A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00681c

A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00681c

A rapid and accurate method to identify severe sepsis patients at high risk of death is critically needed for clinical practice. In a recent study, the concentration of cell-free DNA (cfDNA) in blood was found to be a prognostic indicator for ICU mortality in patients with severe sepsis. However, cu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Yang, Jun, Selvaganapathy, P. Ravi, Gould, Travis J, Dwivedi, Dhruva J, Liu, Dingsheng, Fox-Robichaud, Alison E, Liaw, Patricia C
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 3933
container_issue 19
container_start_page 3925
container_title
container_volume 15
creator Yang, Jun
Selvaganapathy, P. Ravi
Gould, Travis J
Dwivedi, Dhruva J
Liu, Dingsheng
Fox-Robichaud, Alison E
Liaw, Patricia C
description A rapid and accurate method to identify severe sepsis patients at high risk of death is critically needed for clinical practice. In a recent study, the concentration of cell-free DNA (cfDNA) in blood was found to be a prognostic indicator for ICU mortality in patients with severe sepsis. However, current DNA quantification techniques are time-consuming and involve extensive sample preparation. In this paper, we demonstrate a low-cost microfluidic device capable of rapid quantification of cfDNA in a small droplet (
doi_str_mv 10.1039/c5lc00681c
format Article
fullrecord <record><control><sourceid>rsc</sourceid><recordid>TN_cdi_rsc_primary_c5lc00681c</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>c5lc00681c</sourcerecordid><originalsourceid>FETCH-rsc_primary_c5lc00681c3</originalsourceid><addsrcrecordid>eNqFjz1LBDEQhoMoeH409sLYabFnwt7Hrt2hK16jxdkvMTvBkWwSk-yKv8q_aETRQtDqHXjeeYZh7EjwqeBlfa7mRnG-qITaYhMxW5YFF1W9_T3Xy122F-MT52I-W1QT9raCnlRw2gzUkYIOR1II2gUI0lMHz4O0iTQpmchZcBoUGlPogAhXtysgCz4jtCnCC6VHiDhiwBw-UmwMqhSczeY4eG-wz0UZXvNaPtF_Ok-bzfoM5CjJyAeDU9h8uO_WF_D7rQO2o6WJePiV--z4urm_vClCVK0P1Gd5-1Mv_-cnf_HWd7p8BzQlbCQ</addsrcrecordid><sourcetype>Enrichment Source</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00681c</title><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Yang, Jun ; Selvaganapathy, P. Ravi ; Gould, Travis J ; Dwivedi, Dhruva J ; Liu, Dingsheng ; Fox-Robichaud, Alison E ; Liaw, Patricia C</creator><creatorcontrib>Yang, Jun ; Selvaganapathy, P. Ravi ; Gould, Travis J ; Dwivedi, Dhruva J ; Liu, Dingsheng ; Fox-Robichaud, Alison E ; Liaw, Patricia C</creatorcontrib><description>A rapid and accurate method to identify severe sepsis patients at high risk of death is critically needed for clinical practice. In a recent study, the concentration of cell-free DNA (cfDNA) in blood was found to be a prognostic indicator for ICU mortality in patients with severe sepsis. However, current DNA quantification techniques are time-consuming and involve extensive sample preparation. In this paper, we demonstrate a low-cost microfluidic device capable of rapid quantification of cfDNA in a small droplet (&lt;10 μl) of blood plasma and whole blood in 5 min using only electrical power. The cfDNA in samples is selectively labeled by PicoGreen and is extracted and concentrated by electrophoresis into a gel by application of a DC potential of 9 V. This device has potential as a prognostic tool for early and rapid assessment of septic patients. Microfluidic chip for rapid, reagent-free quantification of cell-free DNA in blood for severe sepsis prognostics.</description><identifier>ISSN: 1473-0197</identifier><identifier>EISSN: 1473-0189</identifier><identifier>DOI: 10.1039/c5lc00681c</identifier><language>eng</language><creationdate>2015-09</creationdate><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yang, Jun</creatorcontrib><creatorcontrib>Selvaganapathy, P. Ravi</creatorcontrib><creatorcontrib>Gould, Travis J</creatorcontrib><creatorcontrib>Dwivedi, Dhruva J</creatorcontrib><creatorcontrib>Liu, Dingsheng</creatorcontrib><creatorcontrib>Fox-Robichaud, Alison E</creatorcontrib><creatorcontrib>Liaw, Patricia C</creatorcontrib><title>A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00681c</title><description>A rapid and accurate method to identify severe sepsis patients at high risk of death is critically needed for clinical practice. In a recent study, the concentration of cell-free DNA (cfDNA) in blood was found to be a prognostic indicator for ICU mortality in patients with severe sepsis. However, current DNA quantification techniques are time-consuming and involve extensive sample preparation. In this paper, we demonstrate a low-cost microfluidic device capable of rapid quantification of cfDNA in a small droplet (&lt;10 μl) of blood plasma and whole blood in 5 min using only electrical power. The cfDNA in samples is selectively labeled by PicoGreen and is extracted and concentrated by electrophoresis into a gel by application of a DC potential of 9 V. This device has potential as a prognostic tool for early and rapid assessment of septic patients. Microfluidic chip for rapid, reagent-free quantification of cell-free DNA in blood for severe sepsis prognostics.