Lipid-conjugated fluorescent pH sensors for monitoring pH changes in reconstituted membrane systemsElectronic supplementary information (ESI) available: Scheme of SNARF conjugation to DOPE is shown (Fig. S1), head group lipid composition of lecithin is demonstrated (Fig. S2), fluorescence emission spectra of soluble SNARF in buffer solutions of different pH are presented (Fig. S3), size distribution of liposomes containing pH-sensors (Fig. S4), comparison of relative fluorescence change Pyranine

Lipid-conjugated fluorescent pH sensors for monitoring pH changes in reconstituted membrane systemsElectronic supplementary information (ESI) available: Scheme of SNARF conjugation to DOPE is shown (Fig. S1), head group lipid composition of lecithin is demonstrated (Fig. S2), fluorescence emission spectra of soluble SNARF in buffer solutions of different pH are presented (Fig. S3), size distribution of liposomes containing pH-sensors (Fig. S4), comparison of relative fluorescence change Pyranine

Accurate real-time measurements of the dynamics of proton concentration gradients are crucial for detailed molecular studies of proton translocation by membrane-bound enzymes. To reduce complexity, these measurements are often carried out with purified, reconstituted enzyme systems. Yet the most par...

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Hauptverfasser: Kemmer, Gerdi Christine, Bogh, Sidsel Ammitzbøll, Urban, Michael, Palmgren, Michael G, Vosch, Tom, Schiller, Jürgen, Günther Pomorski, Thomas
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Sprache:eng
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