A new H2S-specific near-infrared fluorescence-enhanced probe that can visualize the H2S level in colorectal cancer cells in miceElectronic supplementary information (ESI) available: Experimental details, photophysical data, some fluorescence imaging figures, average fluorescence intensity figures and in vivo imaging. See DOI: 10.1039/c6sc05646f

Near-infrared (NIR) fluorescence-based sensors capable of selective detection of H 2 S in vivo would be useful tools to understand the mechanisms of diseases. A new NIR fluorescence probe 1 was developed for the detection of endogenous H 2 S in colorectal cancer cells in mice. 1 displayed an 87-fold...

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Hauptverfasser: Zhang, Kun, Zhang, Jie, Xi, Zhen, Li, Lu-Yuan, Gu, Xiangxiang, Zhang, Qiang-Zhe, Yi, Long
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Sprache:eng
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Zusammenfassung:Near-infrared (NIR) fluorescence-based sensors capable of selective detection of H 2 S in vivo would be useful tools to understand the mechanisms of diseases. A new NIR fluorescence probe 1 was developed for the detection of endogenous H 2 S in colorectal cancer cells in mice. 1 displayed an 87-fold fluorescence enhancement at 796 nm (with excitation at 730 nm) when reacted with H 2 S in a buffer (pH 7.4). 1 was water-soluble, cell-membrane-permeable, had low cytotoxicity and high selectivity and sensitivity for H 2 S. The properties of 1 enable its use in monitoring endogenous H 2 S in living cells, tissues, and mice. The bioimaging results indicated that (1) d -Cys could induce endogenous H 2 S production in living cells and stimulate angiogenesis; (2) tail intravenous injection of 1 into mice generated strong fluorescence in the liver while intraperitoneal injection of d -Cys could further enhance fluorescence in the liver in vivo ; (3) importantly, endogenous H 2 S in colorectal cancer cells (HCT116, HT29) in vitro and in murine tumor models could be quickly and selectively detected by intratumoral injection of 1 . These results indicated that our new probe could serve as an efficient tool for the detection of cellular H 2 S in living animals and even for cancer diagnosis. A highly sensitive H 2 S-specific near-infrared fluorescence-enhanced probe was developed for real-time imaging of endogenous H 2 S in colorectal cancer cells (HCT116 and HT29) in mice.
ISSN:2041-6520
2041-6539
DOI:10.1039/c6sc05646f