In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animalsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ay03066a
A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H 2 O 2 ) was characterised in freely-moving rats. The in situ sensitivity of the sensor was assessed by the direct delivery of H 2 O 2 to the local environment of the implanted sen...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1264 |
|---|---|
| container_issue | 8 |
| container_start_page | 1253 |
| container_title | |
| container_volume | 9 |
| creator | O'Riordan, Saidhbhe L Lowry, John P |
| description | A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats. The
in situ
sensitivity of the sensor was assessed by the direct delivery of H
2
O
2
to the local environment of the implanted sensor and by the chemical manipulation of the endogenous concentration of H
2
O
2
. Inhibitors of H
2
O
2
enzymatic degradation were utilised including sodium azide (SA) and mercaptosuccinate (MCS). SA and MCS primarily inhibit catalase and glutathione peroxidase (GPx) respectively and the application of each resulted in a significant increase in the H
2
O
2
signal. The selectivity of the sensor was verified by the absence of a change in the signal in response to the peripheral administration of ascorbic acid (AA) compared to controls. Evidence of a disparity between brain H
2
O
2
signalling in the freely-moving animal with respect to the anaesthetised subject was also observed. The enzymatic component of the paired H
2
O
2
sensor was found to be stable over a continuous monitoring period of 12 days, thereby demonstrating the suitability of this sensor for the long-term chronic detection of brain H
2
O
2
.
A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats. |
| doi_str_mv | 10.1039/c6ay03066a |
| format | Article |
| fullrecord | <record><control><sourceid>rsc</sourceid><recordid>TN_cdi_rsc_primary_c6ay03066a</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>c6ay03066a</sourcerecordid><originalsourceid>FETCH-rsc_primary_c6ay03066a3</originalsourceid><addsrcrecordid>eNqFUD1LA0EQXUTB-NHYC2OnxcU9DjecrUaSyiL2YW5vLlnZj2N2Pbz_6w9xQ0QLQYv5YN6b94YR4qKU01JW9a1WOMpKKoUHYlLO7uqiVrP68LtX8licxPgqpaorVU7Ex9LDYIYAeouMOhGbiMkED6EDBI0JLUYqmpxaaEyI5GNg6HIwoS2ScQRkSScOekvOaLTggjcpsPGbnUzDaDxsx5bDhjz0xOHdtAR52DGRHQsXhh0XvXFo43yv5o2G-Nb3lhz5hDzmhWzr9uddz1fLG8ABjcXG0hRWRPD4vLyH3784E0dd1qXzr3oqLp_mLw-LgqNe95xNeVz_0Kv_8au_8HXfdtUntwaBfA</addsrcrecordid><sourcetype>Enrichment Source</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animalsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ay03066a</title><source>Royal Society Of Chemistry Journals 2008-</source><creator>O'Riordan, Saidhbhe L ; Lowry, John P</creator><creatorcontrib>O'Riordan, Saidhbhe L ; Lowry, John P</creatorcontrib><description>A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats. The
in situ
sensitivity of the sensor was assessed by the direct delivery of H
2
O
2
to the local environment of the implanted sensor and by the chemical manipulation of the endogenous concentration of H
2
O
2
. Inhibitors of H
2
O
2
enzymatic degradation were utilised including sodium azide (SA) and mercaptosuccinate (MCS). SA and MCS primarily inhibit catalase and glutathione peroxidase (GPx) respectively and the application of each resulted in a significant increase in the H
2
O
2
signal. The selectivity of the sensor was verified by the absence of a change in the signal in response to the peripheral administration of ascorbic acid (AA) compared to controls. Evidence of a disparity between brain H
2
O
2
signalling in the freely-moving animal with respect to the anaesthetised subject was also observed. The enzymatic component of the paired H
2
O
2
sensor was found to be stable over a continuous monitoring period of 12 days, thereby demonstrating the suitability of this sensor for the long-term chronic detection of brain H
2
O
2
.
