><topic>Galectin 3</topic><topic>Galectins</topic><topic>Gold</topic><topic>Graphite</topic><topic>Immunoassay</topic><topic>Immunosensors</topic><topic>Limit of Detection</topic><topic>Linearity</topic><topic>Metal Nanoparticles</topic><topic>Metal-Organic Frameworks</topic><topic>Microscopy</topic><topic>Nanoparticles</topic><topic>Nanosheets</topic><topic>Nitrogen Compounds</topic><topic>Photoelectrons</topic><topic>Proteins</topic><topic>Selectivity</topic><topic>Spectrum analysis</topic><topic>Titanium</topic><topic>X ray photoelectron spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yola, Mehmet Lütfi</creatorcontrib><creatorcontrib>Atar, Necip</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yola, Mehmet Lütfi</au><au>Atar, Necip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amperometric galectin-3 immunosensor-based gold nanoparticle-functionalized graphitic carbon nitride nanosheets and core-shell Ti-MOF@COFs composites</atitle><jtitle>Nanoscale</jtitle><addtitle>Nanoscale</addtitle><date>2020-10-14</date><risdate>2020</risdate><volume>12</volume><issue>38</issue><spage>19824</spage><epage>19832</epage><pages>19824-19832</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><abstract>Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk. In this study, a novel sensitive amperometric method for antigen GL-3 detection is developed based on gold nanoparticle-functionalized graphitic carbon nitride nanosheets (g-C
3
N
4
@Au NPs) as the sensor platform and Ti-based metal organic framework (Ti-MOF, NH
2
-MIL-125)@covalent organic frameworks (COFs) composite for the signal amplification. The Ti-MOF@COF composite not only facilitates the penetration of antibody proteins into pore channels, but also the highly stable antigen-antibody interactions. The prepared sensor platform and signal amplification material are characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) method, X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The amperometric technique is utilized to achieve antigen GL-3 detection in plasma samples. The immunosensor demonstrates a wide linearity range (0.0001-20.0 ng mL
−1
) and a low detection limit (0.025 pg mL
−1
). Finally, the prepared immunosensor shows high stability and selectivity under optimum conditions.
Antigen galectin-3 (GL-3), a member of β-galactoside proteins indicates cardiac fibrosis and is a significant biomarker for monitoring heart failure risk and death risk.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>32966539</pmid><doi>10.1039/d0nr05614f</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7424-3425</orcidid></addata></record> |
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| issn | 2040-3364 2040-3372 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals |
| subjects | Amplification Antibodies Antigens Biomarkers Biosensing Techniques Carbon nitride Electrical measurement Electrochemical impedance spectroscopy Electrochemical Techniques Electron microscopy Fibrosis Fourier transforms Galactosides Galectin 3 Galectins Gold Graphite Immunoassay Immunosensors Limit of Detection Linearity Metal Nanoparticles Metal-Organic Frameworks Microscopy Nanoparticles Nanosheets Nitrogen Compounds Photoelectrons Proteins Selectivity Spectrum analysis Titanium X ray photoelectron spectroscopy |
| title | Amperometric galectin-3 immunosensor-based gold nanoparticle-functionalized graphitic carbon nitride nanosheets and core-shell Ti-MOF@COFs composites |
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