Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy
Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultan...
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| Veröffentlicht in: | Nanoscale 2018-10, Vol.1 (4), p.19125-1913 |
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| creator | Spicer, Graham L. C Almassalha, Luay Martinez, Ignacio A Ellis, Ronald Chandler, John E Gladstein, Scott Zhang, Di Nguyen, The-Quyen Feder, Seth Subramanian, Hariharan de la Rica, Roberto Thompson, Sebastian A Backman, Vadim |
| description | Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultaneously image and perform spectral analysis of GNPs. Here, we present a study utilizing a recently introduced hyperspectral imaging technique, live-cell PWS, for the imaging, tracking, and spectral analysis of GNPs in live cancer cells. Using principal components analysis, the extracellular or intracellular localization of the GNPs can be determined without the use of exogenous labels. This technique uses wide-field white light, assuring minimal toxicity and suitable signal-to-noise ratio for spectral and temporal resolution of backscattered signal from GNPs and local cellular structures. The application of live-cell PWS introduced here could make a great impact in nanomedicine and nanotechnology by giving new insights into GNP internalization and intracellular trafficking.
Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. |
| doi_str_mv | 10.1039/c8nr07481j |
| format | Article |
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Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c8nr07481j</identifier><identifier>PMID: 30298892</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Absorption spectra ; Backscattering ; Biological properties ; Cancer ; Cellular structure ; Electron beams ; Electron bombardment ; Gold ; Gold - chemistry ; Gold - pharmacology ; HeLa Cells ; Humans ; Hyperspectral imaging ; Localization ; Materials Testing ; Metal Nanoparticles - chemistry ; Nanoparticles ; Nanotechnology ; Neoplasms - diagnostic imaging ; Neoplasms - metabolism ; Principal components analysis ; Temporal resolution ; Toxicity ; White light</subject><ispartof>Nanoscale, 2018-10, Vol.1 (4), p.19125-1913</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-6e8867032e5a4bec2e290370262b99c98662b280d86aa7ba01a7e7f4399b076b3</citedby><cites>FETCH-LOGICAL-c374t-6e8867032e5a4bec2e290370262b99c98662b280d86aa7ba01a7e7f4399b076b3</cites><orcidid>0000-0002-5750-1469 ; 0000-0002-0196-1124</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30298892$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spicer, Graham L. C</creatorcontrib><creatorcontrib>Almassalha, Luay</creatorcontrib><creatorcontrib>Martinez, Ignacio A</creatorcontrib><creatorcontrib>Ellis, Ronald</creatorcontrib><creatorcontrib>Chandler, John E</creatorcontrib><creatorcontrib>Gladstein, Scott</creatorcontrib><creatorcontrib>Zhang, Di</creatorcontrib><creatorcontrib>Nguyen, The-Quyen</creatorcontrib><creatorcontrib>Feder, Seth</creatorcontrib><creatorcontrib>Subramanian, Hariharan</creatorcontrib><creatorcontrib>de la Rica, Roberto</creatorcontrib><creatorcontrib>Thompson, Sebastian A</creatorcontrib><creatorcontrib>Backman, Vadim</creatorcontrib><title>Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultaneously image and perform spectral analysis of GNPs. Here, we present a study utilizing a recently introduced hyperspectral imaging technique, live-cell PWS, for the imaging, tracking, and spectral analysis of GNPs in live cancer cells. Using principal components analysis, the extracellular or intracellular localization of the GNPs can be determined without the use of exogenous labels. This technique uses wide-field white light, assuring minimal toxicity and suitable signal-to-noise ratio for spectral and temporal resolution of backscattered signal from GNPs and local cellular structures. The application of live-cell PWS introduced here could make a great impact in nanomedicine and nanotechnology by giving new insights into GNP internalization and intracellular trafficking.
Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples.</description><subject>Absorption spectra</subject><subject>Backscattering</subject><subject>Biological properties</subject><subject>Cancer</subject><subject>Cellular structure</subject><subject>Electron beams</subject><subject>Electron bombardment</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Gold - pharmacology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Hyperspectral imaging</subject><subject>Localization</subject><subject>Materials Testing</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Neoplasms - diagnostic imaging</subject><subject>Neoplasms - metabolism</subject><subject>Principal components analysis</subject><subject>Temporal resolution</subject><subject>Toxicity</subject><subject>White light</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1LAzEQxYMoVqsX70rAm7CaTbb5OErxk6IgehOWbDorKWmyJrtC_evdurWe5sH8eDO8h9BJTi5zwtSVkT4SUch8sYMOKClIxpigu1vNixE6TGlBCFeMs300YoQqKRU9QO8zXYHDdQTALhjt7LdubfA41NhrHxodW2scJGw9dvYLsNHeQMQGnEu4S9Z_4NSAaWNIJjTW4KU1g14dob1auwTHmzlGb7c3r9P7bPZ89zC9nmWGiaLNOEjJBWEUJrqowFCgijBBKKeVUkZJ3gsqyVxyrUWlSa4FiLpgSlVE8IqN0fng28Tw2UFqy0Xoou9PljSnOZ9MpJI9dTFQ6_dShLpsol3quCpzUq6DLKfy6eU3yMcePttYdtUS5lv0L7keOB2AmMx2-98E-wF1fngn</recordid><startdate>20181018</startdate><enddate>20181018</enddate><creator>Spicer, Graham L. 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C ; Almassalha, Luay ; Martinez, Ignacio A ; Ellis, Ronald ; Chandler, John E ; Gladstein, Scott ; Zhang, Di ; Nguyen, The-Quyen ; Feder, Seth ; Subramanian, Hariharan ; de la Rica, Roberto ; Thompson, Sebastian A ; Backman, Vadim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-6e8867032e5a4bec2e290370262b99c98662b280d86aa7ba01a7e7f4399b076b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Absorption spectra</topic><topic>Backscattering</topic><topic>Biological properties</topic><topic>Cancer</topic><topic>Cellular structure</topic><topic>Electron beams</topic><topic>Electron bombardment</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Gold - pharmacology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Hyperspectral imaging</topic><topic>Localization</topic><topic>Materials Testing</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Neoplasms - diagnostic imaging</topic><topic>Neoplasms - metabolism</topic><topic>Principal components analysis</topic><topic>Temporal resolution</topic><topic>Toxicity</topic><topic>White light</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spicer, Graham L. C</creatorcontrib><creatorcontrib>Almassalha, Luay</creatorcontrib><creatorcontrib>Martinez, Ignacio A</creatorcontrib><creatorcontrib>Ellis, Ronald</creatorcontrib><creatorcontrib>Chandler, John E</creatorcontrib><creatorcontrib>Gladstein, Scott</creatorcontrib><creatorcontrib>Zhang, Di</creatorcontrib><creatorcontrib>Nguyen, The-Quyen</creatorcontrib><creatorcontrib>Feder, Seth</creatorcontrib><creatorcontrib>Subramanian, Hariharan</creatorcontrib><creatorcontrib>de la Rica, Roberto</creatorcontrib><creatorcontrib>Thompson, Sebastian A</creatorcontrib><creatorcontrib>Backman, Vadim</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spicer, Graham L. C</au><au>Almassalha, Luay</au><au>Martinez, Ignacio A</au><au>Ellis, Ronald</au><au>Chandler, John E</au><au>Gladstein, Scott</au><au>Zhang, Di</au><au>Nguyen, The-Quyen</au><au>Feder, Seth</au><au>Subramanian, Hariharan</au><au>de la Rica, Roberto</au><au>Thompson, Sebastian A</au><au>Backman, Vadim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy</atitle><jtitle>Nanoscale</jtitle><addtitle>Nanoscale</addtitle><date>2018-10-18</date><risdate>2018</risdate><volume>1</volume><issue>4</issue><spage>19125</spage><epage>1913</epage><pages>19125-1913</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><abstract>Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultaneously image and perform spectral analysis of GNPs. Here, we present a study utilizing a recently introduced hyperspectral imaging technique, live-cell PWS, for the imaging, tracking, and spectral analysis of GNPs in live cancer cells. Using principal components analysis, the extracellular or intracellular localization of the GNPs can be determined without the use of exogenous labels. This technique uses wide-field white light, assuring minimal toxicity and suitable signal-to-noise ratio for spectral and temporal resolution of backscattered signal from GNPs and local cellular structures. The application of live-cell PWS introduced here could make a great impact in nanomedicine and nanotechnology by giving new insights into GNP internalization and intracellular trafficking.
Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>30298892</pmid><doi>10.1039/c8nr07481j</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-5750-1469</orcidid><orcidid>https://orcid.org/0000-0002-0196-1124</orcidid></addata></record> |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
| subjects | Absorption spectra Backscattering Biological properties Cancer Cellular structure Electron beams Electron bombardment Gold Gold - chemistry Gold - pharmacology HeLa Cells Humans Hyperspectral imaging Localization Materials Testing Metal Nanoparticles - chemistry Nanoparticles Nanotechnology Neoplasms - diagnostic imaging Neoplasms - metabolism Principal components analysis Temporal resolution Toxicity White light |
| title | Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy |
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