Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenaseElectronic supplementary information (ESI) available. See DOI: 10.1039/c4cc08802f
Mutants of toluene o -xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying o...
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| creator | Carlin, D. A Bertolani, S. J Siegel, J. B |
| description | Mutants of toluene
o
-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide
in vivo
at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.
We report the first recombinant enzyme in
E. coli
capable of transforming ethylene to ethylene oxide. |
| doi_str_mv | 10.1039/c4cc08802f |
| format | Article |
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o
-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide
in vivo
at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.
We report the first recombinant enzyme in
E. coli
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o
-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide
in vivo
at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.
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E. coli
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o
-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide
in vivo
at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.
We report the first recombinant enzyme in
E. coli
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| source | Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| title | Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenaseElectronic supplementary information (ESI) available. See DOI: 10.1039/c4cc08802f |
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