Back to the roots: photodynamic inactivation of bacteria based on water-soluble curcumin bound to polyvinylpyrrolidone as a photosensitizer
Photodynamic inactivation (PDI), the light-induced and photosensitizer-mediated overproduction of reactive oxygen species in microorganisms, represents a convincing approach to treat infections with (multi-resistant) pathogens. Due to its favourable photoactive properties combined with excellent bio...
Gespeichert in:
| Veröffentlicht in: | Photochemical & photobiological sciences 2013-10, Vol.12 (1), p.1795-182 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 182 |
|---|---|
| container_issue | 1 |
| container_start_page | 1795 |
| container_title | Photochemical & photobiological sciences |
| container_volume | 12 |
| creator | Winter, Sandra Tortik, Nicole Kubin, Andreas Krammer, Barbara Plaetzer, Kristjan |
| description | Photodynamic inactivation (PDI), the light-induced and photosensitizer-mediated overproduction of reactive oxygen species in microorganisms, represents a convincing approach to treat infections with (multi-resistant) pathogens. Due to its favourable photoactive properties combined with excellent biocompatibility, curcumin derived from the roots of turmeric (
Curcuma longa
) has been identified as an advantageous photosensitizer for PDI. To overcome the poor water solubility and the rapid decay of the natural substance at physiological pH, we examined the applicability of polyvinylpyrrolidone curcumin (PVP-C) in an acidified aqueous solution (solubility of PVP-C up to 2.7 mM) for photoinactivation of Gram(+) and Gram(−) bacteria. Five micromolar PVP-C incubated for 5 minutes and illuminated using a blue light LED array (435 ± 10 nm, 33.8 J cm
−2
) resulted in a >6 log
10
reduction of the number of viable
Staphylococcus aureus
. At this concentration, longer incubation periods result in a lower phototoxicity, most likely due to degeneration of curcumin. Upon an increase of the PVP-C concentration to 50 μM (incubation for 15 or 25 min) a complete eradication of
Staphylococcus aureus
can be achieved. As expected for a non-cationic photosensitizer, cell wall permeabilization with CaCl
2
prior to addition of 50 μM PVP-C for 15 min is necessary to induce a drop in the count of the Gram(−)
Escherichia coli
for more than 3 log
10
. As both constituents of the formulation, curcumin (E number E100) and polyvinylpyrrolidone (E1201), have been approved as food additives, a PDI based on PCP-C might allow for a very sparing clinical application (
e.g.
for disinfection of wounds) or even for employment in aseptic production of foodstuffs.
Photodynamic inactivation based on curcumin bound to polyvinylpyrrolidone is highly effective against
Staphylococcus aureus
and
Escherichia coli
. The novel formulation provides high water-solubility combined with the excellent bio-compatibility of curcumin. |
