Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)Electronic supplementary information (ESI) available: Experimental section. See DOI: 10.1039/c4cc00540f

Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)Electronic supplementary information (ESI) available: Experimental section. See DOI: 10.1039/c4cc00540f

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples. Supplementing loop-mediated isothermal...

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Hauptverfasser: Hsieh, Kuangwen, Mage, Peter L, Csordas, Andrew T, Eisenstein, Michael, Tom Soh, H
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creator Hsieh, Kuangwen
Mage, Peter L
Csordas, Andrew T
Eisenstein, Michael
Tom Soh, H
description We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples. Supplementing loop-mediated isothermal amplification (LAMP) reactions with uracil-DNA-glycosylase enables simultaneous elimination of carryover contamination and detection of target DNA.
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title Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)Electronic supplementary information (ESI) available: Experimental section. See DOI: 10.1039/c4cc00540f
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