Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probeElectronic supplementary information (ESI) available: Compound characterisation and supplementary data. See DOI: 10.1039/c4sc03319a
A series of luminescent Ir( iii ) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir( iii ) complex 9 , [Ir(phq) 2 (phen)]PF 6 (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence...
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| creator | Leung, Ka-Ho He, Hong-Zhang He, Bingyong Zhong, Hai-Jing Lin, Sheng Wang, Yi-Tao Ma, Dik-Lung Leung, Chung-Hang |
| description | A series of luminescent Ir(
iii
) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(
iii
) complex
9
, [Ir(phq)
2
(phen)]PF
6
(where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.
A novel luminescent G-quadruplex-selective iridium(
iii
) complex was employed in a label-free G-quadruplex-based detection assay for hepatitis C virus NS3 helicase activity. |
| doi_str_mv | 10.1039/c4sc03319a |
| format | Article |
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iii
) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(
iii
) complex
9
, [Ir(phq)
2
(phen)]PF
6
(where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.
A novel luminescent G-quadruplex-selective iridium(
iii
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iii
) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(
iii
) complex
9
, [Ir(phq)
2
(phen)]PF
6
(where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.
A novel luminescent G-quadruplex-selective iridium(
iii
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iii
) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(
iii
) complex
9
, [Ir(phq)
2
(phen)]PF
6
(where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.
A novel luminescent G-quadruplex-selective iridium(
iii
) complex was employed in a label-free G-quadruplex-based detection assay for hepatitis C virus NS3 helicase activity.</abstract><doi>10.1039/c4sc03319a</doi><tpages>6</tpages></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| title | Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probeElectronic supplementary information (ESI) available: Compound characterisation and supplementary data. See DOI: 10.1039/c4sc03319a |
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