Bacterial growth sensing in microgels using pH-dependent fluorescence emission
We report the development of a platform for sensing bacterial growth by observing pH change via a change in fluorescence. While the platform allows for the rapid detection of bacteria it is sensitive enough to detect even ∼10 4 CFU of bacteria. The platform can differentiate between resistant and no...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2018-02, Vol.54 (13), p.1643-1646 |
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| container_title | Chemical communications (Cambridge, England) |
| container_volume | 54 |
| creator | Chandra, Anil Singh, Neetu |
| description | We report the development of a platform for sensing bacterial growth by observing pH change
via
a change in fluorescence. While the platform allows for the rapid detection of bacteria it is sensitive enough to detect even ∼10
4
CFU of bacteria. The platform can differentiate between resistant and non-resistant strains of
E. coli
in ∼4 to 6 hours, which is better than currently used clinical methods.
Fast sensing of bacterial growth and antibiotic resistance by observing pH variation in microgels encapsulating bacteria and carbon dots. |
| doi_str_mv | 10.1039/c7cc08678d |
| format | Article |
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via
a change in fluorescence. While the platform allows for the rapid detection of bacteria it is sensitive enough to detect even ∼10
4
CFU of bacteria. The platform can differentiate between resistant and non-resistant strains of
E. coli
in ∼4 to 6 hours, which is better than currently used clinical methods.
Fast sensing of bacterial growth and antibiotic resistance by observing pH variation in microgels encapsulating bacteria and carbon dots.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c7cc08678d</identifier><identifier>PMID: 29376166</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Alginates - chemistry ; Ampicillin - pharmacology ; Anti-Bacterial Agents - pharmacology ; Bacteria ; Bacterial Load - methods ; Carbon - chemistry ; E coli ; Escherichia coli - growth & development ; Fluorescence ; Hydrogels - chemistry ; Hydrogen-Ion Concentration ; Microbial Sensitivity Tests ; Microgels ; Nanostructures - chemistry ; Particle Size ; Staphylococcus aureus - growth & development</subject><ispartof>Chemical communications (Cambridge, England), 2018-02, Vol.54 (13), p.1643-1646</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-fdd1e4f9114a177919768e8a9b076a0ca422defb05c19a6cc22c8fdeb746dff3</citedby><cites>FETCH-LOGICAL-c337t-fdd1e4f9114a177919768e8a9b076a0ca422defb05c19a6cc22c8fdeb746dff3</cites><orcidid>0000-0003-4553-931X ; 0000-0002-7880-4880</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29376166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chandra, Anil</creatorcontrib><creatorcontrib>Singh, Neetu</creatorcontrib><title>Bacterial growth sensing in microgels using pH-dependent fluorescence emission</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>We report the development of a platform for sensing bacterial growth by observing pH change
via
a change in fluorescence. While the platform allows for the rapid detection of bacteria it is sensitive enough to detect even ∼10
4
CFU of bacteria. The platform can differentiate between resistant and non-resistant strains of
E. coli
in ∼4 to 6 hours, which is better than currently used clinical methods.
Fast sensing of bacterial growth and antibiotic resistance by observing pH variation in microgels encapsulating bacteria and carbon dots.</description><subject>Alginates - chemistry</subject><subject>Ampicillin - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteria</subject><subject>Bacterial Load - methods</subject><subject>Carbon - chemistry</subject><subject>E coli</subject><subject>Escherichia coli - growth & development</subject><subject>Fluorescence</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Microbial Sensitivity Tests</subject><subject>Microgels</subject><subject>Nanostructures - chemistry</subject><subject>Particle Size</subject><subject>Staphylococcus aureus - growth & development</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0U1PwyAYB3BiNG5OL941TbwYkyqUlpej1peZLHrZwVvD4GF26eiENsZvL3tRE7lA4JfngT8InRJ8TTCVN5prjQXjwuyhIaEsT4tcvO2v14VMOc2LAToKYYHjIIU4RINMUs4IY0P0cqd0B75WTTL37Wf3ngRwoXbzpHbJsta-nUMTkn6ztRqnBlbgDLgusU3fegganIYElnUIdeuO0YFVTYCT3TxC08eHaTlOJ69Pz-XtJNWU8i61xhDIrSQkV4RzSSRnAoSSM8yZwlrlWWbAznChiVRM6yzTwhqY8ZwZa-kIXW7Lrnz70UPoqthfQ9MoB20fKiIlxVgWOI_04h9dtL138XJVFgnLikyIqK62Kj44BA-2Wvl6qfxXRXC1DrkqeVluQr6P-HxXsp8twfzSn1QjONsCH_Tv6d8v0W-TkYGy</recordid><startdate>20180208</startdate><enddate>20180208</enddate><creator>Chandra, Anil</creator><creator>Singh, Neetu</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4553-931X</orcidid><orcidid>https://orcid.org/0000-0002-7880-4880</orcidid></search><sort><creationdate>20180208</creationdate><title>Bacterial growth sensing in microgels using pH-dependent fluorescence emission</title><author>Chandra, Anil ; Singh, Neetu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-fdd1e4f9114a177919768e8a9b076a0ca422defb05c19a6cc22c8fdeb746dff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alginates - chemistry</topic><topic>Ampicillin - pharmacology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteria</topic><topic>Bacterial Load - methods</topic><topic>Carbon - chemistry</topic><topic>E coli</topic><topic>Escherichia coli - growth & development</topic><topic>Fluorescence</topic><topic>Hydrogels - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>Microbial Sensitivity Tests</topic><topic>Microgels</topic><topic>Nanostructures - chemistry</topic><topic>Particle Size</topic><topic>Staphylococcus aureus - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chandra, Anil</creatorcontrib><creatorcontrib>Singh, Neetu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chandra, Anil</au><au>Singh, Neetu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial growth sensing in microgels using pH-dependent fluorescence emission</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2018-02-08</date><risdate>2018</risdate><volume>54</volume><issue>13</issue><spage>1643</spage><epage>1646</epage><pages>1643-1646</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>We report the development of a platform for sensing bacterial growth by observing pH change
via
a change in fluorescence. While the platform allows for the rapid detection of bacteria it is sensitive enough to detect even ∼10
4
CFU of bacteria. The platform can differentiate between resistant and non-resistant strains of
E. coli
in ∼4 to 6 hours, which is better than currently used clinical methods.
Fast sensing of bacterial growth and antibiotic resistance by observing pH variation in microgels encapsulating bacteria and carbon dots.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29376166</pmid><doi>10.1039/c7cc08678d</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-4553-931X</orcidid><orcidid>https://orcid.org/0000-0002-7880-4880</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2018-02, Vol.54 (13), p.1643-1646 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_rsc_primary_c7cc08678d |
| source | MEDLINE; Alma/SFX Local Collection; Royal Society of Chemistry |
| subjects | Alginates - chemistry Ampicillin - pharmacology Anti-Bacterial Agents - pharmacology Bacteria Bacterial Load - methods Carbon - chemistry E coli Escherichia coli - growth & development Fluorescence Hydrogels - chemistry Hydrogen-Ion Concentration Microbial Sensitivity Tests Microgels Nanostructures - chemistry Particle Size Staphylococcus aureus - growth & development |
| title | Bacterial growth sensing in microgels using pH-dependent fluorescence emission |
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