Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activityElectronic supplementary information (ESI) available: Synthetic experimental details and additional figures and tables. See DOI: 10.1039/c5cc02450a

Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activityElectronic supplementary information (ESI) available: Synthetic experimental details and additional figures and tables. See DOI: 10.1039/c5cc02450a

A solid-phase strategy using lipase as a biomolecular scaffold to produce a large amount of Cu 2+ -metalloenzyme is proposed here. The application of this protocol on different 3D cavities of the enzyme allows creating a heterogeneous artificial metallolipase showing chimeric catalytic activity. The...

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Hauptverfasser: Filice, M, Romero, O, Gutiérrez-Fernández, J, de las Rivas, B, Hermoso, J. A, Palomo, J. M
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creator Filice, M
Romero, O
Gutiérrez-Fernández, J
de las Rivas, B
Hermoso, J. A
Palomo, J. M
description A solid-phase strategy using lipase as a biomolecular scaffold to produce a large amount of Cu 2+ -metalloenzyme is proposed here. The application of this protocol on different 3D cavities of the enzyme allows creating a heterogeneous artificial metallolipase showing chimeric catalytic activity. The artificial catalyst was assessed in Diels-Alder cycloaddition reactions and cascade reactions showing excellent catalytic properties. The practical synthesis in high overall yields of a heterogeneous artificial copper-lipase with chimeric catalytic activity (native plus artificial) is presented here. This novel hybrid catalyst showed excellent catalytic properties in Diels-Alder and cascade reactions.
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title Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activityElectronic supplementary information (ESI) available: Synthetic experimental details and additional figures and tables. See DOI: 10.1039/c5cc02450a
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