Grafting synthetic transmembrane units to the engineered low-toxicity α-hemolysin to restore its hemolytic activityElectronic supplementary information (ESI) available: Size-exclusion chromatography and activity assay of Hla mutants, SDS-PAGE and western blot analysis, TEM images, EPR spectra, conductance measurements, absorption, fluorescence, and CD spectra. See DOI: 10.1039/c4mb00405a

The chemical modification of proteins to provide desirable functions and/or structures broadens their possibilities for use in various applications. Usually, proteins can acquire new functions and characteristics, in addition to their original ones, via the introduction of synthetic functional moiet...

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Hauptverfasser: Ui, Mihoko, Harima, Kousuke, Takei, Toshiaki, Tsumoto, Kouhei, Tabata, Kazuhito V, Noji, Hiroyuki, Endo, Sumire, Akiyama, Kimio, Muraoka, Takahiro, Kinbara, Kazushi
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Sprache:eng
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