Development of antibacterial polyacrylonitrile membrane modified with a covalently immobilized lysozyme
A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared. First, the virginal PAN membranes were prepared via the classic immersion precipitation method. After modification with NaOH, HCl, ethylenediamine (EDA), the lysozyme was covalently immobilized...
Gespeichert in:
| Veröffentlicht in: | RSC advances 2015-01, Vol.5 (13), p.84432-84438 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 84438 |
|---|---|
| container_issue | 13 |
| container_start_page | 84432 |
| container_title | RSC advances |
| container_volume | 5 |
| creator | Liu, Wei Cai, Minhua He, Yuegui Wang, Shuai Zheng, Jinwang Xu, Xiaoping |
| description | A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared. First, the virginal PAN membranes were prepared
via
the classic immersion precipitation method. After modification with NaOH, HCl, ethylenediamine (EDA), the lysozyme was covalently immobilized onto the surface of the PAN membranes by glutaraldehyde. The chemical compositions of virginal and modified membranes were characterized by Fourier transform infrared spectroscopy (FT-IR) and Energy Dispersion X-ray (EDX). The morphology and performance of the immobilized membranes were characterized by Scanning Electronic Microscopy (SEM), filtration performance measurements, the amount of bonded lysozyme, lysozyme activity measurement and flow cytometry method. The antibacterial tests confirmed that the immobilized lysozyme membrane displayed an excellent antibacterial performance against
Staphylococcus aureus
(
S. aureus
).
A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared. |
| doi_str_mv | 10.1039/c5ra14867g |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_rsc_p</sourceid><recordid>TN_cdi_rsc_primary_c5ra14867g</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1727687293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c345t-d248b379015f4f393842cb0f528f493360e180cfc25d1bfcea3fe7d5a678e73b3</originalsourceid><addsrcrecordid>eNqNkc1LAzEQxYMoWGov3oV4E2E1H5tk91iqVqEgiJ6XbDapkWyzJtvK9q83WlFP4lxm4P14M7wB4BijC4xoealYkDgvuFjugRFBOc8I4uX-r_kQTGJ8Qak4w4TjEVhe6Y12vmv1qofeQLnqbS1Vr4OVDnbeDVKFwfmV7YN1Gra6rYNcpcE31ljdwDfbP0MJld9Il0zcAG3b-to6u02qG6LfDq0-AgdGuqgnX30Mnm6uH2e32eJ-fjebLjJFc9ZnDcmLmooSYWZyQ0ta5ETVyDBSmLyklCONC6SMIqzBtVFaUqNFwyQXhRa0pmNwtvPtgn9d69hXrY1KO5du9utYYcEJKhMt_oESwQtB0toxON-hKvgYgzZVF2wrw1BhVH1kX83Yw_Qz-3mCT3ZwiOqb-_lN0k__0quuMfQdqzWOKw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1727687293</pqid></control><display><type>article</type><title>Development of antibacterial polyacrylonitrile membrane modified with a covalently immobilized lysozyme</title><source>Royal Society Of Chemistry Journals 2008-</source><creator>Liu, Wei ; Cai, Minhua ; He, Yuegui ; Wang, Shuai ; Zheng, Jinwang ; Xu, Xiaoping</creator><creatorcontrib>Liu, Wei ; Cai, Minhua ; He, Yuegui ; Wang, Shuai ; Zheng, Jinwang ; Xu, Xiaoping</creatorcontrib><description>A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared. First, the virginal PAN membranes were prepared
via
the classic immersion precipitation method. After modification with NaOH, HCl, ethylenediamine (EDA), the lysozyme was covalently immobilized onto the surface of the PAN membranes by glutaraldehyde. The chemical compositions of virginal and modified membranes were characterized by Fourier transform infrared spectroscopy (FT-IR) and Energy Dispersion X-ray (EDX). The morphology and performance of the immobilized membranes were characterized by Scanning Electronic Microscopy (SEM), filtration performance measurements, the amount of bonded lysozyme, lysozyme activity measurement and flow cytometry method. The antibacterial tests confirmed that the immobilized lysozyme membrane displayed an excellent antibacterial performance against
Staphylococcus aureus
(
S. aureus
).
A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c5ra14867g</identifier><language>eng</language><subject>Antiinfectives and antibacterials ; Covalence ; Dispersions ; Electronics ; Flow cytometry ; Lysozyme ; Membranes ; Polyacrylonitriles ; Staphylococcus</subject><ispartof>RSC advances, 2015-01, Vol.5 (13), p.84432-84438</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-d248b379015f4f393842cb0f528f493360e180cfc25d1bfcea3fe7d5a678e73b3</citedby><cites>FETCH-LOGICAL-c345t-d248b379015f4f393842cb0f528f493360e180cfc25d1bfcea3fe7d5a678e73b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Cai, Minhua</creatorcontrib><creatorcontrib>He, Yuegui</creatorcontrib><creatorcontrib>Wang, Shuai</creatorcontrib><creatorcontrib>Zheng, Jinwang</creatorcontrib><creatorcontrib>Xu, Xiaoping</creatorcontrib><title>Development of antibacterial polyacrylonitrile membrane modified with a covalently immobilized lysozyme</title><title>RSC advances</title><description>A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared. First, the virginal PAN membranes were prepared
via
the classic immersion precipitation method. After modification with NaOH, HCl, ethylenediamine (EDA), the lysozyme was covalently immobilized onto the surface of the PAN membranes by glutaraldehyde. The chemical compositions of virginal and modified membranes were characterized by Fourier transform infrared spectroscopy (FT-IR) and Energy Dispersion X-ray (EDX). The morphology and performance of the immobilized membranes were characterized by Scanning Electronic Microscopy (SEM), filtration performance measurements, the amount of bonded lysozyme, lysozyme activity measurement and flow cytometry method. The antibacterial tests confirmed that the immobilized lysozyme membrane displayed an excellent antibacterial performance against
Staphylococcus aureus
(
S. aureus
).