| doi_str_mv | 10.1039/c3pp50095k |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_rsc_p</sourceid><recordid>TN_cdi_rsc_primary_c3pp50095k</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1436566727</sourcerecordid><originalsourceid>FETCH-LOGICAL-c470t-9b3ff3c35efca2ec4ea8ed225d4521ed33a9efae8088e16163361603fb0ce8273</originalsourceid><addsrcrecordid>eNqNkV1r1zAUh4M43JzeeK_EO3FU89K0qXc6dAoDbxS8K2ly4rK1SZekk_oV_NLL7PyLIOJNcnJ-D8-BHIQeUfKCEt691HyeBSGduLiDDmjd1lVHOnZ3V4sv--h-SueEUFE37T20z7hkkpP2AP14o_QFzgHnM8AxhJxe4fks5GBWryansfNKZ3elsgseB4uH8oToVCkSGFya31RpVCmMyzAC1kvUy-Q8HsLizY15DuN65fw6zmuMYXQmeMAqYbUNSuCTy-47xAdoz6oxwcPb-xB9fvf20_H76vTjyYfj16eVrluSq27g1nLNBVitGOgalATDmDC1YBQM56oDq0ASKYE2tOG8HITbgWiQrOWH6NnmnWO4XCDlfnJJwzgqD2FJPa1ryRiRgv4HyhvRNO1P6_MN1TGkFMH2c3STimtPSX-zp_73ngr85Na7DBOYHfprMQU42oBUIv8VYn8elujLt_xd93ijY9I71x_503_l_WwsvwbJ2bXX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1436566727</pqid></control><display><type>article</type><title>Back to the roots: photodynamic inactivation of bacteria based on water-soluble curcumin bound to polyvinylpyrrolidone as a photosensitizer</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals</source><source>SpringerLink Journals - AutoHoldings</source><creator>Winter, Sandra ; Tortik, Nicole ; Kubin, Andreas ; Krammer, Barbara ; Plaetzer, Kristjan</creator><creatorcontrib>Winter, Sandra ; Tortik, Nicole ; Kubin, Andreas ; Krammer, Barbara ; Plaetzer, Kristjan</creatorcontrib><description>Photodynamic inactivation (PDI), the light-induced and photosensitizer-mediated overproduction of reactive oxygen species in microorganisms, represents a convincing approach to treat infections with (multi-resistant) pathogens. Due to its favourable photoactive properties combined with excellent biocompatibility, curcumin derived from the roots of turmeric (
Curcuma longa
) has been identified as an advantageous photosensitizer for PDI. To overcome the poor water solubility and the rapid decay of the natural substance at physiological pH, we examined the applicability of polyvinylpyrrolidone curcumin (PVP-C) in an acidified aqueous solution (solubility of PVP-C up to 2.7 mM) for photoinactivation of Gram(+) and Gram(−) bacteria. Five micromolar PVP-C incubated for 5 minutes and illuminated using a blue light LED array (435 ± 10 nm, 33.8 J cm
−2
) resulted in a >6 log
10
reduction of the number of viable
Staphylococcus aureus
. At this concentration, longer incubation periods result in a lower phototoxicity, most likely due to degeneration of curcumin. Upon an increase of the PVP-C concentration to 50 μM (incubation for 15 or 25 min) a complete eradication of
Staphylococcus aureus
can be achieved. As expected for a non-cationic photosensitizer, cell wall permeabilization with CaCl
2
prior to addition of 50 μM PVP-C for 15 min is necessary to induce a drop in the count of the Gram(−)
Escherichia coli
for more than 3 log
10
. As both constituents of the formulation, curcumin (E number E100) and polyvinylpyrrolidone (E1201), have been approved as food additives, a PDI based on PCP-C might allow for a very sparing clinical application (
e.g.
for disinfection of wounds) or even for employment in aseptic production of foodstuffs.