A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats.</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/c6ay03066a</identifier><language>eng</language><creationdate>2017-02</creationdate><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>O'Riordan, Saidhbhe L</creatorcontrib><creatorcontrib>Lowry, John P</creatorcontrib><title>In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animalsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ay03066a</title><description>A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats. The
in situ
sensitivity of the sensor was assessed by the direct delivery of H
2
O
2
to the local environment of the implanted sensor and by the chemical manipulation of the endogenous concentration of H
2
O
2
. Inhibitors of H
2
O
2
enzymatic degradation were utilised including sodium azide (SA) and mercaptosuccinate (MCS). SA and MCS primarily inhibit catalase and glutathione peroxidase (GPx) respectively and the application of each resulted in a significant increase in the H
2
O
2
signal. The selectivity of the sensor was verified by the absence of a change in the signal in response to the peripheral administration of ascorbic acid (AA) compared to controls. Evidence of a disparity between brain H
2
O
2
signalling in the freely-moving animal with respect to the anaesthetised subject was also observed. The enzymatic component of the paired H
2
O
2
sensor was found to be stable over a continuous monitoring period of 12 days, thereby demonstrating the suitability of this sensor for the long-term chronic detection of brain H
2
O
2
.
A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats.</description><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFUD1LA0EQXUTB-NHYC2OnxcU9DjecrUaSyiL2YW5vLlnZj2N2Pbz_6w9xQ0QLQYv5YN6b94YR4qKU01JW9a1WOMpKKoUHYlLO7uqiVrP68LtX8licxPgqpaorVU7Ex9LDYIYAeouMOhGbiMkED6EDBI0JLUYqmpxaaEyI5GNg6HIwoS2ScQRkSScOekvOaLTggjcpsPGbnUzDaDxsx5bDhjz0xOHdtAR52DGRHQsXhh0XvXFo43yv5o2G-Nb3lhz5hDzmhWzr9uddz1fLG8ABjcXG0hRWRPD4vLyH3784E0dd1qXzr3oqLp_mLw-LgqNe95xNeVz_0Kv_8au_8HXfdtUntwaBfA</recordid><startdate>20170223</startdate><enddate>20170223</enddate><creator>O'Riordan, Saidhbhe L</creator><creator>Lowry, John P</creator><scope/></search><sort><creationdate>20170223</creationdate><title>In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animalsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ay03066a</title><author>O'Riordan, Saidhbhe L ; Lowry, John P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-rsc_primary_c6ay03066a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><toplevel>online_resources</toplevel><creatorcontrib>O'Riordan, Saidhbhe L</creatorcontrib><creatorcontrib>Lowry, John P</creatorcontrib></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Riordan, Saidhbhe L</au><au>Lowry, John P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animalsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ay03066a</atitle><date>2017-02-23</date><risdate>2017</risdate><volume>9</volume><issue>8</issue><spage>1253</spage><epage>1264</epage><pages>1253-1264</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><abstract>A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats. The
in situ
sensitivity of the sensor was assessed by the direct delivery of H
2
O
2
to the local environment of the implanted sensor and by the chemical manipulation of the endogenous concentration of H
2
O
2
. Inhibitors of H
2
O
2
enzymatic degradation were utilised including sodium azide (SA) and mercaptosuccinate (MCS). SA and MCS primarily inhibit catalase and glutathione peroxidase (GPx) respectively and the application of each resulted in a significant increase in the H
2
O
2
signal. The selectivity of the sensor was verified by the absence of a change in the signal in response to the peripheral administration of ascorbic acid (AA) compared to controls. Evidence of a disparity between brain H
2
O
2
signalling in the freely-moving animal with respect to the anaesthetised subject was also observed. The enzymatic component of the paired H
2
O
2
sensor was found to be stable over a continuous monitoring period of 12 days, thereby demonstrating the suitability of this sensor for the long-term chronic detection of brain H
2
O
2
.
A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H
2
O
2
) was characterised in freely-moving rats.</abstract><doi>10.1039/c6ay03066a</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1759-9660 |
| ispartof | |
| issn | 1759-9660 1759-9679 |
| language | eng |
| recordid | cdi_rsc_primary_c6ay03066a |
| source | Royal Society Of Chemistry Journals 2008- |
| title | In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animalsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ay03066a |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T21%3A16%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-rsc&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vivo%20characterisation%20of%20a%20catalase-based%20biosensor%20for%20real-time%20electrochemical%20monitoring%20of%20brain%20hydrogen%20peroxide%20in%20freely-moving%20animalsElectronic%20supplementary%20information%20(ESI)%20available.%20See%20DOI:%2010.1039/c6ay03066a&rft.au=O'Riordan,%20Saidhbhe%20L&rft.date=2017-02-23&rft.volume=9&rft.issue=8&rft.spage=1253&rft.epage=1264&rft.pages=1253-1264&rft.issn=1759-9660&rft.eissn=1759-9679&rft_id=info:doi/10.1039/c6ay03066a&rft_dat=%3Crsc%3Ec6ay03066a%3C/rsc%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |