A multifunctional toolkit for target-directed cancer therapyElectronic supplementary information (ESI) available. See DOI: 10.1039/c8cc08823c

Here we present 2shRNA, a shRNA-based nanobinder, which can simultaneously attack two therapeutic targets involved in drug resistance pathways and can additionally bind accessory molecules such as cell targeting peptides or fluorophores. We create 2shRNAs designed to specifically kill HER2+ breast cancer cells in the absence of a transfecting agent. A novel tumor-targeted RNA toolkit encoding multiple functions in a single structure.