Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphateElectronic supplementary information (ESI) available. See DOI: 10.1039/c6cc05796a
Enzymatic synthesis and the reverse transcription of RNAs containing 2′- O -carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′- O -carbamoyl uridine triphosphate (U cm TP). U cm TP was incorporated correctly into long RNAs, and its fidelity during reve...
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| creator | Masaki, Yoshiaki Ito, Hyugo Oda, Yuki Yamazaki, Kazufumi Tago, Nobuhiro Ohno, Kentaro Ishii, Nozomi Tsunoda, Hirosuke Kanamori, Takashi Ohkubo, Akihiro Sekine, Mitsuo Seio, Kohji |
| description | Enzymatic synthesis and the reverse transcription of RNAs containing 2′-
O
-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′-
O
-carbamoyl uridine triphosphate (U
cm
TP). U
cm
TP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
Enzymatic synthesis and the reverse transcription of RNAs containing 2′-
O
-carbamoyl uridine were evaluated. |
| doi_str_mv | 10.1039/c6cc05796a |
| format | Article |
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O
-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′-
O
-carbamoyl uridine triphosphate (U
cm
TP). U
cm
TP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
Enzymatic synthesis and the reverse transcription of RNAs containing 2′-
O
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O
-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′-
O
-carbamoyl uridine triphosphate (U
cm
TP). U
cm
TP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
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O
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O
-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′-
O
-carbamoyl uridine triphosphate (U
cm
TP). U
cm
TP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
Enzymatic synthesis and the reverse transcription of RNAs containing 2′-
O
-carbamoyl uridine were evaluated.</abstract><doi>10.1039/c6cc05796a</doi><tpages>4</tpages></addata></record> |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| title | Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphateElectronic supplementary information (ESI) available. See DOI: 10.1039/c6cc05796a |
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