Singlet oxygen photosensitisation by the fluorescent protein Pp2FbFP L30M, a novel derivative of Pseudomonas putida flavin-binding Pp2FbFPDedicated to Professor Kristian Berg on the occasion of his 60th birthday.Electronic supplementary information (ESI) available: Pp2FbFP L30M 1O2 phosphorescence kinetics; effect of oxygen concentration on the rate of Pp2FbFP L30M triplet state decay; transient absorbance decays; deconvolution of 1O2 phosphorescence. See DOI: 10.1039/c4pp00338a
Flavin-binding fluorescent proteins (FbFPs) are a class of fluorescent reporters that have been increasingly used as reporters in the study of cellular structures and dynamics. Flavin's intrinsic high singlet oxygen ( 1 O 2 ) quantum yield ( Φ Δ = 0.51) provides a basis for the development of n...
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