</description><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqFjz1LBDEQhoMoeH409sLYabFnwt7Hrt2hK16jxdkvMTvBkWwSk-yKv8q_aETRQtDqHXjeeYZh7EjwqeBlfa7mRnG-qITaYhMxW5YFF1W9_T3Xy122F-MT52I-W1QT9raCnlRw2gzUkYIOR1II2gUI0lMHz4O0iTQpmchZcBoUGlPogAhXtysgCz4jtCnCC6VHiDhiwBw-UmwMqhSczeY4eG-wz0UZXvNaPtF_Ok-bzfoM5CjJyAeDU9h8uO_WF_D7rQO2o6WJePiV--z4urm_vClCVK0P1Gd5-1Mv_-cnf_HWd7p8BzQlbCQ</recordid><startdate>20150916</startdate><enddate>20150916</enddate><creator>Yang, Jun</creator><creator>Selvaganapathy, P. Ravi</creator><creator>Gould, Travis J</creator><creator>Dwivedi, Dhruva J</creator><creator>Liu, Dingsheng</creator><creator>Fox-Robichaud, Alison E</creator><creator>Liaw, Patricia C</creator><scope/></search><sort><creationdate>20150916</creationdate><title>A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00681c</title><author>Yang, Jun ; Selvaganapathy, P. Ravi ; Gould, Travis J ; Dwivedi, Dhruva J ; Liu, Dingsheng ; Fox-Robichaud, Alison E ; Liaw, Patricia C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-rsc_primary_c5lc00681c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Jun</creatorcontrib><creatorcontrib>Selvaganapathy, P. Ravi</creatorcontrib><creatorcontrib>Gould, Travis J</creatorcontrib><creatorcontrib>Dwivedi, Dhruva J</creatorcontrib><creatorcontrib>Liu, Dingsheng</creatorcontrib><creatorcontrib>Fox-Robichaud, Alison E</creatorcontrib><creatorcontrib>Liaw, Patricia C</creatorcontrib></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Jun</au><au>Selvaganapathy, P. Ravi</au><au>Gould, Travis J</au><au>Dwivedi, Dhruva J</au><au>Liu, Dingsheng</au><au>Fox-Robichaud, Alison E</au><au>Liaw, Patricia C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00681c</atitle><date>2015-09-16</date><risdate>2015</risdate><volume>15</volume><issue>19</issue><spage>3925</spage><epage>3933</epage><pages>3925-3933</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>A rapid and accurate method to identify severe sepsis patients at high risk of death is critically needed for clinical practice. In a recent study, the concentration of cell-free DNA (cfDNA) in blood was found to be a prognostic indicator for ICU mortality in patients with severe sepsis. However, current DNA quantification techniques are time-consuming and involve extensive sample preparation. In this paper, we demonstrate a low-cost microfluidic device capable of rapid quantification of cfDNA in a small droplet (&lt;10 μl) of blood plasma and whole blood in 5 min using only electrical power. The cfDNA in samples is selectively labeled by PicoGreen and is extracted and concentrated by electrophoresis into a gel by application of a DC potential of 9 V. This device has potential as a prognostic tool for early and rapid assessment of septic patients. Microfluidic chip for rapid, reagent-free quantification of cell-free DNA in blood for severe sepsis prognostics.</abstract><doi>10.1039/c5lc00681c</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1473-0197
ispartof
issn 1473-0197
1473-0189
language eng
recordid cdi_rsc_primary_c5lc00681c
source Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
title A microfluidic device for rapid quantification of cell-free DNA in patients with severe sepsisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00681c
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T20%3A36%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-rsc&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20microfluidic%20device%20for%20rapid%20quantification%20of%20cell-free%20DNA%20in%20patients%20with%20severe%20sepsisElectronic%20supplementary%20information%20(ESI)%20available.%20See%20DOI:%2010.1039/c5lc00681c&rft.au=Yang,%20Jun&rft.date=2015-09-16&rft.volume=15&rft.issue=19&rft.spage=3925&rft.epage=3933&rft.pages=3925-3933&rft.issn=1473-0197&rft.eissn=1473-0189&rft_id=info:doi/10.1039/c5lc00681c&rft_dat=%3Crsc%3Ec5lc00681c%3C/rsc%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true