A new H2S-specific near-infrared fluorescence-enhanced probe that can visualize the H2S level in colorectal cancer cells in miceElectronic supplementary information (ESI) available: Experimental details, photophysical data, some fluorescence imaging figures, average fluorescence intensity figures and in vivo imaging. See DOI: 10.1039/c6sc05646f
Near-infrared (NIR) fluorescence-based sensors capable of selective detection of H 2 S in vivo would be useful tools to understand the mechanisms of diseases. A new NIR fluorescence probe 1 was developed for the detection of endogenous H 2 S in colorectal cancer cells in mice. 1 displayed an 87-fold...
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Zusammenfassung: | Near-infrared (NIR) fluorescence-based sensors capable of selective detection of H
2
S
in vivo
would be useful tools to understand the mechanisms of diseases. A new NIR fluorescence probe
1
was developed for the detection of endogenous H
2
S in colorectal cancer cells in mice.
1
displayed an 87-fold fluorescence enhancement at 796 nm (with excitation at 730 nm) when reacted with H
2
S in a buffer (pH 7.4).
1
was water-soluble, cell-membrane-permeable, had low cytotoxicity and high selectivity and sensitivity for H
2
S. The properties of
1
enable its use in monitoring endogenous H
2
S in living cells, tissues, and mice. The bioimaging results indicated that (1)
d
-Cys could induce endogenous H
2
S production in living cells and stimulate angiogenesis; (2) tail intravenous injection of
1
into mice generated strong fluorescence in the liver while intraperitoneal injection of
d
-Cys could further enhance fluorescence in the liver
in vivo
; (3) importantly, endogenous H
2
S in colorectal cancer cells (HCT116, HT29)
in vitro
and in murine tumor models could be quickly and selectively detected by intratumoral injection of
1
. These results indicated that our new probe could serve as an efficient tool for the detection of cellular H
2
S in living animals and even for cancer diagnosis.
A highly sensitive H
2
S-specific near-infrared fluorescence-enhanced probe was developed for real-time imaging of endogenous H
2
S in colorectal cancer cells (HCT116 and HT29) in mice. |
---|---|
ISSN: | 2041-6520 2041-6539 |
DOI: | 10.1039/c6sc05646f |