A 2′,2′-disulfide-bridged dinucleotide conformationally locks RNA hairpinsElectronic supplementary information (ESI) available: Synthesis of cytidine, adenosine and guanosine phosphoramidite 1a-c and modified sequences, NMR spectra of intermediate and phosphoramidite 1a-c, analytical data of 2′-O-disulfide-bridged RNA sequences, Tm data and CD spectra of ONs, gel electrophoresis with SVPDE, fluorescence emission spectra with labelled ONs. See DOI: 10.1039/c8ob00328a

A 2′,2′-disulfide-bridged dinucleotide conformationally locks RNA hairpinsElectronic supplementary information (ESI) available: Synthesis of cytidine, adenosine and guanosine phosphoramidite 1a-c and modified sequences, NMR spectra of intermediate and phosphoramidite 1a-c, analytical data of 2′-O-disulfide-bridged RNA sequences, Tm data and CD spectra of ONs, gel electrophoresis with SVPDE, fluorescence emission spectra with labelled ONs. See DOI: 10.1039/c8ob00328a

The synthesis and the impact of a disulfide bridge between 2′- O -positions of two adjacent nucleotides in an RNA duplex and in the loop of RNA hairpins are reported. The incorporation of this 2′,2′-disulfide (S-S) bridge enabled thermal and enzymatic stabilization of the hairpin depending on its po...

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Hauptverfasser: Gauthier, Florian, Beltran, Frédéric, Biscans, Annabelle, Debart, Françoise, Dupouy, Christelle, Vasseur, Jean-Jacques
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Sprache:eng
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