Photodynamic inactivation based on curcumin bound to polyvinylpyrrolidone is highly effective against
Staphylococcus aureus
and
Escherichia coli
. The novel formulation provides high water-solubility combined with the excellent bio-compatibility of curcumin.</description><identifier>ISSN: 1474-905X</identifier><identifier>EISSN: 1474-9092</identifier><identifier>DOI: 10.1039/c3pp50095k</identifier><identifier>PMID: 23828307</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Biochemistry ; Biocompatibility ; Biomaterials ; Calcium Chloride - chemistry ; Calcium Chloride - pharmacology ; Cell Wall - drug effects ; Chemistry ; Curcuma - chemistry ; Curcuma - metabolism ; Curcuma longa ; Curcumin - chemistry ; Escherichia coli - drug effects ; Escherichia coli - radiation effects ; Hydrogen-Ion Concentration ; Light ; Photosensitizing Agents - chemistry ; Photosensitizing Agents - pharmacology ; Physical Chemistry ; Plant Roots - chemistry ; Plant Roots - metabolism ; Plant Sciences ; Povidone - chemistry ; Solubility ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - radiation effects ; Water - chemistry</subject><ispartof>Photochemical & photobiological sciences, 2013-10, Vol.12 (1), p.1795-182</ispartof><rights>The Royal Society of Chemistry and Owner Societies 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-9b3ff3c35efca2ec4ea8ed225d4521ed33a9efae8088e16163361603fb0ce8273</citedby><cites>FETCH-LOGICAL-c470t-9b3ff3c35efca2ec4ea8ed225d4521ed33a9efae8088e16163361603fb0ce8273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1039/c3pp50095k$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1039/c3pp50095k$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23828307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Winter, Sandra</creatorcontrib><creatorcontrib>Tortik, Nicole</creatorcontrib><creatorcontrib>Kubin, Andreas</creatorcontrib><creatorcontrib>Krammer, Barbara</creatorcontrib><creatorcontrib>Plaetzer, Kristjan</creatorcontrib><title>Back to the roots: photodynamic inactivation of bacteria based on water-soluble curcumin bound to polyvinylpyrrolidone as a photosensitizer</title><title>Photochemical & photobiological sciences</title><addtitle>Photochem Photobiol Sci</addtitle><addtitle>Photochem Photobiol Sci</addtitle><description>Photodynamic inactivation (PDI), the light-induced and photosensitizer-mediated overproduction of reactive oxygen species in microorganisms, represents a convincing approach to treat infections with (multi-resistant) pathogens. Due to its favourable photoactive properties combined with excellent biocompatibility, curcumin derived from the roots of turmeric (
Curcuma longa
) has been identified as an advantageous photosensitizer for PDI. To overcome the poor water solubility and the rapid decay of the natural substance at physiological pH, we examined the applicability of polyvinylpyrrolidone curcumin (PVP-C) in an acidified aqueous solution (solubility of PVP-C up to 2.7 mM) for photoinactivation of Gram(+) and Gram(−) bacteria. Five micromolar PVP-C incubated for 5 minutes and illuminated using a blue light LED array (435 ± 10 nm, 33.8 J cm
−2
) resulted in a >6 log
10
reduction of the number of viable
Staphylococcus aureus
. At this concentration, longer incubation periods result in a lower phototoxicity, most likely due to degeneration of curcumin. Upon an increase of the PVP-C concentration to 50 μM (incubation for 15 or 25 min) a complete eradication of
Staphylococcus aureus
can be achieved. As expected for a non-cationic photosensitizer, cell wall permeabilization with CaCl
2
prior to addition of 50 μM PVP-C for 15 min is necessary to induce a drop in the count of the Gram(−)
Escherichia coli
for more than 3 log
10
. As both constituents of the formulation, curcumin (E number E100) and polyvinylpyrrolidone (E1201), have been approved as food additives, a PDI based on PCP-C might allow for a very sparing clinical application (
e.g.
for disinfection of wounds) or even for employment in aseptic production of foodstuffs.
