A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor for detection of cardiac troponin I
In this study, a wavelength-resolved electrochemiluminescence resonance energy transfer (ECL-RET) ratiometric immunosensor from Au nanoparticle functionalized graphite-like carbon nitride nanosheets (Au-g-C 3 N 4 ) to Au nanoclusters (Au NCs) has been constructed for the first time. At a working vol...
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| Veröffentlicht in: | Analyst (London) 2019-11, Vol.144 (22), p.6554-656 |
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| creator | Zhu, Liping Ye, Jing Yan, Mengxia Zhu, Qiuju Yang, Xiurong |
| description | In this study, a wavelength-resolved electrochemiluminescence resonance energy transfer (ECL-RET) ratiometric immunosensor from Au nanoparticle functionalized graphite-like carbon nitride nanosheets (Au-g-C
3
N
4
) to Au nanoclusters (Au NCs) has been constructed for the first time. At a working voltage of 0 to −1.2 V, Au-g-C
3
N
4
showed a strong cathodic ECL emission with a peak at 460 nm, which overlapped well with the absorption spectra of Au NCs thus stimulating the fluorescence emission of Au NCs at 610 nm. Moreover, within this voltage range, the Au NCs showed no ECL signal; therefore, they would not interfere with the detection of the system. We used cardiac troponin I (cTnI) as an analytical model to construct a sandwich immunosensor based on the ECL-RET ratiometric strategy. By measuring the responses of the ECL
460 nm
/FL
610 nm
ratio at different cTnI concentrations, the sensitive detection of cTnI with a wide range of 50 fg mL
−1
to 50 ng mL
−1
and a low detection limit of 9.73 fg mL
−1
can be achieved. This work enriches the wavelength-resolved ECL-RET system and provides an innovative reference for the development of more efficient and sensitive ECL-RET ratiometry.
A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor from Au-g-C
3
N
4
to Au NCs has been constructed for the first time. |
| doi_str_mv | 10.1039/c9an01445d |
| format | Article |
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3
N
4
) to Au nanoclusters (Au NCs) has been constructed for the first time. At a working voltage of 0 to −1.2 V, Au-g-C
3
N
4
showed a strong cathodic ECL emission with a peak at 460 nm, which overlapped well with the absorption spectra of Au NCs thus stimulating the fluorescence emission of Au NCs at 610 nm. Moreover, within this voltage range, the Au NCs showed no ECL signal; therefore, they would not interfere with the detection of the system. We used cardiac troponin I (cTnI) as an analytical model to construct a sandwich immunosensor based on the ECL-RET ratiometric strategy. By measuring the responses of the ECL
460 nm
/FL
610 nm
ratio at different cTnI concentrations, the sensitive detection of cTnI with a wide range of 50 fg mL
−1
to 50 ng mL
−1
and a low detection limit of 9.73 fg mL
−1
can be achieved. This work enriches the wavelength-resolved ECL-RET system and provides an innovative reference for the development of more efficient and sensitive ECL-RET ratiometry.
A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor from Au-g-C
3
N
4
to Au NCs has been constructed for the first time.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c9an01445d</identifier><identifier>PMID: 31576385</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Absorption spectra ; Antibodies, Immobilized - immunology ; Biosensing Techniques - methods ; Carbon nitride ; Electric potential ; Electrochemical Techniques - methods ; Electrochemiluminescence ; Emission ; Energy Transfer ; Fluorescence ; Gold ; Gold - chemistry ; Graphite - chemistry ; Humans ; Immunoassay - methods ; Immunosensors ; Limit of Detection ; Luminescent Measurements - methods ; Metal Nanoparticles - chemistry ; Nanoparticles ; Nitrogen Compounds - chemistry ; Reproducibility of Results ; Resonance ; Sandwich structures ; Troponin I - blood ; Troponin I - immunology ; Voltage</subject><ispartof>Analyst (London), 2019-11, Vol.144 (22), p.6554-656</ispartof><rights>Copyright Royal Society of Chemistry 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-851e865a0fc631308b005ed87d386ba5fcfdd65f60fc1b6c1f8df46ce73d5a933</citedby><cites>FETCH-LOGICAL-c337t-851e865a0fc631308b005ed87d386ba5fcfdd65f60fc1b6c1f8df46ce73d5a933</cites><orcidid>0000-0003-0021-5135</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,2832,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31576385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Liping</creatorcontrib><creatorcontrib>Ye, Jing</creatorcontrib><creatorcontrib>Yan, Mengxia</creatorcontrib><creatorcontrib>Zhu, Qiuju</creatorcontrib><creatorcontrib>Yang, Xiurong</creatorcontrib><title>A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor for detection of cardiac troponin I</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>In this study, a wavelength-resolved electrochemiluminescence resonance energy transfer (ECL-RET) ratiometric immunosensor from Au nanoparticle functionalized graphite-like carbon nitride nanosheets (Au-g-C
3
N
4
) to Au nanoclusters (Au NCs) has been constructed for the first time. At a working voltage of 0 to −1.2 V, Au-g-C
3
N
4
showed a strong cathodic ECL emission with a peak at 460 nm, which overlapped well with the absorption spectra of Au NCs thus stimulating the fluorescence emission of Au NCs at 610 nm. Moreover, within this voltage range, the Au NCs showed no ECL signal; therefore, they would not interfere with the detection of the system. We used cardiac troponin I (cTnI) as an analytical model to construct a sandwich immunosensor based on the ECL-RET ratiometric strategy. By measuring the responses of the ECL
460 nm
/FL
610 nm
ratio at different cTnI concentrations, the sensitive detection of cTnI with a wide range of 50 fg mL
−1
to 50 ng mL
−1
and a low detection limit of 9.73 fg mL
−1
can be achieved. This work enriches the wavelength-resolved ECL-RET system and provides an innovative reference for the development of more efficient and sensitive ECL-RET ratiometry.
A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor from Au-g-C
3
N
4
to Au NCs has been constructed for the first time.</description><subject>Absorption spectra</subject><subject>Antibodies, Immobilized - immunology</subject><subject>Biosensing Techniques - methods</subject><subject>Carbon nitride</subject><subject>Electric potential</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrochemiluminescence</subject><subject>Emission</subject><subject>Energy Transfer</subject><subject>Fluorescence</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Graphite - chemistry</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunosensors</subject><subject>Limit of Detection</subject><subject>Luminescent Measurements - methods</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanoparticles</subject><subject>Nitrogen Compounds - chemistry</subject><subject>Reproducibility of Results</subject><subject>Resonance</subject><subject>Sandwich structures</subject><subject>Troponin I - blood</subject><subject>Troponin I - immunology</subject><subject>Voltage</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1PxCAQhonR6Lp68a4h8WJMqrAUSo-b9TMxetFzw8KgmBZWaDX-AP-3rOtH4mEyw_DwZoYXoT1KTihh9amulSe0LLlZQyPKRFlwPpHraEQIYcVE8HILbaf0nI-UcLKJthjllWCSj9DHFL-pV2jBP_ZPRYQU2lcwODd0H4N-gs61Q-c8JA1eA14SXi0r8BAf33EflU8WIo6qd6GDPjqNXdcNPiTwKURscxjos6ALHgeLtYrGKZ2fhkXwzuPrHbRhVZtg9zuP0cPF-f3sqri5u7yeTW8KzVjVF5JTkIIrYrVglBE5J4SDkZVhUswVt9oaI7gVGaBzoamVxpZCQ8UMVzVjY3S00l3E8DJA6pvO5cXaVnkIQ2omLH9RTSdZfIwO_6HPYYg-T5cpSismy2pJHa8oHUNKEWyziK5T8b2hpFma08zq6e2XOWcZPviWHOYdmF_0x40M7K-AmPTv7Z-77BNr4pco</recordid><startdate>20191104</startdate><enddate>20191104</enddate><creator>Zhu, Liping</creator><creator>Ye, Jing</creator><creator>Yan, Mengxia</creator><creator>Zhu, Qiuju</creator><creator>Yang, Xiurong</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0021-5135</orcidid></search><sort><creationdate>20191104</creationdate><title>A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor for detection of cardiac troponin I</title><author>Zhu, Liping ; Ye, Jing ; Yan, Mengxia ; Zhu, Qiuju ; Yang, Xiurong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-851e865a0fc631308b005ed87d386ba5fcfdd65f60fc1b6c1f8df46ce73d5a933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Absorption spectra</topic><topic>Antibodies, Immobilized - immunology</topic><topic>Biosensing Techniques - methods</topic><topic>Carbon nitride</topic><topic>Electric potential</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrochemiluminescence</topic><topic>Emission</topic><topic>Energy Transfer</topic><topic>Fluorescence</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Graphite - chemistry</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunosensors</topic><topic>Limit of Detection</topic><topic>Luminescent Measurements - methods</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanoparticles</topic><topic>Nitrogen Compounds - chemistry</topic><topic>Reproducibility of Results</topic><topic>Resonance</topic><topic>Sandwich structures</topic><topic>Troponin I - blood</topic><topic>Troponin I - immunology</topic><topic>Voltage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Liping</creatorcontrib><creatorcontrib>Ye, Jing</creatorcontrib><creatorcontrib>Yan, Mengxia</creatorcontrib><creatorcontrib>Zhu, Qiuju</creatorcontrib><creatorcontrib>Yang, Xiurong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Liping</au><au>Ye, Jing</au><au>Yan, Mengxia</au><au>Zhu, Qiuju</au><au>Yang, Xiurong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor for detection of cardiac troponin I</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2019-11-04</date><risdate>2019</risdate><volume>144</volume><issue>22</issue><spage>6554</spage><epage>656</epage><pages>6554-656</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>In this study, a wavelength-resolved electrochemiluminescence resonance energy transfer (ECL-RET) ratiometric immunosensor from Au nanoparticle functionalized graphite-like carbon nitride nanosheets (Au-g-C
3
N
4
) to Au nanoclusters (Au NCs) has been constructed for the first time. At a working voltage of 0 to −1.2 V, Au-g-C
3
N
4
showed a strong cathodic ECL emission with a peak at 460 nm, which overlapped well with the absorption spectra of Au NCs thus stimulating the fluorescence emission of Au NCs at 610 nm. Moreover, within this voltage range, the Au NCs showed no ECL signal; therefore, they would not interfere with the detection of the system. We used cardiac troponin I (cTnI) as an analytical model to construct a sandwich immunosensor based on the ECL-RET ratiometric strategy. By measuring the responses of the ECL
460 nm
/FL
610 nm
ratio at different cTnI concentrations, the sensitive detection of cTnI with a wide range of 50 fg mL
−1
to 50 ng mL
−1
and a low detection limit of 9.73 fg mL
−1
can be achieved. This work enriches the wavelength-resolved ECL-RET system and provides an innovative reference for the development of more efficient and sensitive ECL-RET ratiometry.
A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor from Au-g-C
3
N
4
to Au NCs has been constructed for the first time.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>31576385</pmid><doi>10.1039/c9an01445d</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0021-5135</orcidid></addata></record> |
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| ispartof | Analyst (London), 2019-11, Vol.144 (22), p.6554-656 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_rsc_primary_c9an01445d |
| source | MEDLINE; Royal Society Of Chemistry Journals; Royal Society of Chemistry Journals Archive (1841-2007); Alma/SFX Local Collection |
| subjects | Absorption spectra Antibodies, Immobilized - immunology Biosensing Techniques - methods Carbon nitride Electric potential Electrochemical Techniques - methods Electrochemiluminescence Emission Energy Transfer Fluorescence Gold Gold - chemistry Graphite - chemistry Humans Immunoassay - methods Immunosensors Limit of Detection Luminescent Measurements - methods Metal Nanoparticles - chemistry Nanoparticles Nitrogen Compounds - chemistry Reproducibility of Results Resonance Sandwich structures Troponin I - blood Troponin I - immunology Voltage |
| title | A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor for detection of cardiac troponin I |
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