Development and validation of a fast and simple HPLC method for the simultaneous determination of aniline and its degradation products in wastewater
In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products, para -aminophenol, meta -aminophenol, ortho -amin...
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| Veröffentlicht in: | Analytical methods 2016-01, Vol.8 (3), p.5949-5956 |
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| creator | Boulahlib, Soumia Boudina, Ali Si-Ahmed, Kahina Bessekhouad, Yassine Trari, Mohamed |
| description | In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products,
para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed. The retention and selectivity of these pollutants were investigated by testing three different columns (C18, C8 and C4) and changing several experimental parameters such as the organic modifier nature and content, buffer concentration, pH value and column temperature. The best chromatographic results were obtained using the C4 column and a mobile phase composed of MeOH/acetate buffer (10 mM, pH 5) (60 : 40, v/v), with
λ
= 270 nm and a flow rate of 1 mL min
−1
. The influence of the temperature was also investigated in the range of 10-50 °C; the results indicated the dependence of the enthalpies of transfer of the analytes on the mobile phase and the nature of the stationary phase, and a good linear correlation of ln
k vs.
1/
T
was observed for all the tested columns. The linearity of the method ranged from 2.0 to 60 μg mL
−1
for all compounds, with correlation coefficients varying from 0.9987 to 0.9999. Recoveries of aniline and its degradation products in water ranged from 87.51% to 101.35% while the relative standard deviations varied between 0.31% and 1.62% (
n
= 6). The limits of quantification of the method ranged from 0.0778 to 0.2073 μg mL
−1
. The results show that the method we developed is fast, low cost and can meet the requirements for the determination of aniline and its degradation products in waste or natural waters.
In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products,
para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed. |
| doi_str_mv | 10.1039/c6ay01298a |
| format | Article |
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para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed. The retention and selectivity of these pollutants were investigated by testing three different columns (C18, C8 and C4) and changing several experimental parameters such as the organic modifier nature and content, buffer concentration, pH value and column temperature. The best chromatographic results were obtained using the C4 column and a mobile phase composed of MeOH/acetate buffer (10 mM, pH 5) (60 : 40, v/v), with
λ
= 270 nm and a flow rate of 1 mL min
−1
. The influence of the temperature was also investigated in the range of 10-50 °C; the results indicated the dependence of the enthalpies of transfer of the analytes on the mobile phase and the nature of the stationary phase, and a good linear correlation of ln
k vs.
1/
T
was observed for all the tested columns. The linearity of the method ranged from 2.0 to 60 μg mL
−1
for all compounds, with correlation coefficients varying from 0.9987 to 0.9999. Recoveries of aniline and its degradation products in water ranged from 87.51% to 101.35% while the relative standard deviations varied between 0.31% and 1.62% (
n
= 6). The limits of quantification of the method ranged from 0.0778 to 0.2073 μg mL
−1
. The results show that the method we developed is fast, low cost and can meet the requirements for the determination of aniline and its degradation products in waste or natural waters.
In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products,
para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed.</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/c6ay01298a</identifier><language>eng</language><subject>Acetates ; Aniline ; Buffers ; Degradation ; High performance liquid chromatography ; PDA ; Pollutants</subject><ispartof>Analytical methods, 2016-01, Vol.8 (3), p.5949-5956</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-b27c16cb54becb0b2f34f98a9827e9b590e43e8964ce23a0727de5b1aa414acf3</citedby><cites>FETCH-LOGICAL-c356t-b27c16cb54becb0b2f34f98a9827e9b590e43e8964ce23a0727de5b1aa414acf3</cites><orcidid>0000-0003-0285-6761</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Boulahlib, Soumia</creatorcontrib><creatorcontrib>Boudina, Ali</creatorcontrib><creatorcontrib>Si-Ahmed, Kahina</creatorcontrib><creatorcontrib>Bessekhouad, Yassine</creatorcontrib><creatorcontrib>Trari, Mohamed</creatorcontrib><title>Development and validation of a fast and simple HPLC method for the simultaneous determination of aniline and its degradation products in wastewater</title><title>Analytical methods</title><description>In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products,
para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed. The retention and selectivity of these pollutants were investigated by testing three different columns (C18, C8 and C4) and changing several experimental parameters such as the organic modifier nature and content, buffer concentration, pH value and column temperature. The best chromatographic results were obtained using the C4 column and a mobile phase composed of MeOH/acetate buffer (10 mM, pH 5) (60 : 40, v/v), with
λ
= 270 nm and a flow rate of 1 mL min
−1
. The influence of the temperature was also investigated in the range of 10-50 °C; the results indicated the dependence of the enthalpies of transfer of the analytes on the mobile phase and the nature of the stationary phase, and a good linear correlation of ln
k vs.
1/
T
was observed for all the tested columns. The linearity of the method ranged from 2.0 to 60 μg mL
−1
for all compounds, with correlation coefficients varying from 0.9987 to 0.9999. Recoveries of aniline and its degradation products in water ranged from 87.51% to 101.35% while the relative standard deviations varied between 0.31% and 1.62% (
n
= 6). The limits of quantification of the method ranged from 0.0778 to 0.2073 μg mL
−1
. The results show that the method we developed is fast, low cost and can meet the requirements for the determination of aniline and its degradation products in waste or natural waters.