Photodynamic inactivation based on curcumin bound to polyvinylpyrrolidone is highly effective against
Staphylococcus aureus
and
Escherichia coli
. The novel formulation provides high water-solubility combined with the excellent bio-compatibility of curcumin.</description><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biomaterials</subject><subject>Calcium Chloride - chemistry</subject><subject>Calcium Chloride - pharmacology</subject><subject>Cell Wall - drug effects</subject><subject>Chemistry</subject><subject>Curcuma - chemistry</subject><subject>Curcuma - metabolism</subject><subject>Curcuma longa</subject><subject>Curcumin - chemistry</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - radiation effects</subject><subject>Hydrogen-Ion Concentration</subject><subject>Light</subject><subject>Photosensitizing Agents - chemistry</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Physical Chemistry</subject><subject>Plant Roots - chemistry</subject><subject>Plant Roots - metabolism</subject><subject>Plant Sciences</subject><subject>Povidone - chemistry</subject><subject>Solubility</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - radiation effects</subject><subject>Water - chemistry</subject><issn>1474-905X</issn><issn>1474-9092</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1r1zAUh4M43JzeeK_EO3FU89K0qXc6dAoDbxS8K2ly4rK1SZekk_oV_NLL7PyLIOJNcnJ-D8-BHIQeUfKCEt691HyeBSGduLiDDmjd1lVHOnZ3V4sv--h-SueEUFE37T20z7hkkpP2AP14o_QFzgHnM8AxhJxe4fks5GBWryansfNKZ3elsgseB4uH8oToVCkSGFya31RpVCmMyzAC1kvUy-Q8HsLizY15DuN65fw6zmuMYXQmeMAqYbUNSuCTy-47xAdoz6oxwcPb-xB9fvf20_H76vTjyYfj16eVrluSq27g1nLNBVitGOgalATDmDC1YBQM56oDq0ASKYE2tOG8HITbgWiQrOWH6NnmnWO4XCDlfnJJwzgqD2FJPa1ryRiRgv4HyhvRNO1P6_MN1TGkFMH2c3STimtPSX-zp_73ngr85Na7DBOYHfprMQU42oBUIv8VYn8elujLt_xd93ijY9I71x_503_l_WwsvwbJ2bXX</recordid><startdate>201310</startdate><enddate>201310</enddate><creator>Winter, Sandra</creator><creator>Tortik, Nicole</creator><creator>Kubin, Andreas</creator><creator>Krammer, Barbara</creator><creator>Plaetzer, Kristjan</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201310</creationdate><title>Back to the roots: photodynamic inactivation of bacteria based on water-soluble curcumin bound to polyvinylpyrrolidone as a photosensitizer</title><author>Winter, Sandra ; Tortik, Nicole ; Kubin, Andreas ; Krammer, Barbara ; Plaetzer, Kristjan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-9b3ff3c35efca2ec4ea8ed225d4521ed33a9efae8088e16163361603fb0ce8273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biochemistry</topic><topic>Biocompatibility</topic><topic>Biomaterials</topic><topic>Calcium Chloride - chemistry</topic><topic>Calcium Chloride - pharmacology</topic><topic>Cell Wall - drug effects</topic><topic>Chemistry</topic><topic>Curcuma - chemistry</topic><topic>Curcuma - metabolism</topic><topic>Curcuma longa</topic><topic>Curcumin - chemistry</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - radiation effects</topic><topic>Hydrogen-Ion Concentration</topic><topic>Light</topic><topic>Photosensitizing Agents - chemistry</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Physical Chemistry</topic><topic>Plant Roots - chemistry</topic><topic>Plant Roots - metabolism</topic><topic>Plant Sciences</topic><topic>Povidone - chemistry</topic><topic>Solubility</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - radiation effects</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Winter, Sandra</creatorcontrib><creatorcontrib>Tortik, Nicole</creatorcontrib><creatorcontrib>Kubin, Andreas</creatorcontrib><creatorcontrib>Krammer, Barbara</creatorcontrib><creatorcontrib>Plaetzer, Kristjan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Photochemical & photobiological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Winter, Sandra</au><au>Tortik, Nicole</au><au>Kubin, Andreas</au><au>Krammer, Barbara</au><au>Plaetzer, Kristjan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Back to the roots: photodynamic inactivation of bacteria based on water-soluble curcumin bound to polyvinylpyrrolidone as a photosensitizer</atitle><jtitle>Photochemical & photobiological sciences</jtitle><stitle>Photochem Photobiol Sci</stitle><addtitle>Photochem Photobiol Sci</addtitle><date>2013-10</date><risdate>2013</risdate><volume>12</volume><issue>1</issue><spage>1795</spage><epage>182</epage><pages>1795-182</pages><issn>1474-905X</issn><eissn>1474-9092</eissn><abstract>Photodynamic inactivation (PDI), the light-induced and photosensitizer-mediated overproduction of reactive oxygen species in microorganisms, represents a convincing approach to treat infections with (multi-resistant) pathogens. Due to its favourable photoactive properties combined with excellent biocompatibility, curcumin derived from the roots of turmeric (
