Detection of locus-specific -methyladenosine modification based on Ag-assisted ligation and supersandwich signal amplification
Emerging evidence reveals that the epitranscriptomic mark N 6 -methyladenosine (m 6 A) plays vital roles in organisms, including gene regulation and disease progression. However, developing sensitive methods to detect m 6 A modification, especially the identification of m 6 A marks at the single-sit...
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creator | Wang, Yanxia Zheng, Ji Duan, Chengjie Jiao, Jin Gong, Youjing Shi, Hai Xiang, Yang |
description | Emerging evidence reveals that the epitranscriptomic mark
N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression. However, developing sensitive methods to detect m
6
A modification, especially the identification of m
6
A marks at the single-site level, remains a challenge. Therefore, based on target-specific triggered signal amplification, we developed a highly sensitive electrochemical method to detect site-specific m
6
A modifications in DNA. In this work, the m
6
A site in DNA can restrict the ligation assisted by Ag
+
, and this restriction effect can activate the subsequent strand displacement reaction and hybridization chain reaction (HCR), thus achieving signal amplification from the m
6
A site, and finally realizing high sensitivity analysis of m
6
A methylation. Benefiting from the high specificity of base pairs and the extremely weak binding affinity between Ag
+
and m
6
A, the proposed method was used for not only detecting the target DNA with a putative m
6
A site, but also identifying m
6
A marks at the single-site level in DNA. In addition, this study does not rely on antibodies and radiolabeling, so it has the advantage of cost-effectiveness. Therefore, we believe that the proposed strategy may provide a new perspective for methylation research, which can be used to test more clinical samples in further research.
Emerging evidence reveals that the epitranscriptomic mark
N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression. |
doi_str_mv | 10.1039/d0an02214d |
format | Article |
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N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression. However, developing sensitive methods to detect m
6
A modification, especially the identification of m
6
A marks at the single-site level, remains a challenge. Therefore, based on target-specific triggered signal amplification, we developed a highly sensitive electrochemical method to detect site-specific m
6
A modifications in DNA. In this work, the m
6
A site in DNA can restrict the ligation assisted by Ag
+
, and this restriction effect can activate the subsequent strand displacement reaction and hybridization chain reaction (HCR), thus achieving signal amplification from the m
6
A site, and finally realizing high sensitivity analysis of m
6
A methylation. Benefiting from the high specificity of base pairs and the extremely weak binding affinity between Ag
+
and m
6
A, the proposed method was used for not only detecting the target DNA with a putative m
6
A site, but also identifying m
6
A marks at the single-site level in DNA. In addition, this study does not rely on antibodies and radiolabeling, so it has the advantage of cost-effectiveness. Therefore, we believe that the proposed strategy may provide a new perspective for methylation research, which can be used to test more clinical samples in further research.
Emerging evidence reveals that the epitranscriptomic mark
N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d0an02214d</identifier><ispartof>Analyst (London), 2021-02, Vol.146 (4), p.1355-136</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2832,27924,27925</link.rule.ids></links><search><creatorcontrib>Wang, Yanxia</creatorcontrib><creatorcontrib>Zheng, Ji</creatorcontrib><creatorcontrib>Duan, Chengjie</creatorcontrib><creatorcontrib>Jiao, Jin</creatorcontrib><creatorcontrib>Gong, Youjing</creatorcontrib><creatorcontrib>Shi, Hai</creatorcontrib><creatorcontrib>Xiang, Yang</creatorcontrib><title>Detection of locus-specific -methyladenosine modification based on Ag-assisted ligation and supersandwich signal amplification</title><title>Analyst (London)</title><description>Emerging evidence reveals that the epitranscriptomic mark
N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression. However, developing sensitive methods to detect m
6
A modification, especially the identification of m
6
A marks at the single-site level, remains a challenge. Therefore, based on target-specific triggered signal amplification, we developed a highly sensitive electrochemical method to detect site-specific m
6
A modifications in DNA. In this work, the m
6
A site in DNA can restrict the ligation assisted by Ag
+
, and this restriction effect can activate the subsequent strand displacement reaction and hybridization chain reaction (HCR), thus achieving signal amplification from the m
6
A site, and finally realizing high sensitivity analysis of m
6
A methylation. Benefiting from the high specificity of base pairs and the extremely weak binding affinity between Ag
+
and m
6
A, the proposed method was used for not only detecting the target DNA with a putative m
6
A site, but also identifying m
6
A marks at the single-site level in DNA. In addition, this study does not rely on antibodies and radiolabeling, so it has the advantage of cost-effectiveness. Therefore, we believe that the proposed strategy may provide a new perspective for methylation research, which can be used to test more clinical samples in further research.