A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared.</description><subject>Antiinfectives and antibacterials</subject><subject>Covalence</subject><subject>Dispersions</subject><subject>Electronics</subject><subject>Flow cytometry</subject><subject>Lysozyme</subject><subject>Membranes</subject><subject>Polyacrylonitriles</subject><subject>Staphylococcus</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkc1LAzEQxYMoWGov3oV4E2E1H5tk91iqVqEgiJ6XbDapkWyzJtvK9q83WlFP4lxm4P14M7wB4BijC4xoealYkDgvuFjugRFBOc8I4uX-r_kQTGJ8Qak4w4TjEVhe6Y12vmv1qofeQLnqbS1Vr4OVDnbeDVKFwfmV7YN1Gra6rYNcpcE31ljdwDfbP0MJld9Il0zcAG3b-to6u02qG6LfDq0-AgdGuqgnX30Mnm6uH2e32eJ-fjebLjJFc9ZnDcmLmooSYWZyQ0ta5ETVyDBSmLyklCONC6SMIqzBtVFaUqNFwyQXhRa0pmNwtvPtgn9d69hXrY1KO5du9utYYcEJKhMt_oESwQtB0toxON-hKvgYgzZVF2wrw1BhVH1kX83Yw_Qz-3mCT3ZwiOqb-_lN0k__0quuMfQdqzWOKw</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Liu, Wei</creator><creator>Cai, Minhua</creator><creator>He, Yuegui</creator><creator>Wang, Shuai</creator><creator>Zheng, Jinwang</creator><creator>Xu, Xiaoping</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20150101</creationdate><title>Development of antibacterial polyacrylonitrile membrane modified with a covalently immobilized lysozyme</title><author>Liu, Wei ; Cai, Minhua ; He, Yuegui ; Wang, Shuai ; Zheng, Jinwang ; Xu, Xiaoping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-d248b379015f4f393842cb0f528f493360e180cfc25d1bfcea3fe7d5a678e73b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Covalence</topic><topic>Dispersions</topic><topic>Electronics</topic><topic>Flow cytometry</topic><topic>Lysozyme</topic><topic>Membranes</topic><topic>Polyacrylonitriles</topic><topic>Staphylococcus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Cai, Minhua</creatorcontrib><creatorcontrib>He, Yuegui</creatorcontrib><creatorcontrib>Wang, Shuai</creatorcontrib><creatorcontrib>Zheng, Jinwang</creatorcontrib><creatorcontrib>Xu, Xiaoping</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Wei</au><au>Cai, Minhua</au><au>He, Yuegui</au><au>Wang, Shuai</au><au>Zheng, Jinwang</au><au>Xu, Xiaoping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of antibacterial polyacrylonitrile membrane modified with a covalently immobilized lysozyme</atitle><jtitle>RSC advances</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>13</issue><spage>84432</spage><epage>84438</epage><pages>84432-84438</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared. First, the virginal PAN membranes were prepared
via
the classic immersion precipitation method. After modification with NaOH, HCl, ethylenediamine (EDA), the lysozyme was covalently immobilized onto the surface of the PAN membranes by glutaraldehyde. The chemical compositions of virginal and modified membranes were characterized by Fourier transform infrared spectroscopy (FT-IR) and Energy Dispersion X-ray (EDX). The morphology and performance of the immobilized membranes were characterized by Scanning Electronic Microscopy (SEM), filtration performance measurements, the amount of bonded lysozyme, lysozyme activity measurement and flow cytometry method. The antibacterial tests confirmed that the immobilized lysozyme membrane displayed an excellent antibacterial performance against
Staphylococcus aureus
(
S. aureus
).
A novel antibacterial polyacrylonitrile (PAN) membrane covalently immobilized with lysozyme was prepared.</abstract><doi>10.1039/c5ra14867g</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2046-2069 |
| ispartof | RSC advances, 2015-01, Vol.5 (13), p.84432-84438 |
| issn | 2046-2069 2046-2069 |
| language | eng |
| recordid | cdi_rsc_primary_c5ra14867g |
| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Antiinfectives and antibacterials Covalence Dispersions Electronics Flow cytometry Lysozyme Membranes Polyacrylonitriles Staphylococcus |
| title | Development of antibacterial polyacrylonitrile membrane modified with a covalently immobilized lysozyme |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T18%3A18%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_rsc_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20antibacterial%20polyacrylonitrile%20membrane%20modified%20with%20a%20covalently%20immobilized%20lysozyme&rft.jtitle=RSC%20advances&rft.au=Liu,%20Wei&rft.date=2015-01-01&rft.volume=5&rft.issue=13&rft.spage=84432&rft.epage=84438&rft.pages=84432-84438&rft.issn=2046-2069&rft.eissn=2046-2069&rft_id=info:doi/10.1039/c5ra14867g&rft_dat=%3Cproquest_rsc_p%3E1727687293%3C/proquest_rsc_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1727687293&rft_id=info:pmid/&rfr_iscdi=true |