Curcuma longa
) has been identified as an advantageous photosensitizer for PDI. To overcome the poor water solubility and the rapid decay of the natural substance at physiological pH, we examined the applicability of polyvinylpyrrolidone curcumin (PVP-C) in an acidified aqueous solution (solubility of PVP-C up to 2.7 mM) for photoinactivation of Gram(+) and Gram(−) bacteria. Five micromolar PVP-C incubated for 5 minutes and illuminated using a blue light LED array (435 ± 10 nm, 33.8 J cm
−2
) resulted in a >6 log
10
reduction of the number of viable
Staphylococcus aureus
. At this concentration, longer incubation periods result in a lower phototoxicity, most likely due to degeneration of curcumin. Upon an increase of the PVP-C concentration to 50 μM (incubation for 15 or 25 min) a complete eradication of
Staphylococcus aureus
can be achieved. As expected for a non-cationic photosensitizer, cell wall permeabilization with CaCl
2
prior to addition of 50 μM PVP-C for 15 min is necessary to induce a drop in the count of the Gram(−)
Escherichia coli
for more than 3 log
10
. As both constituents of the formulation, curcumin (E number E100) and polyvinylpyrrolidone (E1201), have been approved as food additives, a PDI based on PCP-C might allow for a very sparing clinical application (
e.g.
for disinfection of wounds) or even for employment in aseptic production of foodstuffs.
Photodynamic inactivation based on curcumin bound to polyvinylpyrrolidone is highly effective against
Staphylococcus aureus
and
Escherichia coli
. The novel formulation provides high water-solubility combined with the excellent bio-compatibility of curcumin.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>23828307</pmid><doi>10.1039/c3pp50095k</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1474-905X |
| ispartof | Photochemical & photobiological sciences, 2013-10, Vol.12 (1), p.1795-182 |
| issn | 1474-905X 1474-9092 |
| language | eng |
| recordid | cdi_rsc_primary_c3pp50095k |
| source | MEDLINE; Royal Society Of Chemistry Journals; SpringerLink Journals - AutoHoldings |
| subjects | Biochemistry Biocompatibility Biomaterials Calcium Chloride - chemistry Calcium Chloride - pharmacology Cell Wall - drug effects Chemistry Curcuma - chemistry Curcuma - metabolism Curcuma longa Curcumin - chemistry Escherichia coli - drug effects Escherichia coli - radiation effects Hydrogen-Ion Concentration Light Photosensitizing Agents - chemistry Photosensitizing Agents - pharmacology Physical Chemistry Plant Roots - chemistry Plant Roots - metabolism Plant Sciences Povidone - chemistry Solubility Staphylococcus aureus - drug effects Staphylococcus aureus - radiation effects Water - chemistry |
| title | Back to the roots: photodynamic inactivation of bacteria based on water-soluble curcumin bound to polyvinylpyrrolidone as a photosensitizer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T06%3A23%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_rsc_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Back%20to%20the%20roots:%20photodynamic%20inactivation%20of%20bacteria%20based%20on%20water-soluble%20curcumin%20bound%20to%20polyvinylpyrrolidone%20as%20a%20photosensitizer&rft.jtitle=Photochemical%20&%20photobiological%20sciences&rft.au=Winter,%20Sandra&rft.date=2013-10&rft.volume=12&rft.issue=1&rft.spage=1795&rft.epage=182&rft.pages=1795-182&rft.issn=1474-905X&rft.eissn=1474-9092&rft_id=info:doi/10.1039/c3pp50095k&rft_dat=%3Cproquest_rsc_p%3E1436566727%3C/proquest_rsc_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1436566727&rft_id=info:pmid/23828307&rfr_iscdi=true |