A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain
Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm 2 ,...
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| Veröffentlicht in: | Lab on a chip 2019-04, Vol.19 (8), p.1332-1343 |
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| creator | van den Brink, Floris T. G Phisonkunkasem, Thas Asthana, Ashish Bomer, Johan G van den Maagdenberg, Arn M. J. M Tolner, Else A Odijk, Mathieu |
| description | Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm
2
, and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push-pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61%, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for
in vivo
recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.
Measuring neurotransmitters in the brain of living animals is a challenging task, especially when detailed information at high temporal resolution is required. |
| doi_str_mv | 10.1039/c8lc01137k |
| format | Article |
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2
, and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push-pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61%, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for
in vivo
recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.
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2
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in vivo
recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.
Measuring neurotransmitters in the brain of living animals is a challenging task, especially when detailed information at high temporal resolution is required.</description><subject>Animals</subject><subject>Brain</subject><subject>Equipment Design</subject><subject>Feasibility studies</subject><subject>Lab-On-A-Chip Devices</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neurotransmitter Agents - metabolism</subject><subject>Neurotransmitters</subject><subject>Optical Phenomena</subject><subject>Real time</subject><subject>Risk management</subject><subject>Sampling</subject><subject>Temporal resolution</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1LHTEUhkOx1K9uurcEuhFhNJlkPrKUi7bSC27a9ZCPk97oTDImE6T-eqPX3oKrc-A8vLw8B6EvlJxTwsSF7kdNKGXd_Qd0QHnHKkJ7sbfbRbePDlO6I4Q2vO0_oX1G-la0HTlAm0s8Oe_kkqN7AoPnnDbVnMexmiHanFzweI5BAbYhYguPVQIdvMFJTvPo_B8cLPaQY1ii9GlyywIxYefxsgE8hZwAqyidP0YfrRwTfH6bR-j39dWv1Y9qffv9ZnW5rjRv6FIxyw03NWXcWuCdoNBb3kstiFY1U-XIOO2VkNJqMFIp2TLViAaAUNMaw47Q6Ta3tH7IkJZhcknDOEoPpc1QU0FZw4qugn57h96FHH1pN9Q1KarapmsKdbaldAwpRbDDHN0k49-BkuHF_7Dq16tX_z8L_PUtMqsJzA79J7wAJ1sgJr27_n8gewaGc4wc</recordid><startdate>20190409</startdate><enddate>20190409</enddate><creator>van den Brink, Floris T. 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2
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in vivo
recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Brain Equipment Design Feasibility studies Lab-On-A-Chip Devices Male Mice Mice, Inbred C57BL Neurotransmitter Agents - metabolism Neurotransmitters Optical Phenomena Real time Risk management Sampling Temporal resolution |
| title | A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain |
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