Antithrombotic effect and mechanism of Rubus spp. Blackberry
The compounds of Rubus spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. The RSB extract was evaluated by activated partial thromboplastin time (APTT), thrombin time...
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| Veröffentlicht in: | Food & function 2017-05, Vol.8 (5), p.2-212 |
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| creator | Xie, Pingyao Zhang, Yong Wang, Xuebiao Wei, Jinfeng Kang, Wenyi |
| description | The compounds of
Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. The RSB extract was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) assays
in vitro
. Results indicated that RSB extract exhibited anticoagulant activity. In addition to compounds
1
and
6
, the other compounds also exhibited anticoagulant activity
in vitro
. Therefore, the
in vivo
antithrombosis effects of RSB extract were investigated by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of thromboxane B2 (TXB
2
), 6-keto prostaglandin F1α (6-keto-PGF1α), endothelial nitric oxide synthase (eNOS) and ET-1 (endothelin-1) were measured. Results suggested that RSB extract had inhibitory effects on thrombus formation, and its antithrombotic effects were associated with the regulation of vascular endothelium active substance, activation of blood flow and an anticoagulation effect.
The compounds of
Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. |
| doi_str_mv | 10.1039/c6fo01717g |
| format | Article |
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Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. The RSB extract was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) assays
in vitro
. Results indicated that RSB extract exhibited anticoagulant activity. In addition to compounds
1
and
6
, the other compounds also exhibited anticoagulant activity
in vitro
. Therefore, the
in vivo
antithrombosis effects of RSB extract were investigated by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of thromboxane B2 (TXB
2
), 6-keto prostaglandin F1α (6-keto-PGF1α), endothelial nitric oxide synthase (eNOS) and ET-1 (endothelin-1) were measured. Results suggested that RSB extract had inhibitory effects on thrombus formation, and its antithrombotic effects were associated with the regulation of vascular endothelium active substance, activation of blood flow and an anticoagulation effect.
The compounds of
Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/c6fo01717g</identifier><identifier>PMID: 28485425</identifier><language>eng</language><publisher>England</publisher><subject>6-Ketoprostaglandin F1 alpha - blood ; Animals ; Blood Coagulation - drug effects ; Female ; Fibrinolytic Agents - administration & dosage ; Fibrinolytic Agents - chemistry ; Humans ; Male ; Partial Thromboplastin Time ; Plant Extracts - administration & dosage ; Plant Extracts - chemistry ; Prothrombin Time ; Rabbits ; Rats ; Rats, Sprague-Dawley ; Rubus ; Rubus - chemistry ; Thrombin Time ; Thrombosis - blood ; Thrombosis - drug therapy ; Thromboxane B2 - blood</subject><ispartof>Food & function, 2017-05, Vol.8 (5), p.2-212</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-32d369686554acb1d02bdf906659064b698ca4f02993de6946e15faa3762885c3</citedby><cites>FETCH-LOGICAL-c409t-32d369686554acb1d02bdf906659064b698ca4f02993de6946e15faa3762885c3</cites><orcidid>0000-0002-1822-6249</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28485425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Pingyao</creatorcontrib><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Wang, Xuebiao</creatorcontrib><creatorcontrib>Wei, Jinfeng</creatorcontrib><creatorcontrib>Kang, Wenyi</creatorcontrib><title>Antithrombotic effect and mechanism of Rubus spp. Blackberry</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>The compounds of
Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. The RSB extract was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) assays
in vitro
. Results indicated that RSB extract exhibited anticoagulant activity. In addition to compounds
1
and
6
, the other compounds also exhibited anticoagulant activity
in vitro
. Therefore, the
in vivo
antithrombosis effects of RSB extract were investigated by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of thromboxane B2 (TXB
2
), 6-keto prostaglandin F1α (6-keto-PGF1α), endothelial nitric oxide synthase (eNOS) and ET-1 (endothelin-1) were measured. Results suggested that RSB extract had inhibitory effects on thrombus formation, and its antithrombotic effects were associated with the regulation of vascular endothelium active substance, activation of blood flow and an anticoagulation effect.