In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products,
para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed.</description><subject>Acetates</subject><subject>Aniline</subject><subject>Buffers</subject><subject>Degradation</subject><subject>High performance liquid chromatography</subject><subject>PDA</subject><subject>Pollutants</subject><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkT1PwzAQhi0EEqWwsCN5REgBO06ceKzCR5EqwQADU-Q4F2qUL2ynVf8HPxi3qejK5JPvufe904vQJSW3lDBxp7jcEBqKVB6hCU1iEQieiOO_mpNTdGbtFyFcME4n6OceVlB3fQOtw7It8UrWupROdy3uKixxJe3YsLrpa8Dz10WGG3DLrsRVZ7BbwrY11E620A0Wl-DANLo9aLS61i3sRLTbAp9G7i1605WD8p-6xWvvBGvpp8_RSSVrCxf7d4reHx_esnmweHl6zmaLQLGYu6AIE0W5KuKoAFWQIqxYVPnTRRomIIpYEIgYpIJHCkImSRImJcQFlTKikVQVm6LrUdev8T2AdXmjrYK6Hk_Jaep9SJok7B8opWnqLbhHb0ZUmc5aA1XeG91Is8kpybcp5RmffexSmnn4aoSNVX_cIUX2C-uZkeU</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Boulahlib, Soumia</creator><creator>Boudina, Ali</creator><creator>Si-Ahmed, Kahina</creator><creator>Bessekhouad, Yassine</creator><creator>Trari, Mohamed</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7TV</scope><scope>C1K</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0003-0285-6761</orcidid></search><sort><creationdate>20160101</creationdate><title>Development and validation of a fast and simple HPLC method for the simultaneous determination of aniline and its degradation products in wastewater</title><author>Boulahlib, Soumia ; Boudina, Ali ; Si-Ahmed, Kahina ; Bessekhouad, Yassine ; Trari, Mohamed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-b27c16cb54becb0b2f34f98a9827e9b590e43e8964ce23a0727de5b1aa414acf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acetates</topic><topic>Aniline</topic><topic>Buffers</topic><topic>Degradation</topic><topic>High performance liquid chromatography</topic><topic>PDA</topic><topic>Pollutants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boulahlib, Soumia</creatorcontrib><creatorcontrib>Boudina, Ali</creatorcontrib><creatorcontrib>Si-Ahmed, Kahina</creatorcontrib><creatorcontrib>Bessekhouad, Yassine</creatorcontrib><creatorcontrib>Trari, Mohamed</creatorcontrib><collection>CrossRef</collection><collection>Pollution Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boulahlib, Soumia</au><au>Boudina, Ali</au><au>Si-Ahmed, Kahina</au><au>Bessekhouad, Yassine</au><au>Trari, Mohamed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of a fast and simple HPLC method for the simultaneous determination of aniline and its degradation products in wastewater</atitle><jtitle>Analytical methods</jtitle><date>2016-01-01</date><risdate>2016</risdate><volume>8</volume><issue>3</issue><spage>5949</spage><epage>5956</epage><pages>5949-5956</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><abstract>In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products,
para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed. The retention and selectivity of these pollutants were investigated by testing three different columns (C18, C8 and C4) and changing several experimental parameters such as the organic modifier nature and content, buffer concentration, pH value and column temperature. The best chromatographic results were obtained using the C4 column and a mobile phase composed of MeOH/acetate buffer (10 mM, pH 5) (60 : 40, v/v), with
λ
= 270 nm and a flow rate of 1 mL min
−1
. The influence of the temperature was also investigated in the range of 10-50 °C; the results indicated the dependence of the enthalpies of transfer of the analytes on the mobile phase and the nature of the stationary phase, and a good linear correlation of ln
k vs.
1/
T
was observed for all the tested columns. The linearity of the method ranged from 2.0 to 60 μg mL
−1
for all compounds, with correlation coefficients varying from 0.9987 to 0.9999. Recoveries of aniline and its degradation products in water ranged from 87.51% to 101.35% while the relative standard deviations varied between 0.31% and 1.62% (
n
= 6). The limits of quantification of the method ranged from 0.0778 to 0.2073 μg mL
−1
. The results show that the method we developed is fast, low cost and can meet the requirements for the determination of aniline and its degradation products in waste or natural waters.
In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products,
para
-aminophenol,
meta
-aminophenol,
ortho
-aminophenol and phenol, was developed.</abstract><doi>10.1039/c6ay01298a</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0285-6761</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1759-9660 |
| ispartof | Analytical methods, 2016-01, Vol.8 (3), p.5949-5956 |
| issn | 1759-9660 1759-9679 |
| language | eng |
| recordid | cdi_rsc_primary_c6ay01298a |
| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Acetates Aniline Buffers Degradation High performance liquid chromatography PDA Pollutants |
| title | Development and validation of a fast and simple HPLC method for the simultaneous determination of aniline and its degradation products in wastewater |
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