Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics
Droplet-based microfluidics enables assays to be carried out at very high throughput (up to thousands of samples per second) and enables researchers to work with very limited material, such as primary cells, patient's biopsies or expensive reagents. An additional strength of the technology is t...
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| Veröffentlicht in: | Lab on a chip 2016-04, Vol.16 (8), p.1314-1331 |
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| creator | Shembekar, Nachiket Chaipan, Chawaree Utharala, Ramesh Merten, Christoph A |
| description | Droplet-based microfluidics enables assays to be carried out at very high throughput (up to thousands of samples per second) and enables researchers to work with very limited material, such as primary cells, patient's biopsies or expensive reagents. An additional strength of the technology is the possibility to perform large-scale genotypic or phenotypic screens at the single-cell level. Here we critically review the latest developments in antibody screening, drug discovery and highly multiplexed genomic applications such as targeted genetic workflows, single-cell RNAseq and single-cell ChIPseq. Starting with a comprehensive introduction for non-experts, we pinpoint current limitations, analyze how they might be overcome and give an outlook on exciting future applications.
This review focuses on developments in the field of droplet microfluidics in the context of drug discovery, genomics and transcriptomics. |
| doi_str_mv | 10.1039/c6lc00249h |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2016-04, Vol.16 (8), p.1314-1331 |
| issn | 1473-0197 1473-0189 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Antibodies Drug Discovery - instrumentation Drug Discovery - methods Drugs Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Genetics Humans Lab-On-A-Chip Devices Microfluidics Molecular Biology - instrumentation Molecular Biology - methods Multiplexing Reagents Screening Workflow |
| title | Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics |
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