New visible light excitable donor-acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovaniElectronic supplementary information (ESI) available: Spectroscopic characterization data, 1H and 13C NMR spectra, and absorption, excitation and fluorescence spectra of all of the compounds, 4a-d, 5, 6 and 8, in HEPES. Computed TD-DFT data and Cartesian coordinates, materials and methods. See DOI: 10.1039/c6tb03257e
In order to address the existing limitations of the commercially available fluorescent probe CMAC (7-amino-4-chloromethylcoumarin), a new series of highly fluorescent donor-acceptor 7-hydroxy-coumarin derivatives was prepared and these derivatives were used as vacuole specific fluorescent probes for...
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