The compounds of
Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model.</description><subject>6-Ketoprostaglandin F1 alpha - blood</subject><subject>Animals</subject><subject>Blood Coagulation - drug effects</subject><subject>Female</subject><subject>Fibrinolytic Agents - administration & dosage</subject><subject>Fibrinolytic Agents - chemistry</subject><subject>Humans</subject><subject>Male</subject><subject>Partial Thromboplastin Time</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - chemistry</subject><subject>Prothrombin Time</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rubus</subject><subject>Rubus - chemistry</subject><subject>Thrombin Time</subject><subject>Thrombosis - blood</subject><subject>Thrombosis - drug therapy</subject><subject>Thromboxane B2 - blood</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLw0AQxhdRbKm9eFfiTYTUfWcXvNRgq1AoiIK3sNns2mhe7iaH_vem9uFN5zAz8P34GL4B4BzBCYJE3mpua4giFL0fgSGGFIecwbfj_U4lH4Cx9x-wLyKlkOIUDLCgglHMhuBuWrV5u3J1mdZtrgNjrdFtoKosKI1eqSr3ZVDb4LlLOx_4ppkE94XSn6lxbn0GTqwqvBnv5gi8zh5e4sdwsZw_xdNFqCmUbUhwRrjkgjNGlU5RBnGaWQk5Z32jKZdCK2ohlpJkhkvKDWJWKRJxLATTZASut76Nq78649ukzL02RaEqU3c-QRsbJhAn_6OiP0RiQqMevdmi2tXeO2OTxuWlcusEwWSTbRLz2fIn23kPX-58u7Q02QHdJ9kDF1vAeX1Qf5_T61d_6UmTWfINEtqG9g</recordid><startdate>20170524</startdate><enddate>20170524</enddate><creator>Xie, Pingyao</creator><creator>Zhang, Yong</creator><creator>Wang, Xuebiao</creator><creator>Wei, Jinfeng</creator><creator>Kang, Wenyi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><orcidid>https://orcid.org/0000-0002-1822-6249</orcidid></search><sort><creationdate>20170524</creationdate><title>Antithrombotic effect and mechanism of Rubus spp. Blackberry</title><author>Xie, Pingyao ; Zhang, Yong ; Wang, Xuebiao ; Wei, Jinfeng ; Kang, Wenyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-32d369686554acb1d02bdf906659064b698ca4f02993de6946e15faa3762885c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>6-Ketoprostaglandin F1 alpha - blood</topic><topic>Animals</topic><topic>Blood Coagulation - drug effects</topic><topic>Female</topic><topic>Fibrinolytic Agents - administration & dosage</topic><topic>Fibrinolytic Agents - chemistry</topic><topic>Humans</topic><topic>Male</topic><topic>Partial Thromboplastin Time</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - chemistry</topic><topic>Prothrombin Time</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rubus</topic><topic>Rubus - chemistry</topic><topic>Thrombin Time</topic><topic>Thrombosis - blood</topic><topic>Thrombosis - drug therapy</topic><topic>Thromboxane B2 - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Pingyao</creatorcontrib><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Wang, Xuebiao</creatorcontrib><creatorcontrib>Wei, Jinfeng</creatorcontrib><creatorcontrib>Kang, Wenyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Pingyao</au><au>Zhang, Yong</au><au>Wang, Xuebiao</au><au>Wei, Jinfeng</au><au>Kang, Wenyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antithrombotic effect and mechanism of Rubus spp. Blackberry</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2017-05-24</date><risdate>2017</risdate><volume>8</volume><issue>5</issue><spage>2</spage><epage>212</epage><pages>2-212</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>The compounds of
Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. The RSB extract was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) assays
in vitro
. Results indicated that RSB extract exhibited anticoagulant activity. In addition to compounds
1
and
6
, the other compounds also exhibited anticoagulant activity
in vitro
. Therefore, the
in vivo
antithrombosis effects of RSB extract were investigated by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of thromboxane B2 (TXB
2
), 6-keto prostaglandin F1α (6-keto-PGF1α), endothelial nitric oxide synthase (eNOS) and ET-1 (endothelin-1) were measured. Results suggested that RSB extract had inhibitory effects on thrombus formation, and its antithrombotic effects were associated with the regulation of vascular endothelium active substance, activation of blood flow and an anticoagulation effect.
The compounds of
Rubus
spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model.</abstract><cop>England</cop><pmid>28485425</pmid><doi>10.1039/c6fo01717g</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-1822-6249</orcidid></addata></record> |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
| subjects | 6-Ketoprostaglandin F1 alpha - blood Animals Blood Coagulation - drug effects Female Fibrinolytic Agents - administration & dosage Fibrinolytic Agents - chemistry Humans Male Partial Thromboplastin Time Plant Extracts - administration & dosage Plant Extracts - chemistry Prothrombin Time Rabbits Rats Rats, Sprague-Dawley Rubus Rubus - chemistry Thrombin Time Thrombosis - blood Thrombosis - drug therapy Thromboxane B2 - blood |
| title | Antithrombotic effect and mechanism of Rubus spp. Blackberry |
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