Emerging evidence reveals that the epitranscriptomic mark
N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression.</description><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFj81qwzAQhEVpoe7PpfeAXkDNyj-hPZakoQ-Qe9hIa2eLLBmvQ8ilzx6nCemxp5nhGwZGqRcLrxaK96kHjJDntvQ3KrPFrDRVlb_dqgwACpPPqvJePYh8j9FCBZn6WdBAbuAUdap1SG4nRjpyXLPTpqVhewjoKSbhSLpN_gTwt79BIa9H89EYFGEZxhi4OVOMXsuuo15Gt2e31cJNxKCx7cJ15End1RiEni_6qCbLz9X8y_Ti1l3PLfaH9d-p4j9-BMnpUug</recordid><startdate>20210222</startdate><enddate>20210222</enddate><creator>Wang, Yanxia</creator><creator>Zheng, Ji</creator><creator>Duan, Chengjie</creator><creator>Jiao, Jin</creator><creator>Gong, Youjing</creator><creator>Shi, Hai</creator><creator>Xiang, Yang</creator><scope/></search><sort><creationdate>20210222</creationdate><title>Detection of locus-specific -methyladenosine modification based on Ag-assisted ligation and supersandwich signal amplification</title><author>Wang, Yanxia ; Zheng, Ji ; Duan, Chengjie ; Jiao, Jin ; Gong, Youjing ; Shi, Hai ; Xiang, Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-rsc_primary_d0an02214d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><creationdate>2021</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yanxia</creatorcontrib><creatorcontrib>Zheng, Ji</creatorcontrib><creatorcontrib>Duan, Chengjie</creatorcontrib><creatorcontrib>Jiao, Jin</creatorcontrib><creatorcontrib>Gong, Youjing</creatorcontrib><creatorcontrib>Shi, Hai</creatorcontrib><creatorcontrib>Xiang, Yang</creatorcontrib><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yanxia</au><au>Zheng, Ji</au><au>Duan, Chengjie</au><au>Jiao, Jin</au><au>Gong, Youjing</au><au>Shi, Hai</au><au>Xiang, Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of locus-specific -methyladenosine modification based on Ag-assisted ligation and supersandwich signal amplification</atitle><jtitle>Analyst (London)</jtitle><date>2021-02-22</date><risdate>2021</risdate><volume>146</volume><issue>4</issue><spage>1355</spage><epage>136</epage><pages>1355-136</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Emerging evidence reveals that the epitranscriptomic mark
N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression. However, developing sensitive methods to detect m
6
A modification, especially the identification of m
6
A marks at the single-site level, remains a challenge. Therefore, based on target-specific triggered signal amplification, we developed a highly sensitive electrochemical method to detect site-specific m
6
A modifications in DNA. In this work, the m
6
A site in DNA can restrict the ligation assisted by Ag
+
, and this restriction effect can activate the subsequent strand displacement reaction and hybridization chain reaction (HCR), thus achieving signal amplification from the m
6
A site, and finally realizing high sensitivity analysis of m
6
A methylation. Benefiting from the high specificity of base pairs and the extremely weak binding affinity between Ag
+
and m
6
A, the proposed method was used for not only detecting the target DNA with a putative m
6
A site, but also identifying m
6
A marks at the single-site level in DNA. In addition, this study does not rely on antibodies and radiolabeling, so it has the advantage of cost-effectiveness. Therefore, we believe that the proposed strategy may provide a new perspective for methylation research, which can be used to test more clinical samples in further research.
Emerging evidence reveals that the epitranscriptomic mark
N
6
-methyladenosine (m
6
A) plays vital roles in organisms, including gene regulation and disease progression.</abstract><doi>10.1039/d0an02214d</doi><tpages>6</tpages></addata></record> |
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source | Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
title | Detection of locus-specific -methyladenosine modification based on Ag-assisted ligation and supersandwich signal amplification |
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