Grafted biomembranes containing membrane proteins - the case of the leucine transporterElectronic supplementary information (ESI) available: Materials, synthesis of NTA-PEG thiols, formation and characterization of the NTA-PEG SAM, optimization of the NTA-PEG content for minimal non-specific protein binding and LeuT binding on inactivated NTA-PEG surfaces. See DOI: 10.1039/c5sm01490e

Grafted biomembranes containing membrane proteins - the case of the leucine transporterElectronic supplementary information (ESI) available: Materials, synthesis of NTA-PEG thiols, formation and characterization of the NTA-PEG SAM, optimization of the NTA-PEG content for minimal non-specific protein binding and LeuT binding on inactivated NTA-PEG surfaces. See DOI: 10.1039/c5sm01490e

Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron refle...

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Hauptverfasser: Jagalski, Vivien, Barker, Robert D, Thygesen, Mikkel B, Gotfryd, Kamil, Krüger, Mie B, Shi, Lei, Maric, Selma, Bovet, Nicolas, Moulin, Martine, Haertlein, Michael, Günther Pomorski, Thomas, Loland, Claus J, Cárdenas, Marité
Format: Artikel
Sprache:eng
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