High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots
In vivo cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for in vitro and in vivo tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots...
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| Veröffentlicht in: | RSC advances 2019-04, Vol.9 (19), p.1966-1975 |
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| container_end_page | 1975 |
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| container_issue | 19 |
| container_start_page | 1966 |
| container_title | RSC advances |
| container_volume | 9 |
| creator | Tang, Shiyi Guo, Yixiao Yang, Yidian Li, Yao Gao, Yanhong Zhang, Chunfu Xiong, Liqin |
| description | In vivo
cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for
in vitro
and
in vivo
tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots showed cellular uptake of approximately 98% within a short incubation time of 2 h, and these polymer dots maintained a cell labeling rate over 97% after 2 d. Moreover, a CCK-8 assay suggested that these polymer dots increased Ana-1 cell viabilities up to 110% at concentrations from 5 to 50 μg mL
−1
. Furthermore, the
in vivo
real time imaging of labelled Ana-1 cells in the alveolus of lung and lymph nodes were clearly detected by probe-based confocal laser endomicroscopy (pCLE). This study demonstrates a unique approach using polymer dots for real-time high resolution tracking of macrophage cells in deep organs and the lymphatic system.
Fluorescent polymer dots for tracking macrophage cells in deep organs using probe-based confocal laser endomicroscopy (pCLE). |
| doi_str_mv | 10.1039/c9ra00954j |
| format | Article |
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cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for
in vitro
and
in vivo
tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots showed cellular uptake of approximately 98% within a short incubation time of 2 h, and these polymer dots maintained a cell labeling rate over 97% after 2 d. Moreover, a CCK-8 assay suggested that these polymer dots increased Ana-1 cell viabilities up to 110% at concentrations from 5 to 50 μg mL
−1
. Furthermore, the
in vivo
real time imaging of labelled Ana-1 cells in the alveolus of lung and lymph nodes were clearly detected by probe-based confocal laser endomicroscopy (pCLE). This study demonstrates a unique approach using polymer dots for real-time high resolution tracking of macrophage cells in deep organs and the lymphatic system.
Fluorescent polymer dots for tracking macrophage cells in deep organs using probe-based confocal laser endomicroscopy (pCLE).</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c9ra00954j</identifier><identifier>PMID: 35515275</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Cell adhesion & migration ; Chemical synthesis ; Chemistry ; Flow cytometry ; Fluorescence ; High resolution ; Organs ; Polymers ; Real time ; Tracking</subject><ispartof>RSC advances, 2019-04, Vol.9 (19), p.1966-1975</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2019</rights><rights>This journal is © The Royal Society of Chemistry 2019 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-90a91d040990a26963ea40fdf8b352e4201dcaf5a4d039b0a8d86ce1fc1d167e3</citedby><cites>FETCH-LOGICAL-c428t-90a91d040990a26963ea40fdf8b352e4201dcaf5a4d039b0a8d86ce1fc1d167e3</cites><orcidid>0000-0001-8611-9691 ; 0000-0001-6993-415X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062640/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062640/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35515275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Shiyi</creatorcontrib><creatorcontrib>Guo, Yixiao</creatorcontrib><creatorcontrib>Yang, Yidian</creatorcontrib><creatorcontrib>Li, Yao</creatorcontrib><creatorcontrib>Gao, Yanhong</creatorcontrib><creatorcontrib>Zhang, Chunfu</creatorcontrib><creatorcontrib>Xiong, Liqin</creatorcontrib><title>High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>In vivo
cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for
in vitro
and
in vivo
tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots showed cellular uptake of approximately 98% within a short incubation time of 2 h, and these polymer dots maintained a cell labeling rate over 97% after 2 d. Moreover, a CCK-8 assay suggested that these polymer dots increased Ana-1 cell viabilities up to 110% at concentrations from 5 to 50 μg mL
−1
. Furthermore, the
in vivo
real time imaging of labelled Ana-1 cells in the alveolus of lung and lymph nodes were clearly detected by probe-based confocal laser endomicroscopy (pCLE). This study demonstrates a unique approach using polymer dots for real-time high resolution tracking of macrophage cells in deep organs and the lymphatic system.
Fluorescent polymer dots for tracking macrophage cells in deep organs using probe-based confocal laser endomicroscopy (pCLE).</description><subject>Cell adhesion & migration</subject><subject>Chemical synthesis</subject><subject>Chemistry</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>High resolution</subject><subject>Organs</subject><subject>Polymers</subject><subject>Real time</subject><subject>Tracking</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpdkt1rHCEUxaUkNCHJS98bhLyEwqZXR93xJRCWtmkIBEr7LK46s25ndKozgfz3cbPp5sMXL9yfh3PuFaFPBC4IVPKrkUkDSM7WH9AhBSZmFITce1UfoJOc11CO4IQK8hEdVJwTTuf8EPlr365wcjl20-hjwGPS5q8PLY4N7rVJcVjp1mHjui5jH7B1bsAxtTpkrIPF3UNfiNGbjKe8edd0Uyx6xoURD7G0XcI2jvkY7Te6y-7k-T5Cf75_-724nt3e_fi5uLqdGUbrcSZBS2KBgSwVFVJUTjNobFMvK04do0Cs0Q3XzJb4S9C1rYVxpDHEEjF31RG63OoO07J3duMj6U4Nyfc6PaiovXrbCX6l2nivJAgqGBSB82eBFP9NLo-q93mTXwcXp6yoEARqSuu6oGfv0HWcUijxFKXAq7kUnBXqy5Yq08w5uWZnhoDaLFEt5K-rpyXeFPj0tf0d-n9lBfi8BVI2u-7LL6geAQP2oyw</recordid><startdate>20190409</startdate><enddate>20190409</enddate><creator>Tang, Shiyi</creator><creator>Guo, Yixiao</creator><creator>Yang, Yidian</creator><creator>Li, Yao</creator><creator>Gao, Yanhong</creator><creator>Zhang, Chunfu</creator><creator>Xiong, Liqin</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8611-9691</orcidid><orcidid>https://orcid.org/0000-0001-6993-415X</orcidid></search><sort><creationdate>20190409</creationdate><title>High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots</title><author>Tang, Shiyi ; Guo, Yixiao ; Yang, Yidian ; Li, Yao ; Gao, Yanhong ; Zhang, Chunfu ; Xiong, Liqin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-90a91d040990a26963ea40fdf8b352e4201dcaf5a4d039b0a8d86ce1fc1d167e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Cell adhesion & migration</topic><topic>Chemical synthesis</topic><topic>Chemistry</topic><topic>Flow cytometry</topic><topic>Fluorescence</topic><topic>High resolution</topic><topic>Organs</topic><topic>Polymers</topic><topic>Real time</topic><topic>Tracking</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Shiyi</creatorcontrib><creatorcontrib>Guo, Yixiao</creatorcontrib><creatorcontrib>Yang, Yidian</creatorcontrib><creatorcontrib>Li, Yao</creatorcontrib><creatorcontrib>Gao, Yanhong</creatorcontrib><creatorcontrib>Zhang, Chunfu</creatorcontrib><creatorcontrib>Xiong, Liqin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Shiyi</au><au>Guo, Yixiao</au><au>Yang, Yidian</au><au>Li, Yao</au><au>Gao, Yanhong</au><au>Zhang, Chunfu</au><au>Xiong, Liqin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2019-04-09</date><risdate>2019</risdate><volume>9</volume><issue>19</issue><spage>1966</spage><epage>1975</epage><pages>1966-1975</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>In vivo
cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for
in vitro
and
in vivo
tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots showed cellular uptake of approximately 98% within a short incubation time of 2 h, and these polymer dots maintained a cell labeling rate over 97% after 2 d. Moreover, a CCK-8 assay suggested that these polymer dots increased Ana-1 cell viabilities up to 110% at concentrations from 5 to 50 μg mL
−1
. Furthermore, the
in vivo
real time imaging of labelled Ana-1 cells in the alveolus of lung and lymph nodes were clearly detected by probe-based confocal laser endomicroscopy (pCLE). This study demonstrates a unique approach using polymer dots for real-time high resolution tracking of macrophage cells in deep organs and the lymphatic system.
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| language | eng |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cell adhesion & migration Chemical synthesis Chemistry Flow cytometry Fluorescence High resolution Organs Polymers Real time Tracking |
| title | High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots |
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