Detection of locus-specific -methyladenosine modification based on Ag-assisted ligation and supersandwich signal amplification

Emerging evidence reveals that the epitranscriptomic mark N 6 -methyladenosine (m 6 A) plays vital roles in organisms, including gene regulation and disease progression. However, developing sensitive methods to detect m 6 A modification, especially the identification of m 6 A marks at the single-sit...

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Veröffentlicht in:Analyst (London) 2021-02, Vol.146 (4), p.1355-136
Hauptverfasser: Wang, Yanxia, Zheng, Ji, Duan, Chengjie, Jiao, Jin, Gong, Youjing, Shi, Hai, Xiang, Yang
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container_title Analyst (London)
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Zheng, Ji
Duan, Chengjie
Jiao, Jin
Gong, Youjing
Shi, Hai
Xiang, Yang
description Emerging evidence reveals that the epitranscriptomic mark N 6 -methyladenosine (m 6 A) plays vital roles in organisms, including gene regulation and disease progression. However, developing sensitive methods to detect m 6 A modification, especially the identification of m 6 A marks at the single-site level, remains a challenge. Therefore, based on target-specific triggered signal amplification, we developed a highly sensitive electrochemical method to detect site-specific m 6 A modifications in DNA. In this work, the m 6 A site in DNA can restrict the ligation assisted by Ag + , and this restriction effect can activate the subsequent strand displacement reaction and hybridization chain reaction (HCR), thus achieving signal amplification from the m 6 A site, and finally realizing high sensitivity analysis of m 6 A methylation. Benefiting from the high specificity of base pairs and the extremely weak binding affinity between Ag + and m 6 A, the proposed method was used for not only detecting the target DNA with a putative m 6 A site, but also identifying m 6 A marks at the single-site level in DNA. In addition, this study does not rely on antibodies and radiolabeling, so it has the advantage of cost-effectiveness. Therefore, we believe that the proposed strategy may provide a new perspective for methylation research, which can be used to test more clinical samples in further research. Emerging evidence reveals that the epitranscriptomic mark N 6 -methyladenosine (m 6 A) plays vital roles in organisms, including gene regulation and disease progression.
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title Detection of locus-specific -methyladenosine modification based on Ag-assisted ligation and supersandwich signal amplification
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