A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides

Hydrophilic Fe 3 O 4 @PDA@UIO-66-NH 2 is employed to consecutively enrich urinary exosomes and exosomal phosphopeptides through the strong bond between Zr and phosphate groups. The recovery of exosomes reached 95% and 707 phosphopeptides were identified from 255 phosphoproteins via LC-MS/MS. This wo...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2020-11, Vol.56 (9), p.13999-142
Hauptverfasser:	Zhang, Ning, Sun, Nianrong, Deng, Chunhui
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Liquid Ethical standards Exosomes - chemistry Functional analysis Humans Hydrophilicity Hydrophobic and Hydrophilic Interactions Iron oxides Magnetic Phenomena MCF-7 Cells Metal-Organic Frameworks - chemistry Particle Size Phosphopeptides - chemistry Surface Properties Tandem Mass Spectrometry Zirconium
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Beschreibung
Zusammenfassung:	Hydrophilic Fe 3 O 4 @PDA@UIO-66-NH 2 is employed to consecutively enrich urinary exosomes and exosomal phosphopeptides through the strong bond between Zr and phosphate groups. The recovery of exosomes reached 95% and 707 phosphopeptides were identified from 255 phosphoproteins via LC-MS/MS. This work will make a great contribution to further functional analysis of exosomes. Fe 3 O 4 @PDA@UIO-66-NH 2 is a biocompatible material that can be applied to enrich exosomes and phosphopeptides from urinary exosomes consecutively.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/d0cc06147f